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ARTICLE, Cellular/Molecular

The Binding of 2-(4′-Methylaminophenyl)Benzothiazole to Postmortem Brain Homogenates Is Dominated by the Amyloid Component

William E. Klunk, Yanming Wang, Guo-feng Huang, Manik L. Debnath, Daniel P. Holt, Li Shao, Ronald L. Hamilton, Milos D. Ikonomovic, Steven T. DeKosky and Chester A. Mathis
Journal of Neuroscience 15 March 2003, 23 (6) 2086-2092; https://doi.org/10.1523/JNEUROSCI.23-06-02086.2003
William E. Klunk
1Laboratory of Molecular Neuropharmacology, Department of Psychiatry, Western Psychiatric Institute and Clinic,
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Yanming Wang
2PET Facility, Department of Radiology,
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Guo-feng Huang
2PET Facility, Department of Radiology,
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Manik L. Debnath
1Laboratory of Molecular Neuropharmacology, Department of Psychiatry, Western Psychiatric Institute and Clinic,
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Daniel P. Holt
2PET Facility, Department of Radiology,
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Li Shao
1Laboratory of Molecular Neuropharmacology, Department of Psychiatry, Western Psychiatric Institute and Clinic,
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Ronald L. Hamilton
3Division of Neuropathology, Department of Pathology, and
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Milos D. Ikonomovic
4Department of Neurology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213
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Steven T. DeKosky
4Department of Neurology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213
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Chester A. Mathis
2PET Facility, Department of Radiology,
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.23-06-02086.2003
PubMed 
12657667
Published By 
Society for Neuroscience
History 
  • Received October 3, 2002
  • Revision received November 26, 2002
  • Accepted January 8, 2003
  • First published March 15, 2003.
  • Version of record published March 15, 2003.
Copyright & Usage 
Copyright © 2003 Society for Neuroscience

Author Information

  1. William E. Klunk1,
  2. Yanming Wang2,
  3. Guo-feng Huang2,
  4. Manik L. Debnath1,
  5. Daniel P. Holt2,
  6. Li Shao1,
  7. Ronald L. Hamilton3,
  8. Milos D. Ikonomovic4,
  9. Steven T. DeKosky4, and
  10. Chester A. Mathis2
  1. 1Laboratory of Molecular Neuropharmacology, Department of Psychiatry, Western Psychiatric Institute and Clinic,
  2. 2PET Facility, Department of Radiology,
  3. 3Division of Neuropathology, Department of Pathology, and
  4. 4Department of Neurology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213
View Full Text

Author contributions

Disclosures

    • Received October 3, 2002.
    • Revision received November 26, 2002.
    • Accepted January 8, 2003.
  • This work was supported in part by the Alzheimer's Association (Grants IIRG-95-076 and TLL-01-3381 to W.E.K. and NIRG-00-2335 to Y.W.), the National Institutes of Health (Grants AG01039 and AG20226 to W.E.K., AG18402 to C.A.M., and AG05133 to S.T.D.), and Institute for the Study of Aging/American Federation of Aging Research (Y.W.). We thank Dr. Bryan Roth (Case Western Reserve University) and Dr. Linda Brady [National Institute of Mental Health (NIMH)] for allowing us access to the NIMH Psychoactive Drug Screening Program. We thank Dr. Robert Sweet for his help in procuring the appropriate tissue samples and the families that made brain tissue available to the University of Pittsburgh Alzheimer Disease Research Center Brain Bank.

  • Correspondence should be addressed to Dr. William E. Klunk, University of Pittsburgh, 705 Parran Hall– GSPH, 130 DeSoto Street, Pittsburgh, PA 15213. E-mail: klunkwe{at}msx.upmc.edu.

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The Journal of Neuroscience: 23 (6)
Journal of Neuroscience
Vol. 23, Issue 6
15 Mar 2003
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The Binding of 2-(4′-Methylaminophenyl)Benzothiazole to Postmortem Brain Homogenates Is Dominated by the Amyloid Component
William E. Klunk, Yanming Wang, Guo-feng Huang, Manik L. Debnath, Daniel P. Holt, Li Shao, Ronald L. Hamilton, Milos D. Ikonomovic, Steven T. DeKosky, Chester A. Mathis
Journal of Neuroscience 15 March 2003, 23 (6) 2086-2092; DOI: 10.1523/JNEUROSCI.23-06-02086.2003

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The Binding of 2-(4′-Methylaminophenyl)Benzothiazole to Postmortem Brain Homogenates Is Dominated by the Amyloid Component
William E. Klunk, Yanming Wang, Guo-feng Huang, Manik L. Debnath, Daniel P. Holt, Li Shao, Ronald L. Hamilton, Milos D. Ikonomovic, Steven T. DeKosky, Chester A. Mathis
Journal of Neuroscience 15 March 2003, 23 (6) 2086-2092; DOI: 10.1523/JNEUROSCI.23-06-02086.2003
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Keywords

  • Alzheimer's disease
  • neuroimaging
  • plaques
  • neurofibrillary tangles
  • positron emission tomography
  • PET
  • thioflavin-T
  • benzothiazole
  • postmortem
  • binding

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