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Exclusive Postsynaptic Action of Hypocretin-Orexin on Sublayer 6b Cortical Neurons

Laurence Bayer, Mauro Serafin, Emmanuel Eggermann, Benoît Saint-Mleux, Danièle Machard, Barbara E. Jones and Michel Mühlethaler
Journal of Neuroscience 28 July 2004, 24 (30) 6760-6764; https://doi.org/10.1523/JNEUROSCI.1783-04.2004
Laurence Bayer
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Mauro Serafin
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Emmanuel Eggermann
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Benoît Saint-Mleux
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Danièle Machard
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Barbara E. Jones
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Michel Mühlethaler
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Abstract

The hypocretin-orexin (hcrt-orx) neurons are thought to maintain wakefulness because their loss results in narcolepsy. This role may be fulfilled by the excitatory action that the hcrt-orx peptide exerts on multiple brainstem and forebrain systems that, in turn, promote cortical activation. Here, we examined whether hcrt-orx may also exert a postsynaptic excitatory action at the level of the cortex, where hcrt-orx fibers project. However, we found that neurons in layers 2-5 in the primary somatosensory cortex (SSp) were unresponsive to hcrt-orx. We then found that although all neurons tested in sublayer 6a were also unresponsive to hcrt-oxr, all those tested in sublayer 6b were highly sensitive to the peptide. The sublayer selectivity of hcrt-oxr was not restricted to the somatosensory cortex, because it was also found to be present in the primary visual cortex, the motor cortex, and the cingulate cortex. In the SSp, in which the hcrt-oxr effect was investigated further, it was demonstrated to be postsynaptic, to result from an interaction with Hcrtr2-OX2 receptors and to depend on the closure of a potassium conductance. Similar to the selectivity of action in the thalamus, where hcrt-oxr excites the nonspecific thalamocortical projection neurons and not the specific sensory relay neurons, here in the cortex, it excites a specific subset of cortical neurons which, through corticocortical projections, may also be involved in promoting widespread cortical activation.

  • arousal
  • cortex
  • hypocretin
  • orexin
  • sleep
  • waking

Introduction

The neurons of the lateral hypothalamus and perifornical area that express hypocretin-orexin (hcrt-oxr) (de Lecea et al., 1998; Sakurai et al., 1998) are thought to play a major role in promoting the state of wakefulness (for review, see Siegel et al., 2001; Beuckmann and Yanagisawa, 2002; Sutcliffe and de Lecea, 2002; Taheri et al., 2002). Indeed, various conditions that interfere with the integrity of the neurons, the peptides they secrete, or the receptors to the peptides are associated with a deficiency in wakefulness and the development of narcolepsy (Chemelli et al., 1999; Lin et al., 1999; Nishino et al., 2000; Peyron et al., 2000; Thannickal et al., 2000; Hara et al., 2001). The hcrt-oxr neurons synthesize two peptides (hypocretin 1 and hypocretin 2; also known as orexin A and B) that act on two types of receptors (OX1 and OX2; also known as Hcrtr1 and Hcrtr2) (Sakurai et al., 1998). These receptors are expressed widely throughout the CNS, including the cerebral cortex (Lu et al., 2000; Marcus et al., 2001), to where the hcrt-oxr fibers also project (Peyron et al., 1998; van den Pol, 1999). Hypocretin-orexin receptors and fibers are particularly densely distributed in areas known to be involved in promoting wakefulness by stimulating cortical activation (for review, see Steriade and McCarley, 1990; McCormick and Bal, 1997; Jones, 2000, 2003; Steriade, 2000). Hcrt-oxr peptides have been shown to exert excitatory actions on many of these systems, including noradrenergic locus coeruleus neurons, histaminergic neurons, and cholinergic mesopontine and basal forebrain neurons (Hagan et al., 1999; Horvath et al., 1999; Bourgin et al., 2000; Ivanov and Aston-Jones, 2000; Methippara et al., 2000; Bayer et al., 2001; Eggermann et al., 2001; Eriksson et al., 2001; Huang et al., 2001; Xi et al., 2001; Brown et al., 2002; Burlet et al., 2002). In a recent study, we have shown that thalamic neurons of the intralaminar and midline nuclei, which comprise the nonspecific thalamocortical projection system, and not those of the specific relay nuclei, are also excited by hcrt-oxr (Bayer et al., 2002). Interestingly, hcrt-oxr has an excitatory effect on the terminals of those same thalamic neurons in the cortex (Lambe and Aghajanian, 2003). In the present study, we examined whether hcrt-oxr may also excite cortical neurons by a direct postsynaptic action. Using cortical slices from different regions, we tested the effect of the hcrt-oxr peptides on neurons in different layers of the primary somatosensory cortex (SSp), the primary visual cortex (VISp), the primary motor cortex (MOp), and the cingulate cortex (CG).

Materials and Methods

Electrophysiological recordings. Brain slices were obtained from young rats (15-25 d of age) reared at the animal facility of the Geneva Medical Centre and treated according to the regulations of the Swiss Federal Veterinary Office. Coronal slices (250-300 μm thick) that included the cerebral cortex (Swanson, 1992) were incubated at room temperature in artificial CSF (ACSF), which contained (in mm): 130 NaCl, 5 KCl, 1.25 KH2PO4, 1.3 MgSO4, 20 NaHCO3, 10 glucose, and 2.4 CaCl2, bubbled with a mixture of 95% O2 and 5% CO2. Experiments in which EK was changed were done with the following ACSF (in mm): 124.25 NaCl, 10.75 KCl, 1.25 KH2PO4, 1.3 MgSO4, 20 NaHCO3, 10 glucose, and 2.4 CaCl2. According to the Nernst equation [EK = 2.303 (RT/zF) · log (Ko/Ki)] was -77.8 mV in the control ACSF (Ko = 6.25 mm) and -61.0 mV in the modified ACSF (Ko = 12 mm). Individual slices were transferred to a thermoregulated (32°C) chamber on a Zeiss (Oberkochen, Germany) Axioskop equipped with an infrared camera (Dodt and Zieglgansberger, 1994). Slices were maintained immersed and continuously superfused at 3-5 ml/min. Patch electrodes were pulled on a DMZ universal puller (Zeitz-Instrumente, Münich, Germany) from borosilicate glass capillaries (GC150F-10; Clark Electromedical Instruments, Pangbourne, UK). The pipettes (5-10 MΩ) contained the following solution (in mm): 126 KMeSO4, 8 phosphocreatine, 4 KCl, 5 MgCl2, 10 HEPES, 3 Na2ATP, 0.1 GTP, and 0.1 BAPTA, pH 7.4 (290-310 mOsm). Neurobiotin (0.2%; Vector Laboratories, Burlingame, CA) was added to the intrapipette solution when needed. Current-clamp and voltage-clamp recordings were obtained in the whole-cell configuration using an Axopatch 200 B and pClamp 8.0 software (Axon Instruments, Foster City, CA). Liquid junction potential (-9 mV) was calculated using JPCalc (bundled in the Axon software). Current-voltage plots (I-V curves) were obtained in the presence of tetrodotoxin (TTX; 1 μm),(+)-5-methyl-10,11-dihydro-5H-dibenzo [a,d] cyclohepten-5,10-imine maleate (20 μm), and APV (50 μm) before and during hcrt2-orxB application using slow (6 sec) ramps between +20 and -120 mV. Ramps were generated and analyzed using pClamp 8.0 software.

Hcrt-orx (Bachem, Bubendorf, Switzerland) peptides were tested by dissolving them at the proper concentration in the perfusion solution. Input resistance was occasionally monitored throughout the pharmacological experiments using short-lasting hyperpolarizing current pulses. At the maximum of the depolarizing effect of the hcrt-oxr, the membrane potential was clamped manually back to its resting value, thus allowing evaluation of the change in membrane resistance.

Histology. After electrophysiological recordings, slices were fixed in an ice-cold solution containing 3% paraformaldehyde. Neurobiotin-filled neurons were then visualized using the avidin-biotinylated horseradish peroxydase complex reaction (Vectastain, ABC Elite kit; Vector Laboratories) with 3-3′-diaminobenzidine (Sigma, St. Louis, MO) as a chromogen. To distinguish the location of stained cells within cortical layers, slices were counterstained with 0.3% of toluidin blue. Photomicrographs were realized with a digital microscope camera (Axiocam; Zeiss) and printed with Photoshop 6.0 (Adobe Systems, San Jose, CA).

Results

Neurons of cortical sublayer 6b of the SSp are selectively exited by hcrt-oxr

Cortical neurons of the SSp (Fig. 1A) were visualized by infrared video-microscopy and tested for their sensitivity to bath application of hcrt-oxr (10-500 nm). As illustrated in Figure 1B-D, we found most cortical neurons of layers 2-5 to be unresponsive to hcrt-oxr (as judged by monitoring membrane potential, membrane resistance, and changes in the occurrence of synaptic potentials). To ensure the validity of these negative results, most studies were conducted with orxA, which is as potent as orx B on Hcrt2-OX2 receptors but more active than orxB on Hcrt1-OX1 receptors (Sakurai et al., 1998). Starting from the surface, we found, as illustrated in Figure 1, B1 and B2, that 21 of 21 cells estimated to be in layers 2-3 were unresponsive (nine tested with orxA at 100 nm, four with orxA at 500 nm, and eight with orxB at 100 nm). In layers 4-5 (Fig. 1C1,C2), 28 of 31 cells showed no response to hcrt-oxr (20 tested with orxA at 100 nm, 5 with orxA at 500 nm, and 3 with orxB at 100 nm). In 3 of 31 cells of layer 4/5, a minute depolarization was found that resulted from an increase in spontaneous synaptic potentials (Fig. 1G, middle panel). The synaptic potentials and depolarization were eliminated by either TTX or in modified ACSF (0.1 mm Ca2+/10 mm Mg2+) known to block synaptic transmission (n = 2 of 2) (Fig. 1G, right panel).

  Figure 1.
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Figure 1.

Exclusive action of hcrt-orx on cortical neurons of sublayer 6b in the SSp. A, Toluidin blue counterstained cortical slice slab containing two recorded neurons (arrowheads) labeled with neurobiotin in sublayers 6a and 6b (separated by a horizontal band of fibers; *) and the responses of which to hcrt-oxr are shown in D and E. B1, Absence of response to hcrt-oxr of a neuron in layer 2/3. B2, Summarized results showing that all neurons of layer 2/3 were unresponsive to hcrt-oxr. n represents the total number of cells tested. C1, Absence of response to hcrt-oxr of a neuron in layer 4/5. C2, Summarized results showing that most neurons of layer 2/3 were unresponsive to hcrt-oxr (see Results). D1, Absence of response to hcrt-oxr of a neuron in layer 6a. D2, Summarized results showing that most neurons of layer 6a were unresponsive to hcrt-oxr (see Results). E1, Depolarizing response to hcrt-oxr of a neuron in layer 6b. E2, Summarized results showing that all neurons of layer 6b were depolarized by hcrt-oxr. F1, F2, Enlargement of neurons 1 and 2 from A. Scale bar, 15 μm. G, Increase in PSPs in a layer 5 neuron (middle panel) is impeded in the presence of a low calcium-high magnesium ACSF. Calibration: 5 mV, 2 sec. CC, Corpus callosum.

In contrast to the negative results in layers 2-5, neurons responsive to hcrt-oxr were found in layer 6. However, even within that layer, the responsive cells were limited in their distribution. Indeed, as evident under infrared video-microscopy, all of the responsive cells were located within layer 6b, which is separated from layer 6a by a thin horizontal band of fibers (Fig. 1A, asterisk). In layer 6a (Fig. 1D1,D2) eight of nine neurons tested were insensitive to hcrt-oxr (two tested with orxA at 100 nm, and six with orxB at 100 nm). One neuron was slightly depolarized by orxB at 100 nm. Two unresponsive cells were injected with neurobiotin and confirmed by histochemistry to be located in sublayer 6a (Fig. 1F1, from cell 1 in A). In contrast (Fig. 1E1,E2), all cells (37 of 37) tested in sublayer 6b were excited by hcrt-oxr (27 tested with 100 nm orxB, 9 with 500 nm orxB, and 1 with 100 nm orxA). Eight cells depolarized by hcrt-oxr and visualized in sublayer 6b were labeled with neurobiotin and confirmed to be localized within that sublayer (Fig. 1F2, from cell 2 in A).

Hcrt-orx excitation of sublayer 6b neurons in the SSp cortex is postsynaptic and involves Hcrtr2-OX2 receptors

The hcrt-orx depolarization was further characterized in SSp cortical neurons of sublayer 6b. It was found that the effect persists in the presence of either TTX (1 μm; n = 4 of 4) (Fig. 2A) or in a modified ACSF (0.1 mm Ca2+/10 mm Mg2+; n = 3 of 3) (Fig. 2B) known to block synaptic transmission, thus indicating that the hcrt-orx action is postsynaptic. As illustrated in Figure 2C, the effects of hcrt-orx were dose dependent with a threshold above 10 nm for both hcrt1-orxA and hcrt2-orxB. We then explored the receptors involved in the depolarizing action of hcrt-orx by comparing the effect of hcrt1-orxA with that of hcrt2-orxB (Fig. 2D), both applied at 30 nm, and found that the effects did not differ statistically (mean depolarization ± SEM for hcrt1-orxA = 9.2 ± 1.67 mV and for hcrt2-orxB = 10.3 ± 1.88 mV; n = 5; paired t test, t = -0.436; p = 0.674). Together, these results suggest that the hcrt-orx effect is caused by an action on Hcrtr2-OX2 receptors (Sakurai et al., 1998).

  Figure 2.
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Figure 2.

Mechanism of action of hcrt-oxr on SSp sublayer 6b cortical neurons. A, B, Response to hcrt-oxr in the presence of TTX at 1 μm (A) or in the presence of a modified ACSF containing 0.1 mm Ca2+ and 10 mm Mg2+ (B). C, Dose-responses to hcrt2-oxrB applied between 10 and 50 nm. D, Comparison of the depolarization induced by hcrt1-oxrA and hcrt2-oxrB (applied at 30 nm).

Hcrt-orx depolarization of sublayer 6b neurons in the SSp is attributable to the blocking of a potassium current

The depolarization by hcrt-orx was accompanied by a small increase in membrane input resistance (nine of nine cells) (Fig. 2B, asterisk). To test whether such an effect could result from the closure of a potassium conductance, we turned to voltage-clamp studies. In an ACSF with Ko at 6.25 mm (estimated EK = -77.8 mV), comparing voltage-clamp ramps in control and in the presence of hcrt2-orxB (Fig. 3A, C, subtraction 1-2) indicated a reversal of the hcrt-orx effect of approximately -80 mV. In five cells (Fig. 3C, inset), the mean (±SEM) reversal was -77.4 ± 1.14 mV, thus very close to the estimated EK. Performing the same experiment (Fig. 3B, C, subtraction 3-4) in an ACSF with Ko at 12 mm (estimated EK = -61.0 mV) indicated a reversal of the hcrt-orx effect of approximately -60 mV. In five cells (Fig. 3C, inset), the mean reversal was now -60.4 ± 0.68 mV, thus again very close to the estimated EK. These data thus indicate that the hcrt-orx effect in the SSp is mediated through the closure of a potassium conductance.

  Figure 3.
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Figure 3.

Hcrt-oxr blocks a K current. A, Voltage-clamp ramps in absence (1) or presence (2) of hcrt-oxr with Ko at 6.25 mm. B, Voltage-clamp ramps in absence (3) or presence (4) of hcrt-oxr with Ko at 12.0 mm. C, Subtractions of curves from A (1, 2) and B (3, 4). Inset, Reversal potentials for both conditions with respect to Nernst relationship (see Materials and Methods).

Selective action of hcrt-orx in other cortical areas

We subsequently investigated whether the selectivity of the hcrt-orx effects to layer 6b in the SSp was also present in other cortical areas. Thus, we first tested hcrt-orx on cortical neurons in another sensory field, the VISp, and found that in cortical layers 2-6a, no cells (27 of 29) were directly depolarized by either orxA (11 of 11 at 100 nm, and 9 of 9 at 500nm) or orxB (7 of 7 at 100 nm). As in the SSp, however, a small minority of cells (2 of 29) showed an increase in spontaneous synaptic potentials (in that case, in layer 6a, with 100 nm of orxB). In contrast, all neurons of sublayer 6b were depolarized by hcrt-orx (five of five tested with orxB at 100 nm) (Fig. 4A).

  Figure 4.
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Figure 4.

Exclusive action of hcrt-oxr in sublayer 6b in primary visual cortex, primary motor cortex, and cingulate cortex. A, B, Excitatory action of hcrt-oxr on sublayer 6b neurons of the primary visual cortex (A), primary motor cortex (B1), and cingulate cortex (B2). Scale bar, 15 μm (in all insets showing infrared images of recorded cells). AQ, Aqueduct; CP, caudate-putamen; DG, dentate gyrus; LS, lateral septum; LV, lateral ventricle; PAG, periacqueductal gray.

Next, we tested hcrt-orx on neurons of another type of cortex, the MOp, and found similar results with no cells (29 of 29) in layers 2-6a being sensitive to either orxA (16 of 16 at 100 nm, and 5 of 5 at 500 nm) or orxB (8 of 8 at 100 nm), whereas all cells in sublayer 6b were depolarized (four of four with orxB at 100 nm) (Fig. 4B1).

Finally, we tested hcrt-orx in a nonmotor, nonsensory cortex, the CG, and again found similar results with 38 of 39 cells in layers 2-6a being insensitive to either orxA (13 of 13 at 100 nm,and 9 of 9 at 500 nm) or orxB (16 of 16 at 100 nm). One cell of 39 showed an increase in spontaneous synaptic potentials. As in the other cortices, all cells in sublayer 6b were depolarized by hcrt-orx (four of four with orxB at 100 nm) (Fig. 4B2).

Discussion

The present study demonstrates that in all cortical regions tested, neurons showed excitatory postsynaptic responses to hcrt-oxr. However, these responses occurred exclusively in neurons of sublayer 6b. As studied in detail within the SSp, the excitatory effect was found to be mediated by Hcrtr2-OX2 receptors and caused by the closure of a potassium conductance.

Many studies have shown that hcrt-oxr exerts an excitatory action on CNS neurons (for review, see Sutcliffe and de Lecea, 2002). The receptors mediating these actions vary across cell groups but are commonly of the Hcrtr2-OX2 subtype within the subcortical arousal systems (Eriksson et al., 2001; Bayer et al., 2002; Brown et al., 2002; Burlet et al., 2002), except those on locus coeruleus neurons, which are of the Hcrtr1-OX1 subtype (Bourgin et al., 2000). Here in the cortical neurons of layer 6b, the equivalent effect of hcrt1-oxrA and hcrt2-oxrB also suggests the effect is mediated by Hcrtr2-OX2 receptors (Sakurai et al., 1998). This assumption is supported by histochemical evidence showing a higher density of Hcrtr2-OX2 than Hcrtr1-OX1 receptors in the cortex and a higher density of the Hcrtr2-OX2 receptors in the deep than in the superficial layers of the cortex (Marcus et al., 2001). The excitatory action mediated by hcrt-oxr receptors has been shown to involve several mechanisms, including an electrogenic pump and calcium current in histaminergic neurons (Eriksson et al., 2001) and a nonselective cation conductance in mesopontine cholinergic (Burlet et al., 2002) and serotoninergic dorsal raphe neurons (Brown et al., 2002). As was the case in thalamic neurons of the diffuse thalamocortical projection system (Bayer et al., 2002), we found here that the depolarization induced by hcrt-oxr in the cortical neurons was attributable to block of a potassium conductance.

The excitatory action of hcrt-oxr on subcortical arousal systems, including the noradrenergic, histaminergic, cholinergic mesopontine, and basal forebrain cell groups has been emphasized as the principal way in which this peptide may promote behavioral arousal and cortical activation to maintain wakefulness (Hagan et al., 1999; Horvath et al., 1999; Bourgin et al., 2000; Ivanov and Aston-Jones, 2000; Methippara et al., 2000; Bayer et al., 2001; Eggermann et al., 2001; Eriksson et al., 2001; Huang et al., 2001; Xi et al., 2001; Brown et al., 2002; Burlet et al., 2002). In addition, we demonstrated an excitatory action on the thalamic neurons of the nonspecific thalamocortical projection system (Bayer et al., 2002), which evokes widespread cortical activation (Steriade, 1981), and Lambe and Aghajanian (2003) demonstrated an excitatory presynaptic action on the terminals of that system in the cortex. Here, we show a direct postsynaptic excitatory effect of hcrt-oxr on cortical neurons. Interestingly, however, this effect is highly restricted to a small sublayer of cells, layer 6b. These data are in agreement with the little number of responsive cortical neurons as reported in cultures (van den Pol et al., 1998). Comparable with neurons of the nonspecific thalamocortical projection system, neurons in sublayer 6b project in a widespread manner to the cortical mantle (Clancy and Cauller, 1999; Reep, 2000). Indeed, they send corticocortical projections particularly to layer 1, which also receives input from nonspecific thalamocortical projection systems (Herkenham, 1986) and through which neurons in deeper cortical layers, including pyramidal cells, can be strongly influenced. Hcrt-oxr could thus stimulate widespread cortical activation by direct actions on sublayer 6b cortical neurons. The hcrt-oxrinergic system would thus exert a powerful activating influence on the cortex by direct excitation of this corticocortical system, in parallel with its excitation of the nonspecific thalamocortical projection system and the cholinergic basalocortical and other subcortical arousal systems.

Footnotes

  • This work was supported by grants from the Swiss Fonds National, Novartis, OTT, de Reuter, Roche, Schmidheiny Foundations (M.M., M.S.), the Canadian Medical Research Council (B.E.J.), and a Roche fellowship (L.B.).

  • Correspondence should be addressed to Dr. M. Mühlethaler, Centre Médical Universitaire Département de Physiologie, 1 Rue Michel-Servet, 1211 Genève 4, Switzerland. E-mail: michel.muhlethaler{at}medecine.unige.ch.

  • Copyright © 2004 Society for Neuroscience 0270-6474/04/246760-05$15.00/0

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The Journal of Neuroscience: 24 (30)
Journal of Neuroscience
Vol. 24, Issue 30
28 Jul 2004
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Exclusive Postsynaptic Action of Hypocretin-Orexin on Sublayer 6b Cortical Neurons
Laurence Bayer, Mauro Serafin, Emmanuel Eggermann, Benoît Saint-Mleux, Danièle Machard, Barbara E. Jones, Michel Mühlethaler
Journal of Neuroscience 28 July 2004, 24 (30) 6760-6764; DOI: 10.1523/JNEUROSCI.1783-04.2004

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Exclusive Postsynaptic Action of Hypocretin-Orexin on Sublayer 6b Cortical Neurons
Laurence Bayer, Mauro Serafin, Emmanuel Eggermann, Benoît Saint-Mleux, Danièle Machard, Barbara E. Jones, Michel Mühlethaler
Journal of Neuroscience 28 July 2004, 24 (30) 6760-6764; DOI: 10.1523/JNEUROSCI.1783-04.2004
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