Figure 1.
Expression of Barhl1 in developing cerebellar and precerebellar systems. A, The Barhl1 transcript was detected by in situ hybridization in a whole-mount embryo at E11.5. B, C, Localization of Barhl1 transcripts in sagittal (B) and coronal (C) sections through brains of E14.5 embryos. D,E, Neurons within the reticulotegmental and lateral reticular nuclei in coronal sections through brains of P0 mice were immunolabeled with an anti-Barhl1 antibody. F–H, Localization of Barhl1 transcripts in coronal sections through brains of P2 (F) and P8 (G, H) animals. Barhl1 expression is seen in restricted areas within the diencephalon, mesencephalon, cerebellum, brainstem, and neural tube. Note the strong expression of Barhl1 in the rhombic lip, external granule layer, anterior and posterior extramural migratory streams, and precerebellar nuclei extending mossy fibers (A–H), but not in the inferior olivary neurons (C). aes, Anterior extramural migratory stream; ARL, anterior rhombic lip; Cb, cerebellum; ECN, external cuneate nucleus; EGL, external granule layer; IC, inferior colliculus; IGL, internal granule layer; ION, inferior olivary nucleus; LRN, lateral reticular nucleus; M, mesencephalon; Me; medulla; MM, mammillary region; NT, neural tube; pes, posterior extramural migratory stream; PGN, pontine gray nucleus; PRL, posterior rhombic lip; R, rhombencephalon; RTN, reticulotegmental nucleus. Scale bar: E, 25 μm; D, G, 50 μm; F, H, 100 μm; B, C, 250 μm; A, 385 μm.