Figure 3.
Absence of NaV1.6 does not affect retinal morphology. a, Hematoxylin and eosin staining of P16 control and P16 Scn8admu (dmu) retinas does not reveal overt abnormalities. b, Quantification of the layer thickness. Error bars represent SEM. c, In Scn8admu mice, GFAP is restricted to the end feet of Müller cells, indicating the absence of reactive gliosis. d-f, A survey of the Scn8admu photoreceptor ultrastructure at P16 shows normal outer and inner segments with no signs of degeneration (d). e, f, In addition, in the outer plexiform layer, bipolar (B) and horizontal (H) dendritic processes invaginate rod spherules to form synaptic clefts that have the normal triad appearance with the electron-dense ribbon located between the horizontal cells. Scale bar: (in f) a, c, 60 μm; d, 3 μm; e, 0.5 μm; f, 0.2 μm. pe, Pigment epithelium; os, outer segment; is, inner segment; onl, outer nuclear layer; opl, outer plexiform layer; inl, inner nuclear layer; ipl, inner plexiform layer; gcl, ganglion cell layer; ofl, optic fiber layer.