Article Figures & Data
Figures
- Figure 2.
The histogram shows the numbers of microtransplanted DRG axon fragments proximal and distal to the lesion in DNA enzyme-treated and untreated spinal cords.
- Figure 1.
Confocal photomicrographs of TGF-β-stimulated primary astrocyte cultures treated with the DNA enzyme (A) or control DNA enzyme (B) or left untreated (C) and stained with the CS-56 antibody. Uptake of the biotin-streptavidin-labeled DNA enzyme can be seen in the astrocytes (D). Scale bars, 10 μm. E, F, RT-PCR-Southern blot of primary astrocyte cultures for XT-1 (E) and RT-PCR for GAPDH and cyclophilin (F) of the same mRNA. G, Dot blot of primary astrocyte cultures treated with the DNA enzyme or control DNA enzyme and stained with the CS-56 antibody. H, Restaining of the same dot blot with GFAP. I, RT-PCR-Southern blot for the XT-1 in a DNA enzyme- and control DNA enzyme-treated spinal cord.
- Figure 3.
Confocal photomicrographs of treated and untreated spinal cords. Red/green (A) and green (A′) channels show the green DRGs and their axons passing the distinct borders of the lesion cores (red CS-56 staining) in a DNA enzyme-treated spinal cord. Axons that enter the lesion core become dystrophic (A, A′, arrows). Red/green (B) and green (B′) channels show an untreated spinal cord with the green DRG and red CS-56 staining. Note the struggling axon in the heart of the lesion. Scale bar, 50 μm. C, Diffusion of a labeled DNA enzyme in vivo 7 d after treatment. Scale bar, 100 μm. D, GFAP-stained section of the lesion area in a different animal. Note the disrupted astrocyte alignment in the core and borders of the lesion (L) and the aligned astrocytes (arrows) in the region where axons regenerate.
Tables
Name Sequence Length of end product Reference XT-1 XT1s1 316bp Goetting et al. (2000) 5′-CTTCATCCGCCTGGGTCTTCG-3′ XT1as1 5′-GTAGTGTGTGAATTCCGCAGTGG-3′ nested XT1s2 5′-GGGCTGAGTCATCGCTACACACG-3′ nested XT1as2 5′-CGATGACATTGACAGGATCCACC-3′ GAPDH GAPDHs 78bp Tajima et al. (1999) 5′-GAACATCATCCCTGCATACA-3′ GAPDHas 5′-CCAGTGAGCTTCCCGTTCA-3′ Cyclophilin cyclo as 65 bp Danielson et al. (1988) 5′-TGTGCCAGGGTGGTGACTT-3′ cyclo as 5′-TTTCTCTCCGTAGATGGACTT-3′
