Figure 3.
ORIS at multiple wavelengths. A-D, Spike-triggered image division at 546 ± 10 nm (A) (n = 208 spikes), 605 ± 10 nm (B) (n = 185 spikes), 630 ± 10 nm (C) (n = 218 spikes), and 700 ± 10 nm (D) (n = 215 spikes) demonstrate the wavelength dependence of the optical signal (see Discussion). E, Magnified example of spike-triggered image divisions at four wavelengths at 0.3 sec (early) after IISs. F, Magnified example of spike-triggered image divisions at four wavelengths at 2.5 sec (late) after IISs. G, The amplitude and time course of the intrinsic signal change was measured from a single ROI in the focus (dark blue) and multiple, symmetric ROIs in the surrounding cortex (red, orange, light blue, yellow). H, Inverted optical signals recorded from the surrounding cortex are smaller in amplitude than signals recorded from the focus, regardless of wavelength, and appear in all directions. The amplitude of the signals decreases with increasing wavelength. Scale bars, 1 mm