Figure 4.
Detection of KARAP/DAP12 in microglial cells but not in astrocytes or oligodendrocytes. Cultures were labeled with anti-DAP12 antibody (red; A1, B1, C1) and with anti-Iba1 (A2), anti-GFAP (B2), or anti-GalC (C2) antibodies to identify microglia, astrocytes, and oligodendrocytes, respectively. A3, B3, C3, Merges of DAPI (blue), KARAP/DAP12 (red), and Iba1, GFAP, or GalC (green) stainings. Arrowheads, KARAP/DAP12-positive cells; arrows, nuclei of KARAP/DAP12-negative cells. Scale bars (in A3, B3, C3), 5 μm.