Article Figures & Data
Figures
- Figure 1.
CD4+ T cells reactive to non-CNS antigen fail to support FMN survival. a–d, Thionin-stained FMNs of the control and axotomized facial motor nuclei 4 weeks after facial nerve axotomy. a, WT mice without reconstitution (none); inset shows a thionin-labeled FMN with clear nucleus used for counting. b, RAG-2 KO without reconstitution (none). c, WT-derived CD4+ T cell-reconstituted (WT-CD4+ T) RAG-2 KO. d, DO11.10-derived CD4+ T cell-reconstituted (DO11.10 CD4+ T) RAG-2 KO. e, Average percentage survival ± SEM of FMNs from right (axotomized) side of WT (black), RAG-2 KO (gray), WT-derived CD4+ T cell-reconstituted RAG-2 KO, and DO11.10-derived CD4+ T cell-reconstituted RAG-2KO mice, relative to the unoperated left-side controls [the asterisk and the number sign represent a significant difference from WT (none) and RAG-2KO (none), respectively, at p < 0.01; n = 6 for all groups].
- Figure 2.
Immunohistochemistry of MAC-1-labeled microglia shows that microglia are present and reactive in MHC II KO mice. MAC-1-labeled microglia in the control and axotomized facial motor nuclei 7 d after facial nerve transection. a, Control side of WT, MHC II KO, and RAG-2 KO mice (4× and 40× magnification). b, Axotomized side of WT, MHCII KO, and RAG-2 KO mice (4× and 40× magnification). Arrows indicate phagocytic cluster.
- Figure 3.
FMN survival requires both peripheral and central APC function. Thionin-stained FMNs of the control and axotomized facial motor nuclei 4 weeks after facial nerve axotomy. a, WT mice without reconstitution (none). b, MHC II KO chimera mice without reconstitution (none). c, Naive CD4+ T cell-reconstituted MHC II KO chimera mice. d, Activated CD4+ T cell reconstituted MHC II KO chimera mice. e, WT chimera without reconstitution (none). f, Naive CD4+ T cell reconstituted WT chimera mice. g, Activated CD4+ T cell-reconstituted WT chimera mice.
- Figure 4.
FMN survival requires both peripheral and central APC function. Average percentage survival ± SEM of FMNs from right [axotomized (Ax)] side of WT (black), MHC II KO chimera (gray), and WT chimera (hatched) mice either without reconstitution (–) or with naive or activated CD4+ T cell reconstitution (+) relative to the unoperated left-side controls (C) (points represent individual animals in each group, and asterisks represent a significant difference at p < 0.001; n = 6–9 for all groups).
Additional Files
Supplemental data
Supplementary Figure
Files in this Data Supplement:
- Supplementary Fig. 1 - Supplemental Figure 1. Experimental design for BM chimera studies. WT and MHC II KO mice were subjected to a lethal dose of whole-body irradiation and subsequent BM transplant. MHC II KO mice received WT-derived BM, resulting in an MHC II KO-chimera containing MHC II-negative resident microglial cells and MHC II-positive BM-derived APCs. WT mice received MHC II KO-derived BM, resulting in a WT chimera containing MHC II-positive resident microglial cells and MHC II-negative BM-derived APCs. One week before facial nerve injury, each chimera model was reconstituted with naive CD4+ T cells (from the lymph nodes of WT mice), activated CD4+ T cells (from cervical lymph nodes of WT mice 3 d after facial nerve axotomy), or no reconstitution (none).
