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- supplemental material - Supplemental Figure 1. Co-immunoprecipitation of cytosolic sortilin (cytSort) with BDNF. HEK 293 cells were co-transfected with cDNA�s encoding a mutant cytoplasmic region sortilin fused with GFP, as described in Materials and Methods, along with carboxy terminal tagged BDNF-HA. Cell lysates were immunoprecipitated with HA antibodies and immunoblotted with GFP antibodies (top panel). Immunoprecipitation of HA-BDNF was confirmed by immunoblotting with HA antibodies (middle panel). Crude lysates were immunoblotted with GFP antibodies to confirm equivalent expression levels (bottom panel).
- supplemental material - Supplemental Figure 2. pH dependence of BDNF-sortilin interaction. HEK 293 cells were co-transfected with cDNA�s encoding sortilin and carboxy terminal HA-BDNFVal. Cell lysates were adjusted to the indicated pH�s, and immunoprecipitated with HA antibodies and immunoblotted with sortilin antibodies (top panel). Immunoprecipitation of BDNF-HA was confirmed by immunoblotting with HA antibodies (middle panel). Crude lysates were immunoblotted with sortilin antibodies to confirm equivalent expression levels (bottom panel).