Article Figures & Data
Figures
- Figure 1.
Localization of p75NTR in the normal and injured spinal cord. A, Cross-section of normal spinal cord. The arrow indicates p75NTR immunoreactivity in the dorsal column, and the arrowhead indicates the absence of p75NTR immunoreactivity in the CST. B, Cross-section 1 mm caudal to the lesion site 3 d after injury. The arrow indicates p75NTR immunoreactivity in the dorsal column, the arrowhead indicates the absence of p75NTR stain in the CST, and the asterisk indicates a cavity caused by injury. C, Sagittal spinal cord section 3 d after injury. The arrowhead indicates p75NTR immunoreactivity in the lesion site, and the double arrowheads indicate reduction in p75NTR immunoreactivity rostral to the lesion site. R, Rostral. Scale bars, 100 μm.
- Figure 2.
Anterograde labeling of corticospinal fibers in the injured spinal cord of p75NTR-/- and control mice. A, B, T1 cross-sections of spinal cord from control mice and p75NTR-/- mice, respectively. C, D, Cross-sections of spinal cord 0.5 mm caudal to the lesion site from control mice and p75NTR-/- mice, respectively. Numerous corticospinal axons were labeled in the rostral segment (T1), but no axons were detected in the caudal sections. E, Quantification of the axon regeneration in wild-type controls and p75NTR-/- mice. No statistically significant difference in axon index at any location was found between mutant mice and control animals. Scale bar, 100 μm. The arrows show BDA-labeled CST axons in their normal position. Arrowheads show the absence of BDA-labeled CST axons.
- Figure 3.
Anterograde labeling of dorsal column fibers in the injured spinal cord of p75NTR-/- and control mice. A, B, Sagittal sections of the injured spinal cord from p75NTR-/- and control mice, respectively. FR-labeled axons stopped before the lesion site in both types of mice. C, Quantification of the dorsal column axons in wild-type controls and p75NTR-/- mice. No statistically significant difference in the axon index at any location was found between mutant and control groups. The arrows show the lesion site, and arrowheads show axons stopped at the lesion site. Scale bars, 100 μm. R, Rostral to the lesion site.
- Figure 4.
Anterograde labeling of dorsal column fibers in the injured spinal cord of mice treated with p75NTR-Fc or normal human IgG. A, B, Sagittal sections of the injured spinal cord from mice treated with p75NTR-Fc or normal human IgG, respectively. C, An enlarged view from the field in A as marked by a square and an arrow. D, An enlarged view from the field in B as marked by a square and an arrow. E, Quantification of the dorsal column axons in mice treated with p75NTR-Fc or normal human IgG. No statistically significant difference in the axon index at any location was found between the two groups of animals. The arrows show lesion site, and arrowheads show axons stopped abruptly. Scale bars, 100 μm (the bar in A also applies to B; the bar in C also applies to D). R, Rostral to the lesion site.
