Article Figures & Data
Figures
- Fig. 1.
Quantitative evaluation of proximal and distal Purkinje cell dendritic spines innervated by climbing fiber (CF) terminals and by parallel fiber (PF) varicosities and their GluRδ2 expression in the TTX experimental group. Immunogold particle counts were done after 7 d of infusion with vehicle (v) and TTX and at 45 and 135 d after TTX removal. a, Average number ± SD of CF synapses per unit area of 34,445 μm2. b, c, Gold particle density per micrometer of postsynaptic density length inCF- and PF-innervated spines. The total number of climbing fiber-innervated spines (black columns) drops under TTX and recovers at 45 and 135 d (a). The number of GluRδ2-labeled synapses (gray columns) is a small fraction in vehicle-treated animals (a), and they have a very small gold particle density (b), whereas under TTX they represent a high fraction (a) and have a high gold particle density (b). Parallel fiber synapses on the spiny branchlets maintain similar values of high GluRδ2 density in all conditions (c).
- Fig. 2.
GluRδ2 expression in TTX-treated cerebella.a–c, Climbing fiber-innervated spines. GluRδ2 expression is virtually absent in vehicle-treated cerebella (a) and after 135 d of recovery (c), whereas it is highly expressed at 7 d of TTX treatment (b). d–f, Parallel fiber-innervated spines emerging from the branchlets. GluRδ2 expression is high in all three conditions. Scale bar, 0.23 μm.
- Fig. 3.
Serial reconstruction of a spine (asterisk) free of presynaptic terminal that emerges from the proximal dendrites of a Purkinje cell at 6 d from a subtotal lesion of the inferior olive. a–e, Consecutive sections showing the entire spine profile with no presynaptic terminals. Note that in d (arrowheads) there is a thickening of the postsynaptic membrane. Scale bar, 0.36 μm.
- Fig. 4.
Quantitative evaluation of proximal dendritic spines innervated by climbing fiber (CF) and distal dendritic spines innervated by parallel fiber (PF) and their GluRδ2 expression in rats with inferior olive lesion at different periods in days. a, Percentage of climbing fiber-innervated spines bearing GluRδ2. The percentage value increases remarkably up to 90 d after the lesion and then recovers at 150 d and is similar to control (c) at 270 d. b,c, Number of gold particles per micrometer of postsynaptic density length in climbing fiber- and parallel fiber-innervated spines. The density in the climbing fiber-innervated spines is increased up to 90 d and then recovers at 150 d and is similar to control at 270 d, whereas the density in the parallel fiber-innervated spines is normal in all conditions.
- Fig. 5.
GluRδ2 expression after a subtotal lesion of the inferior olive. a–d, Climbing fiber-innervated spine in control (a) and at 6 (b), 90 (c), and 270 (d) d after the lesion. A high density of GluRδ2s is present in band c and not in a and d.e–h, Parallel fiber-innervated spines emerging from the branchlets at the same periods after lesion are shown. A high density of GluRδ2s is visible in all conditions. Scale bar, 0.23 μm.
- Fig. 6.
Dark degenerating climbing fiber terminal innervating three spines at 6 d after subtotal lesion of the inferior olive. Note the high expression of GluRδ2. Scale bar, 0.18 μm.
