Figure 1.
Effect of PKA inhibition on cAMP-dependent neurite outgrowth. Dissociated retinal neurons from E18 rats were plated in serum-free media on LN-1 or LN-2 in the presence or absence of FSK/IBMX, allowed to grow overnight, and were then fixed, stained for neurofilament with mAb RT97, and photographed. A, B, Control cultures on LN-1 (A) and LN-2 (B) are shown. Cultures in C and D were plated on LN-1 in the presence of FSK/IBMX; the culture in D was further treated with a mixture of PKA inhibitors (H89, 4 μm; PKI, 4 μm; RpcAMPS, 500 μm). Scale bar (in D), 50 μm.