Article Figures & Data
Figures
- Figure 1.
PDAPP mice on a C57BL/6 background exhibit profound spatial learning deficits from an early age. Performance was assessed by distance to find the visible platform (cued) versus the hidden platform (spatial positions 1-5) in the water maze. Each point represents the average of four daily trials. Significant main effects of genotype are indicated by *, and a significant genotype-by-day interaction is indicated by **. Error bars represent SEM.
- Figure 2.
Old mice of each genotype perform worse than young or middle-aged mice in the spatial version of the water maze. Each point represents the average of four daily trials. Significant main effects of age are indicated by *. Error bars represent SEM.
- Figure 3.
PDAPP mice developed more severe age-dependent spatial learning deficits than wild-type mice. Average swim distance across all five platform positions was assessed. Each bar represents the average performance across all platform positions. Significant main effects of genotype are indicated by **, and a significant genotype-by-age interaction is indicated by **. Error bars represent SEM.
- Figure 4.
Both Aβ (A) and thioflavine-S+ Aβ (B) load increased with age in PDAPP mice. Significant main effects of age are indicated by *. Error bars represent SEM.
- Figure 5.
Treatment with 10D5 improved the spatial learning performance of aged PDAPP mice. Each point represents the average of four daily trials. Significant main effects of age are indicated by *. Error bars represent SEM.
- Figure 6.
Treatment with 10D5 reduced Aβ (A) and thioflavine-S+ Aβ (B) load and increased Aβ40 (C) and Aβ42 (D) levels in the plasma of aged PDAPP mice. Significant main effects of age are indicated by *. Error bars represent SEM.
- Figure 7.
Treatment with 10 D5 improved hippocampal LTP in aged PDAPP mice. A, Six series of tetanic bursts consisting of six trains of six pulses were delivered at time 0 (arrows). At 60 min after tetani, PDAPP plus saline mice exhibited impaired LTP compared with PDAPP plus 10D5 and WT mice. B, The bars represent levels of LTP 60 min after the induction of tetani. Traces depict EPSPs before (dotted traces) and 60 min after (solid traces) tetanic bursts. Error bars represent SEM.
Tables
Assay Young Old p PDAPP carbonate-soluble Aβ40 0.29 ± 0.03 2.95 ± 0.67 0.0002 PDAPP carbonate-insoluble Aβ40 1.21 ± 0.15 67.23 ± 15.56 0.00008 PDAPP carbonate-soluble Aβ42 0.38 ± 0.05 14.97 ± 3.17 0.00003 PDAPP carbonate-insoluble Aβ42 4.81 ± 0.65 667.89 ± 143.61 0.00003 WT carbonate-soluble Aβ40 0.07 ± 0.01 0.09 ± 0.01 NS WT carbonate-insoluble Aβ40 0.11 ± 0.02 0.13 ± 0.02 NS WT carbonate-soluble Aβ42 0.03 ± 0.003 0.04 ± 0.004 NS WT carbonate-insoluble Aβ42 0.12 ± 0.01 0.16 ± 0.01 NS Assay PDAPP plus saline PDAPP plus 10D5 p Plasma Aβ40a 60.5 ± 7 143.2 ± 17.6 0.0001 Plasma Aβ42a 21.9 ± 5.5 544 ± 253.9 0.04 Carbonate-soluble hippocampal Aβ40b 10.27 ± 7.22 8.88 ± 1.71 NS Carbonate-insoluble hippocampal Aβ40b 276.59 ± 122.23 190.13 ± 41.15 NS Carbonate-soluble hippocampal Aβ42b 37.82 ± 8.35 34.98 ± 5.38 NS Carbonate-insoluble hippocampal Aβ42b 1,275.15 ± 743.33 939.25 ± 232.44 NS
