Figure 5.
Whole-cell voltage-clamp recordings of mIPSCs from representative CeA neurons in the presence of the following (in μm): 1 TTX, 30 APV, 10 CNQX, and 1 CGP 55845A. A, Current traces from a naive rat. Acute superfusion of 44 mm ethanol onto this cell increased the frequency and amplitude of the mIPSCs, with recovery on washout (Wash). Subsequent superfusion of 30 μm bicuculline totally blocked these mIPSCs. B, Traces of mIPSCs from a CeA neuron of a CET rat. A 44 mm concentration of ethanol increased the frequency and amplitude of the mIPSCs, with recovery on washout. C, Cumulative frequency histogram for the naive neuron of A showing a shift to the left, indicating a shorter interevent interval (higher frequencies) during the application of 44 mm ethanol. D, Cumulative frequency histogram from the neuron of B showing a significant increase in mIPSC frequency. Data shown are means from a 2 min recording. E, Cumulative amplitude histogram from the neuron of A showing an increase in the distribution of mIPSC amplitudes. Note the shift to the right (especially in the first part of the curve, 1-10 pA) and elongation (beyond 15 pA) of the ethanol curve, compared with the control curve.F, Cumulative amplitude histogram from the neuron of B showing an increase in the distribution of mIPSC amplitude (note elongation of the ethanol curve beyond 30-50 pA). Prob, Probability.