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Neurobiology of Disease

State-Dependent Alterations in Hippocampal Oscillations in Serotonin 1A Receptor-Deficient Mice

Joshua A. Gordon, Clay O. Lacefield, Clifford G. Kentros and Rene Hen
Journal of Neuroscience 13 July 2005, 25 (28) 6509-6519; https://doi.org/10.1523/JNEUROSCI.1211-05.2005
Joshua A. Gordon
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Clay O. Lacefield
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Clifford G. Kentros
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Rene Hen
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Abstract

Mice lacking the serotonin 1A receptor (5-HT1AR) show increased levels of anxiety-related behavior across multiple tests and background strains. Tissue-specific rescue experiments, lesion studies, and neurophysiological findings all point toward the hippocampus as a potential mediator of the phenotype. Serotonin, acting through 5-HT1ARs, can suppress hippocampal theta-frequency oscillations, suggesting that theta oscillations might be increased in the knock-outs. To test this hypothesis, local field potential recordings were obtained from the hippocampus of awake, behaving knock-outs and wild-type littermates. The magnitude of theta oscillations was increased in the knock-outs, specifically in the anxiety-provoking elevated plus maze and not in a familiar environment or during rapid eye movement sleep. Theta power correlated with the fraction of time spent in the open arms, an anxiety-related behavioral variable. These results suggest a possible role for the hippocampus, and theta oscillations in particular, in the expression of anxiety in 5-HT1AR-deficient mice.

  • anxiety
  • theta rhythm
  • knock-out
  • 5-HT1A receptor
  • ripple oscillations
  • awake behaving recording

Introduction

Anxiety and its disorders are sensitive to serotonergic agents, including serotonin reuptake inhibitors and agonists of the serotonin 1A receptor (5-HT1AR) (Den Boer et al., 2000; Gordon and Hen, 2004). It is not surprising, therefore, that mice with a targeted deletion of the 5-HT1AR show increased levels of anxiety-related behaviors on several different tasks (Heisler et al., 1998; Parks et al., 1998; Ramboz et al., 1998). The recent finding of decreased 5-HT1AR binding in a positron emission tomography imaging study of panic disorder patients underscores the value of studying these mice as a model of human anxiety disorders (Neumeister et al., 2004). Understanding the precise mechanisms by which genetic deletion of the 5-HT1AR leads to increased anxiety-like behavior promises to shed light on the neurobiology of anxiety.

Using a tissue-specific, inducible rescue approach, Gross et al. (2002) showed that postsynaptic 5-HT1ARs are required during development for normal anxiety-like behavior in adulthood, suggesting that receptor deletion affects the formation of neural circuits that regulate anxiety. However, precisely which circuits are disrupted in the absence of the 5-HT1AR, and what specific functional properties are altered? Several lines of evidence suggest the hippocampus as a likely candidate region. Although the rescue directed expression of the 5-HT1AR to numerous areas, including the amygdala, the hippocampus and neocortex are the principle regions of overlap between wild-type and rescue expression patterns (Gross et al., 2002). The receptor is normally expressed most highly in the CA1 region of the hippocampus (Chalmers and Watson, 1991; Gross et al., 2002). Defects in hippocampal function have been documented in 5-HT1AR knock-outs, including impaired paired-pulse facilitation and inhibition (Sarnyai et al., 2000; Sibille et al., 2000). 5-HT1A receptor agonists injected directly into the hippocampus alter anxiety-related behaviors in the same tasks in which the knock-outs behave abnormally (Kostowski et al., 1989; Stefanski et al., 1993; File and Gonzalez, 1996; File et al., 1996). Finally, there are profound effects of direct 5-HT1AR activation on hippocampal physiology, including hyperpolarization of pyramidal neurons, as well as decreased magnitude of theta-frequency oscillations (Andrade and Nicoll, 1987; Hirose et al., 1990; Tada et al., 1999). Theta oscillations are 4-12 Hz waves present in local field potentials recorded throughout the hippocampus and related structures during navigation, among other behavioral states (Vanderwolf, 1969; Buzsaki, 2002).

The possibility that hippocampal circuitry, and theta oscillations in particular, might be abnormal in the 5-HT1AR knockouts is also suggested by the finding that anxiolytics, including benzodiazepines and 5-HT1AR agonists, decrease the propensity of the hippocampus to oscillate in the theta-frequency range (McNaughton et al., 1977; Zhu and McNaughton, 1994, 1995). These data suggest the hypothesis that theta-frequency oscillations might be increased in magnitude in 5-HT1AR knock-out mice. The present study tests this hypothesis by comparing local field potential recordings in awake, behaving knock-out and wild-type littermates. Findings reported here show that the knock-outs indeed have an increased magnitude of theta-frequency oscillations while they explore an elevated plus maze. Theta magnitude correlates with fraction open time, an anxiety-related behavioral variable (Pellow et al., 1985), and the increase in theta magnitude seen in the knock-outs persists across multiple exposures to the elevated plus maze, demonstrating that the difference is not attributable to novelty. These data raise the possibility that the hippocampus, and theta-frequency oscillations in particular, might play a role in the expression of anxiety.

Materials and Methods

Animals. 5-HT1AR knock-out mice were generated as described previously (Ramboz et al., 1998). All experiments were conducted blind to genotype on knock-out and wild-type littermates derived from hetero-zygote breeding pairs on a 129/Sv genetic background. The procedures described herein were conducted in accordance with National Institutes of Health regulations and approved by the Institutional Animal Care and Use Committees of Columbia University and the New York State Psychiatric Institute.

Surgical implantation. Custom microdrives were adapted from the method of Kentros et al. (2004) and were constructed using a Teflon platform suspended via stainless steel machine screws over Teflon nuts. The platform carried a 16-channel electrode interface board (Neuralynx, Tucson, AZ), and a 25 ga stainless steel cannula, through which were threaded two 75 μm Teflon-coated, blunt-cut tungsten wire electrodes. Two 30 ga solid-core wires were connected directly to the interface board to serve as reference and ground.

Surgery was conducted on animals aged 3-6 months. Animals were anesthetized with ketamine/xylazine (100 and 7 mg/kg, in saline) and placed in a stereotaxic frame. Body temperature was maintained by a temperature controller-regulated heating pad (Frederick Haer Company, Bowdoinham, ME). The skin overlying the dorsal surface of the skull was resected, and a 1.0 mm borehole was made stereotactically over the approximate position of the dorsal CA1 region of the hippocampus (coordinates: anteroposterior, -1.8 mm; lateromedial, 1.6 mm relative to bregma). Screw electrodes were implanted over the opposite frontal cortex (for reference) and cerebellum (for ground). The microdrive was then lowered into the borehole so that the tip rested 0.5 mm below the dural surface. The borehole was covered with warm petroleum jelly mixed with triple antibiotic ointment. The screw electrodes and microdrive were anchored to the skull with methyl methacrylate (Grip Cement; Dentsply, Milford, DE). Animals were then removed from the stereotaxic device and kept warm until recovered from anesthesia. Animals were allowed to recover for at least 1 week, until they returned to their preoperative weight (plus the weight of the installed microdrive, ∼2.5 g).

Electrophysiological recordings. Animals were attached to a unitary gain head-stage preamplifier (HS-16; Neuralynx) via a cable suspended on a pulley so as not to add any weight to the animal's head. Signals were amplified and bandpass filtered (1-9000 Hz) by a Lynx 8 programmable amplifier (Neuralynx) and acquired at 2000 Hz on a personal computer running Cheetah data acquisition software (Neuralynx). The animal's position was obtained by overhead video tracking of two light-emitting diodes affixed to the head stage. Position was measured at 30 Hz.

Locating the pyramidal layer. After recovery, animals were food deprived to ∼80% of bodyweight (preoperative plus microdrive weight). One to two times daily, animals were habituated to a familiar environment: a 35-cm-diameter circular arena constructed of wooden walls and a replaceable paper floor. Dim lighting (10 lux) was used as animals foraged for 20 mg pellets dropped from a dispenser above the arena every 60 s. Animals typically ate most of the pellets during this foraging period by the second exposure, and there was no difference by genotype in terms of the amount of pellets eaten. Animals received an additional ration of food sufficient to maintain their body weight on being returned to their home cages.

Between exposures to the familiar environment, local field potential recordings were obtained from both implanted electrodes while the animal rested in its home cage. Slow-wave (1-4 Hz) sleep periods were recognized by examining the broadband-filtered recordings on-line as by Buhl et al. (2003) and were easily distinguished from the periods of more regular, rapid theta activity that accompanied movement. The signal was simultaneously bandpass filtered at 100-350 Hz to search for ripple oscillations. The electrodes were advanced in 40 μm increments by turning each of the three screws supporting the microdrive one-eighth turn until ripples were first detected on either electrode. Subsequently, the microdrive was advanced in 20 μm (1/16 turn) increments until the magnitude of the ripples did not increase further. Once the pyramidal layer was located and the animals were exposed to at least five foraging sessions in the familiar environment, animals were returned to their home cages overnight before testing in the elevated plus maze.

Recording sessions. Recordings were obtained during a 10 min exposure to the elevated plus maze the next morning. The elevated plus maze was constructed of wood painted black and consisted of four arms, each 7.6 cm wide and 28 cm long, elevated 31 cm above the floor. Two of the arms were enclosed by 15-cm-high walls, whereas two were open except for a 1-cm-high lip at the edge. [This precise maze has been used in previous experiments to demonstrate increased anxiety in 5-HT1AR knock-outs (Gross et al., 2002).] Light levels were raised to 110 lux for the elevated plus maze exposures.

Animals were then removed from the elevated plus maze, allowed to rest in their home cages for 30 min, and then placed in the familiar environment to forage for 30 min under low-light conditions. Path length, speed, fraction time spent, and fraction path length in the center did not differ by genotype in the familiar environment under these conditions. Field potential recordings were obtained from the entire 30 min session; data from just the first 10 min are presented here for equivalence with the 10 min exposure to the elevated plus maze, although the results are indistinguishable from those obtained from the entire session.

After the familiar recording sessions, animals were placed in their home cages, fed, and rested for 30 min. The animals were then connected to the recording apparatus while still in the home cage, under low-light conditions (10 lux). Recordings were obtained while the animal rested motionless within the cage. Sleep periods were marked by an observer monitoring both the broadband (1-9000 Hz) and ripple-filtered (100-350 Hz) signals, as by Buhl et al. (2003). Slow-wave sleep periods were easily recognized, with 1-4 Hz activity predominating in the broadband signal and ripples clearly seen in the filtered signal. Periods of rapid eye movement (REM) sleep were also easily recognized, as the animal remained motionless, but theta activity (typically very regular at ∼6 Hz) dominated the broadband signal and ripples were absent. These markings were later used to segment the sleep sessions. Transitions between phases of sleep or between sleep and waking were discarded. Power spectra from REM periods yielded a sharp theta peak, whereas power spectra from slow-wave sleep periods yielded 1-4 and 150 Hz peaks but no peak in the theta range (see Fig. 8 and data not shown). The above procedures (elevated plus maze, familiar and sleep sessions) were repeated each morning for 3 d.

Depth series. For the depth series shown in Figure 1 A, a single wild-type animal was allowed to recover after surgery and then was food deprived to 80% of bodyweight. The animal was exposed five times to the familiar environment, and then a depth series was initiated. The electrodes were advanced three to four times daily in 40 μm (one-eighth turn) increments. Recordings were obtained during foraging in a familiar environment and during slow-wave sleep in the home cage at each increment.

Data analysis. Records were imported into Matlab (MathWorks, Natick, MA) for analysis. For theta analysis, the records were decimated to 400 Hz. Head speed and location were determined for each position record (30 Hz); speed was smoothed with a rectangular window of 10 samples (0.33 s). For overall power (see Figs. 1, 2, 6), a single power spectrum was calculated using the multi-taper method for the entire 10 min local field potential record. Mean power spectra were then calculated by genotype. For analysis of power by speed or location (see Figs. 3, 5), field potential records from each session were divided into short segments (at least 0.25 s and averaging 0.75-1 s per segment), during which the animal's speed or location fell within the assigned boundaries (for speed, 0-4, 4-8, 8-12, 12-16, 16-20, and >20 cm/s; for location, center, open arms, or closed arms). This procedure eliminated potential confounds, such as side-to-side head movements and deceleration, known to affect theta magnitude (Wyble et al., 2004). Distributions of segment lengths did not different by genotype. These segments were then concatenated, and power spectra were calculated for each speed range or location using the multi-taper method. Segments <0.25 s of constant speed or location were discarded. Theta power was calculated by summing the area under the curve of the power spectra from 4 to 12 Hz. Repeated-measures ANOVAs were performed using JMP (SAS Institute, Cary, NC) to test for differences in mean theta power across genotype, speed, and location.

Theta maps (see Fig. 4) were produced by first calculating theta power for a 0.5 s window around each position record. Theta power for a given position was then calculated by averaging the power for all visits to a given position. Maps did not differ when a smaller window was used to calculate theta (0.25 s). Speed maps were produced by averaging the speed (smoothed as described above) for all visits to a given position.

Ripples were analyzed by using the full records as acquired at 2000 Hz. Power spectra for Figure 8 A were computed on unfiltered segments of local field potential data taken during short-wave sleep epochs, typically tens of seconds in length, identified as described above. To measure ripple size, individual ripples were isolated from these segments by filtering the records at 100-350 Hz and identifying samples with values >7 SDs from the mean, as by Buzsaki et al. (2003). Mean peak-to-trough amplitude was determined for each animal by averaging the peak-to-trough amplitudes of each of the isolated ripples, and the grand means by genotype were compared (see Fig. 8). Power spectra for gamma were calculated from the decimated records (400 Hz sampling rate); the gamma/theta phase relationship was calculated using a Hilbert transform as by Buzsaki et al. (2003).

Factor analysis was conducted using the principle components function of JMP5.1 (SAS Institute). A varimax factor rotation was conducted on all factors with eigenvalues >1. All factor loadings >0.4 are displayed in Table 1. Power analyses on repeated-measures ANOVA were performed according to the methods of Murphy and Myors (1998).

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Table 1.

Factor analysis

Histology. After completion of recordings, electrolytic lesions were placed at the recording site by passing a small current through the recording electrode. Animals were then anesthetized with a lethal dose of ketamine and perfused with 10% Formalin. Sections, 40 μm thick, through the hippocampus were stained with cresyl fast violet and examined for the location of the lesions.

Results

The magnitude of theta oscillations varies several-fold throughout the depth of the hippocampus (Fig. 1A) (Buzsaki, 2002). To compare theta power across animals, therefore, a reliable indicator of depth within the hippocampus is crucial. The presence of high-frequency ripple oscillations was used to ensure that the tip of the recording electrode lay within the pyramidal layer, permitting accurate measurement of absolute theta power. Ripples are short, 25-75 ms waves with a peak frequency of ∼150 Hz in mice (Buzsaki et al., 2003), resulting from activation of CA1 pyramidal neurons by inputs from CA3 (Ylinen et al., 1995; Csicsvari et al., 1999).

5-HT1AR knock-out mice and littermate controls were implanted with tungsten microelectrodes in the cortex immediately above dorsal CA1, and animals were allowed to recover for a period of 7-10 d. The animals were then food deprived and exposed to a cylindrical, 35-cm-diameter “familiar environment” while tethered to the recording apparatus. The animals foraged for food pellets in the familiar environment for 30 min one to two times daily and a minimum of five times overall, to habituate to the environment, tethering, and handling. Between exposures to the familiar environment, mice were returned to their home cage, and local field potential recordings were made during slow-wave sleep. The electrode was advanced in 20-40 μm intervals, to a maximum of 320 μm/d, until ripples were clearly visible and did not increase further in magnitude. The experimental protocol began the day after the pyramidal layer was located or the day after the fifth exposure to the familiar environment, whichever came later. This procedure reliably located the pyramidal layer as revealed by electrolytic lesions placed at the conclusion of the recordings (Fig. 1B). All experiments were done blind to genotype.

  Figure 1.
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Figure 1.

Ripples mark the pyramidal layer. A, Depth profiles of ripple size (open squares) and theta power (filled triangles) through the CA1 region of the hippocampus. Depth is relative to initial electrode position. Arrows above indicated estimated location of boundaries between stratum oriens (s. oriens), pyramidale (s. pyr.), radiatum (s. rad.), and lacunosum moleculare (s.l.-m.). B, Nissl-stained section from the hippocampus of a mouse lesioned after completing the experiment. Arrow indicates location of lesion within the pyramidal layer.

5-HT1AR knock-outs have increased theta in the elevated plus maze

Once the pyramidal layer was located, field potential recordings were obtained while the animal explored an elevated plus maze, foraged in the familiar environment, and slept in its home cage. In both wild type and 5-HT1AR knock-outs, field potential recordings contained prominent oscillations; power spectra obtained from each condition revealed a distinct peak in the previously reported range of theta-frequency oscillations (Fig. 2) (Buzsaki et al., 2003). Comparing the power spectra across genotypes, it appeared that the theta peak was higher in knock-outs than in wild types in the elevated plus maze.

To quantify this difference, each recording was divided into segments in which the animal was moving at a given speed, because theta magnitude is known to increase with increasing speed (Vanderwolf, 1969). Spectra were then computed on these speed-divided records, and the area under the curve within the theta range (4-12 Hz) was summed. In recordings made while the animals explored the elevated plus maze, theta power in the knock-outs was consistently higher than in wild types, regardless of the speed at which the animals traveled (p < 0.03 by repeated-measures ANOVA) (Fig. 3A). The correlation of power with speed, although small, was present in both genotypes (p < 0.005 for the main effect of speed; p = 0.9 for speed × genotype interaction). The increase in theta power was likely attributable to increased amplitude of the oscillations as opposed to increased regularity because the width of the power spectra did not change (Fig. 2 and data not shown). Moreover, mean peak-to-trough amplitude of theta-filtered field potentials was similarly affected by genotype, although there were no differences in the interpeak interval distributions or autocorrelation functions (supplemental Fig. S1, available at www.jneurosci.org as supplemental material).

  Figure 2.
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Figure 2.

Top, Representative local field potential recordings from knock-out (KO) and wild-type (WT) mice navigating an elevated plus maze. Four seconds of data are shown; theta power is 0.081 and 0.051 for the knock-out and wild-type segments, respectively. Calibration: 0.5 mV, 0.5 s. Bottom, Averaged power spectra from all 5-HT1AR knock-out (solid lines) and wild-type (dashed lines) mice during exposure to an elevated plus maze. Inset, Expansion of power spectra in theta range. Thin lines are ±SEM. n = 14 animals per group.

  Figure 3.
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Figure 3.

Theta power is increased in 5-HT1AR knock-out mice during exposure to the elevated plus maze. Mean ± SEM of theta power (A, C, E) and peak frequency (B, D, F) during exposure to the elevated plus maze (A, B), a familiar environment (C, D), and during REM sleep in the home cage (E, F). For the elevated plus maze and familiar environment, power is calculated separately for epochs divided by the speed of the animal. Filled diamonds, 5-HT1AR knockouts; open squares, wild types. n = 10-14 animals per group.

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Figure 4.

Theta power is increased in 5-HT1AR knock-outs throughout the elevated plus maze. Maps of theta power (left) and speed (right) averaged by genotype. n = 14 animals per group. Open arms are arranged vertically; closed arms are arranged horizontally. KO, Knockouts; WT, wild types.

In the familiar environment and during REM sleep, the effect of genotype was minimal and not statistically significant (p = 0.56 and 0.52 by repeated-measures ANOVA and t test, respectively; genotype accounted for only 2.2% of the variance in the familiar environment compared with 24.9% in the elevated plus maze) (Fig. 3C,E). Indeed, when divided by speed, the tendency for knock-outs to have increased theta power in the familiar environment was so small that a sample size of 380 per group would be required to detect the difference with a power of 0.8. In all three conditions, theta frequency was not affected by genotype (Fig. 3B,D,F).

Theta power is increased throughout the elevated plus maze

To determine whether theta power is modulated by location with the maze, theta power was calculated for a 0.5 s window surrounding each position measurement. This continuous record of theta power was used to construct a map of theta power by position for each animal; the maps were then averaged across animals by genotype. In Figure 4, these averaged maps of theta power are shown along with similarly averaged maps of head speed. The overall difference in power between knock-outs and wild types is immediately apparent from the theta maps; throughout the maze, theta power was generally higher in the knock-outs than in the wild types (Fig. 4A,B). The lack of correlation between the power and speed maps, found in both genotypes, is also potentially interesting given the finding that, under some conditions, speed and theta have been related (Vanderwolf, 1969; Nitz and McNaughton, 1999). Power was mostly uniform throughout the maze, whereas speed was lowest in the center and higher on the open arms. The effect of genotype on theta power appears generally larger than the effect of speed.

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Figure 5.

5-HT1AR knock-outs have increased theta in the elevated plus maze regardless of location or speed. Theta power by location (A) or by speed within location (B-D). Filled bars, 5-HT1AR knock-outs (KO); open bars, wild types (WT). Other conventions as in Figure 3. n = 8-14 animals per group.

The spatial distribution of theta power was quantified by dividing the local field potential recordings by location and calculating theta power separately for the center, the open arms, and the closed arms. Regardless of location, power was greater in knock-outs than in wild-type animals (p < 0.05 by repeated-measures ANOVA) (Fig. 5A). To rule out the possibility that speed differences in each compartment accounted for the genotype differences, the records were then further subdivided by speed. The knock-out/wild-type differences in theta power were present regardless of speed in each location (Fig. 5B-D). Notably, theta power did not vary significantly by location within the elevated plus maze (compare individual speeds in Fig. 5B-D), nor was there evidence of a genotype by location interaction effect. There were also no statistically significant effects of genotype on speed.

Theta power correlates with anxiety-related behavior

The physiology cohort was too small to detect behavioral differences by genotype; to replicate the effect seen in the initial characterization of these knock-outs by Ramboz et al. (1998) with power of 0.80 requires 25-30 animals per group. Moreover, these animals were by necessity handled on a daily basis, a manipulation that decreases anxiety-like behavior in the elevated plus maze (Rodgers and Dalvi, 1997) and might make the behavioral differences between knock-outs and wild types more difficult to measure. Accordingly, no significant genotype differences were found in the two traditional measures of anxiety, fraction open arm entries (number of open entries/[number of open + number of closed entries]) or fraction time in the open arms (open time/[open + closed time]). Nonetheless, 5-HT1AR knock-outs did spend significantly less time in the center of the maze (mean ± SEM, 237 ± 29 and 354 ± 36 for knock-outs and wild types, respectively; p = 0.015 by t test). In a factor analysis, Ramboz et al. (1998) found center time to segregate with genotype and other anxiety-related variables.

To determine whether the differences in the knock-outs' behavior were attributable to anxiety-related factors, and what relationship these factors might have to theta power, a factor analysis was performed on the combined behavioral and physiological data. To prevent spurious correlations attributable to speed effects, theta power measurements from the middle speed range (8-12 cm/s) were used, although similar results were obtained with other speed ranges. Four factors were identified with eigenvalues >1, accounting for 82% of the total variance. Loadings >0.4 are shown for each of these factors in Table 1. Factor 1 is clearly an anxiety-related factor, reflecting correlations between fraction open entries and fraction open time. Factor 2 probably reflects exploratory activity, because the numbers of total and closed entries load onto this factor. Dips, an exploration and anxiety-associated variable, loads separately onto factor 4. Accounting for ∼16% of the total variance, factor 3 captures the correlation between fraction open time, center time, genotype, and theta power. This factor indicated that those animals with high theta power tended to spend less time in the open arms (relative to the closed arms), tended to spend less time in the center, and tended to be knock-outs.

Reflecting the strong influence of this factor, overall theta power in the elevated plus maze correlated with fraction open time (Fig. 6A). Those animals that spent less time in the open arms, indicative of greater anxiety, had higher theta power, regardless of genotype (p < 0.001; r2 = 0.25 by ANCOVA; p > 0.2 for an interaction with genotype). This relationship was not an artifact attributable to differences in speed, because correlations between fraction open time and theta power at any given speed were also significant (p = 0.02, 0.05, 0.03, 0.05, 0.03, and 0.02 for speeds 0-4, 4-8, 8-12, 12-16, 16-20, and >20 cm/s, respectively). The behavior, factor analysis, and correlation of theta power with fraction open time are all consistent with an association between the knock-out genotype, higher theta power, and higher levels of anxiety-related behavior.

Anxiety-related behavioral measures within the elevated plus maze do not habituate on repeated exposure, allowing the effects of novelty and anxiety to be differentiated (File et al., 1993; Rodgers and Shepherd, 1993; Fernandes and File, 1996; Holmes and Rodgers, 1998). If elevated theta power accompanies increased anxiety, the genotype difference in theta power should persist on multiple exposures to the elevated plus maze. To test this possibility, each animal was exposed to the elevated plus maze once daily for 3 d. Overall theta power was increased between groups across all 3 d of the task (p < 0.05 by repeated-measures ANOVA) (Fig. 6B) with no effect of day (p = 0.64). This result was again not attributable to differences in speed, because theta power was elevated regardless of speed on day 3 (p < 0.05 for the main effect of genotype; data not shown). The persistence of the elevation in theta magnitude argues that the increase is related to anxiety rather than novelty.

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Figure 6.

Theta power correlates with anxiety-like behavior. A, Linear regression (dashed line) of theta power on fraction open time in the elevated plus maze. B, Theta power in knockouts (KO) and wild types (WT) during three successive daily exposures to the elevated plusmaze. Conventions as in Figure 5. n = 8-14 animals per group.

Mice tend to avoid areas of the maze where theta power is highest

The data described above suggest a link between theta oscillations and the anxiety-like state induced by exposure to the elevated plus maze. Although stress hormone and ethological studies demonstrate increased anxiety-like behavior throughout the maze, animals appear to be more anxious in the open arms than in the closed arms (Pellow et al., 1985). One might therefore expect to find increased theta power in the open arms; such within-maze modulation was not seen in the averaged data (Figs. 4, 5). Variability in both behavior and theta power, however, may have obscured small intramaze differences in theta when averaged across animals. To explore this issue more precisely, correlations between intramaze differences in theta power and relevant behavior variables were examined. Because center time and fraction open time were shown to load with theta power and genotype by factor analysis, these variables were selected for additional study. Intramaze modulation of theta power was expressed as a ratio of power in the center or open arms to power in the closed arms; these ratios also have the virtue of controlling for interanimal variability in theta power. The time spent in the center of the maze correlated inversely with the ratio of power in the center to power in the closed arms (r2 = 0.26; p < 0.03 by ANCOVA; no interaction with genotype) (Fig. 7A). The correlation was only moderately robust to speed, however, with p values of 0.3, 0.003, 0.10, 0.4, 0.09, and 0.6 for 0-4, 4-8, 8-12, 12-16, 16-20, and >20 cm/s. A trend toward a similar correlation was seen between the ratio of power in the open arms to power in the closed arms and fraction open time (r2 = 0.12; p = 0.07 by ANCOVA; no interaction with genotype) (Fig. 7B). These correlations, although weak overall, suggest the possibility that higher theta power in a given compartment of the maze is associated with avoidance of that compartment.

  Figure 7.
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Figure 7.

Mice tend to avoid areas of the elevated plus maze where theta power is greatest. A, Linear regression (dashed line) of the ratio of theta power in the center to power in the closed arm, on time spent in the center of the maze. B, Linear regression (dashed line) of the ratio of theta power in the open arm to power in the closed arm, on fraction time spent in the open arm. KO, Knock-outs; WT, wild types.

Effects of the knock-out on fast hippocampal oscillations

The effect of 5-HT1AR deletion on high-frequency ripple oscillations during slow-wave sleep epochs was also examined. Power spectra computed from field potential recordings during these slow-wave sleep epochs revealed a peak at ∼150 Hz in both wild-type and knock-out mice (Fig. 8A), although the peak was markedly smaller in the knock-outs. A smaller peak in the power spectrum could be caused by less frequent or smaller ripples. To differentiate from these possibilities, 50-100 individual ripples were isolated from slow-wave sleep recordings from each animal, and mean peak-to-trough ripple amplitude was calculated (see Materials and Methods). Mean ripple amplitude was significantly higher for wild types than knock-outs (p < 0.01 by t test) (Fig. 8B). The frequency of identified ripple events did not differ by genotype (data not shown).

  Figure 8.
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Figure 8.

Ripples are decreased in size in 5-HT1AR knock-out mice. A, Power spectra of 5-HT1AR knock-out (KO) and wild-type (WT) mice during slow-wave sleep. Arrowhead indicates ripple peak. Arrows mark third, fourth, and fifth harmonic of 60 Hz noise. B, Mean peak-to-trough amplitude of isolated ripples from knock-outs and wild types. C, Theta power during REM sleep from the same recording sessions as in A and B. D, Plot of REM theta power versus ripple size. n = 10 animals per group.

Of course, smaller ripples and higher theta power could be the result of experimental error if the recording electrode was not precisely positioned in the pyramidal layer in the knock-outs. Theta power measured during REM sleep epochs taken from the same recording sessions, however, did not differ by genotype, making this explanation unlikely (Fig. 8C). Moreover, there was no correlation between REM theta power and ripple size in either genotype (Fig. 8D), as would be expected if electrode position accounted for the variation in our measurements. Despite the genotype difference in ripple size, no significant correlation between ripple size and anxiety-related behavioral measures or theta power in the elevated plus maze was seen (data not shown), suggesting that these effects are related only in that they result from genetic ablation of the 5-HT1AR.

As an additional control for proper electrode positioning, the relationship between gamma and theta activity during both elevated plus maze and familiar environment exposures was examined. Gamma oscillations are 40-100 Hz oscillations that are seen throughout the hippocampus, arising from synchronous activity in interneuron populations (Csicsvari et al., 2003; Mann and Paulsen, 2005). The magnitude of these oscillations did not differ between knock-outs and wild types in either environment (Fig. 9A,C). Gamma oscillations have a particular phase relationship with theta oscillations that depends on recording depth; gamma amplitude peaks just after the theta peak when the recording electrode is positioned within the pyramidal layer (Buzsaki et al., 2003). In our recordings, both genotypes had similar theta/gamma phase relationships (Fig. 9B,D). Peaks in gamma amplitude relative to theta phase were indistinguishable in the two genotypes (193.6 ± 2.3° and 195.7 ± 3.8° for knock-outs and wild types, respectively, mean ± SEM; n = 14 per genotype).

Discussion

Mice lacking the 5-HT1AR have a robust phenotype of increased anxiety (Heisler et al., 1998; Parks et al., 1998; Ramboz et al., 1998). To identify the consequences of the gene deletion responsible for this phenotype, hippocampal activity was recorded from awake, behaving 5-HT1AR knockout mice. These mice exhibited a state-dependent increase in the magnitude of hippocampal theta-frequency oscillations. These oscillations were increased in an anxiety-provoking environment, the elevated plus maze, but not in a familiar environment or during REM sleep. Theta power was higher in those animals that showed higher levels of anxiety-related behavior, and the increase in theta power in the knock-outs persisted across multiple exposures to the elevated plus maze. Together, these results support the hypothesis that abnormal hippocampal activity may contribute to the anxiety-like phenotype seen in the knock-outs, although alternative explanations must be considered.

Theta oscillations and anxiety

The idea that hippocampal theta oscillations play a role in anxiety is not new. Indeed, Gray and McNaughton (2000) have championed the hypothesis for the past two decades. The idea originated with the findings that lesions of the septohippocampal circuit responsible for driving theta oscillations disrupt anxiety-like behavior on such tasks as the open field, elevated plus maze, social interaction, light-dark, shock-probe burying, and hyponeophagia tests (Miller et al., 1986; Deacon et al., 2002; Degroot and Treit, 2003; Bannerman et al., 2004). Recent studies have generally shown that the anxiety phenotype arises specifically from ventral hippocampal lesions (Kjelstrup et al., 2002; Bannerman et al., 2003; McHugh et al., 2004). For technical reasons (it is difficult to reliably establish precise electrode localization in ventral hippocampal subregions in vivo), the current work was performed in dorsal hippocampus. The implications of the present findings for ventral hippocampal activity in the knock-outs are unknown, although theta oscillations remain highly coherent with distance, even across hemispheres (Bullock et al., 1990; Buzsaki, 2002; Buzsaki et al., 2003).

The behavioral differences seen between the knock-outs and wild types in the elevated plus maze in this study were subtle and did not include genotype differences in pharmacologically validated measures of anxiety-related behaviors (Pellow et al., 1985). This raises the possibility that the theta phenotype is related to other behavioral factors. For instance, 5-HT1AR knock-outs might have difficulty with the cognitive demands placed on them by environments such as the plus maze; deficits in spatial learning have been described in these mice (Sarnyai et al., 2000; Wolff et al., 2004). An intriguing possibility is that the theta oscillation abnormality contributes to both anxiety and spatial learning phenotypes, by altering a fundamental functional property of the hippocampus (Gray and McNaughton, 2000). Measuring theta oscillations during additional behavioral tasks would help differentiate from among these and other possibilities.

  Figure 9.
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Figure 9.

Gamma oscillations are not different by genotype. A, C, Power spectra during exposure to the elevated plus maze (A) and the familiar environment (C) expanded to highlight the gamma-frequency peak. B, D, Relationship between gamma oscillation amplitude and theta phase in the elevated plus maze (B) and the familiar environment (D). n = 10-14 animals per group. Conventions as in Figure 2. The 60 Hz artifact was removed in graphic. KO, Knock-outs; WT, wild types.

How does 5-HT1AR deficiency lead to altered hippocampal oscillations?

From the limited literature on serotonergic effects on ripple oscillations, it seems likely that a decrease in ripple amplitude cannot be explained by a simple lack of the receptor in the adult. It is difficult to reconcile decreased ripple size, which suggests less excitation of CA1 pyramidal neurons (Csicsvari et al., 1999), with the absence of an inhibitory receptor. A recent report showed that pharmacological blockade of the 5-HT1AR with the specific antagonist WAY 100635 (N-[2-[4-(2-methoxyphenyl)-1-piperazinyl] ethyl]-N-(2-pyridinyl)cyclo-hexane carboxamide trihydrochloride) resulted in less frequent but normal-sized ripples (Ponomarenko et al., 2003) as opposed to the smaller but equally frequent ripples seen here in the knock-outs. These data argue that the change in ripple size seen in the knock-outs is likely attributable to an indirect effect of 5-HT1AR deletion, perhaps as a consequence of altered hippocampal development. Alternatively, serotonergic drugs are known to affect the sleep cycle, and 5-HT1AR knock-out mice have altered sleep patterns (Boutrel et al., 2002; Monaca et al., 2003), suggesting that the less effective synchronization evident in smaller ripples could be a consequence of sleep disruption.

The story is also complex with regard to the better-studied theta oscillations. Serotonin is capable of modulating theta through several different receptors and pathways (Staubli and Xu, 1995; Vertes and Kocsis, 1997; Kitchigina et al., 1999; Vertes et al., 2004). Manipulating serotonin levels with depleting agents or precursors suggests that serotonin may enhance theta, perhaps specifically atropine-resistant “type 1” theta associated with exploratory behavior (Vanderwolf and Baker, 1986; Robertson et al., 1991). (The theta oscillations measured here are likely type 1, although no pharmacological dissection was performed.) However, lesioning or silencing the serotonergic raphe nuclei enhances theta, suggesting that serotonin normally suppresses theta oscillations (Maru et al., 1979; Vertes et al., 1994; Marrosu et al., 1996; Nitz and McNaughton, 1999). The suppressive effects of serotonin on theta are likely to be mediated, at least in part, through hippocampal 5-HT1ARs, because direct hippocampal injection of 5-HT1AR agonists decreases theta power (Hirose et al., 1990). Such experiments suggest that the lack of the 5-HT1AR in the adult knock-out might prevent the normal suppression of theta power by serotonin.

This suggestion is not straightforwardly compatible with the state dependence of the theta increase seen in the knock-outs. To explain the finding that theta is only increased in the elevated plus maze, one would have to assert that serotonergic neurons are significantly active only in this environment. A state-specific increase in serotonin activity, acting on a hippocampus lacking the inhibitory 5-HT1AR, would then fail to suppress theta in the knock-outs. The evidence for serotonergic activation during exposure to stressful or anxiety-provoking situations is mixed (Silveira et al., 1993; Jacobs and Fornal, 1997; Voigt et al., 1999; Rex et al., 2003). Microdialysis experiments in 5-HT1AR knock-outs are also inconclusive; exposure to an anxiety-provoking novel open field increases frontal cortical but not hippocampal serotonin release in knock-outs and wild types (Parsons et al., 2001).

An alternative hypothesis for the state-dependent increase in theta magnitude is that 5-HT1AR deficiency during development results in a fundamental alteration in the circuitry of the hippocampus; this hypothetical circuit alteration would affect regulation by serotonin or other processes active in anxiety-provoking situations (Gross et al., 2002). A number of studies implicate serotonin in the development of the hippocampus, including regulation of neurite outgrowth and synaptogenesis (Azmitia and Whitaker-Azmitia, 1997; Sodhi and Sanders-Bush, 2004). We have shown recently an increase in dendritic branching, dendritic length, and total number of spines in the proximal apical dendrites of CA1 pyramidal neurons in 5-HT1AR knock-outs (J. E. Monckton, K. Klemenhagen, J. A. Gordon, C. Gross, R. Meylan, E. Prolong, R. Hen, and J.-P. Hornung, unpublished observation). These morphological changes are accompanied by an apparent increase in synaptic efficacy of the CA3 inputs onto these dendrites. Synaptic currents induced by these very inputs form part of the driving force of theta oscillations (Buzsaki, 2002). Although the CA3 inputs comprise a source that can act independently from the sources measured in the pyramidal layer (Kocsis et al., 1999), it is conceivable that the morphological and physiological changes seen at the level of the CA1 pyramidal neurons are related to the changes in hippocampal oscillations observed in vivo.

Functional significance of increased theta oscillations

Several roles for theta oscillations in hippocampal function have been proposed, most pertaining to models of synaptic plasticity-based learning (Huerta and Lisman, 1995; Hyman et al., 2003). Variation in the amplitude of theta oscillations might therefore alter plasticity. Some have argued that temporal encoding using the variable timing of firing of a hippocampal cell with relation to the theta cycle, known as phase precession, could mediate sequence learning and novelty detection (Mehta et al., 1997; Lisman and Otmakhova, 2001). How these concepts might relate to anxiety-like behavior is unclear, except that most novel environments provoke anxiety.

A more straightforward link between theta and anxiety is suggested by a recent study of fear conditioning. Seidenbecher et al. (2003) examined local field potentials in the hippocampus and amygdala during the presentation of a fear-conditioned stimulus. During normal exploration and during presentation of an unconditioned tone, there was no evidence of theta-frequency oscillations in the amygdala, despite strong theta in the hippocampus. On presentation of a fear-conditioned tone, theta oscillations emerged in the amygdala, and these oscillations were tightly correlated with hippocampal theta. These findings suggest that theta oscillations can be propagated to downstream targets, likely attributable to the coordinated activity of output neurons in CA1 and the subiculum timed to the theta cycle. Thus, the increased theta magnitude seen here in 5-HT1AR knock-outs could lead to increased activity in downstream targets.

Conclusion

The findings described here demonstrate significant abnormalities in hippocampal activity in awake, behaving 5-HT1AR knockout mice. The magnitude of theta-frequency oscillations is increased in an anxiety-provoking environment and correlates with an anxiety-related behavioral variable. This increase appears to be related to anxiety, as opposed to novelty, because it persists on multiple exposures to the elevated plus maze. These findings suggest a role for hippocampal theta oscillations in the expression of anxiety-related behaviors. The results form a framework for additional study into the consequences of 5-HT1AR deletion on the neurophysiology of the neural circuits underlying anxiety.

Applying the current findings to the study of humans with anxiety disorders is theoretically straightforward. Theta oscillations are seen in human electroencephalograms, and, although the precise anatomical origins of these oscillations are still debated, they are thought to arise from corticolimbic interactions, perhaps driven by hippocampal theta (Basar et al., 2001; Kahana et al., 2001; Cantero et al., 2003). Conflicting reports differ on the relationship between theta in human electroencephalograms and anxiety traits or disorders (Mizuki et al., 1992, 1994; Saletu-Zyhlarz et al., 1997; Romano-Torres et al., 2002; Aftanas et al., 2003; Sachs et al., 2004). A drawback to many of these studies is that EEG recordings were obtained at rest or during cognitive tasks; the results reported here would suggest that symptom provocation studies might be an effective way to look for disorder-related increases in theta power in humans with anxiety disorders.

Footnotes

  • This work was supported by National Institutes of Health Grants P01 MH48125 (R.H.) and K08 MH 069823 (J.A.G.). R.H. is a National Alliance for Research on Schizophrenia and Depression (NARSAD) Distinguished Investigator. J.A.G. is a NARSAD-Bowman Family Foundation Young Investigator and was supported by a Pfizer Biological Psychiatry Postdoctoral Fellowship and a Sackler Foundation Fellowship in Developmental Psychobiology. We thank E. Kandel, N. Agnihotri, G. Buzsaki, D. Buhl, and K. Harris, as well as other members of the Buzsaki laboratory, for providing advice, assistance, and software routines for performing the experiments and analyses described here. J. Mori assisted with the ripple analysis. We also thank D. Leonardo, A. Dranovsky, G. Buzsaki, and C. D. Salzman for helpful comments on this manuscript.

  • Correspondence should be addressed to Joshua A. Gordon, Center for Neurobiology and Behavior, Department of Psychiatry, Columbia University/New York State Psychiatric Institute, 1051 Riverside Drive, Unit 87, New York, NY 10032. E-mail: jg343{at}columbia.edu.

  • Copyright © 2005 Society for Neuroscience 0270-6474/05/256509-11$15.00/0

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The Journal of Neuroscience: 25 (28)
Journal of Neuroscience
Vol. 25, Issue 28
13 Jul 2005
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State-Dependent Alterations in Hippocampal Oscillations in Serotonin 1A Receptor-Deficient Mice
Joshua A. Gordon, Clay O. Lacefield, Clifford G. Kentros, Rene Hen
Journal of Neuroscience 13 July 2005, 25 (28) 6509-6519; DOI: 10.1523/JNEUROSCI.1211-05.2005

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State-Dependent Alterations in Hippocampal Oscillations in Serotonin 1A Receptor-Deficient Mice
Joshua A. Gordon, Clay O. Lacefield, Clifford G. Kentros, Rene Hen
Journal of Neuroscience 13 July 2005, 25 (28) 6509-6519; DOI: 10.1523/JNEUROSCI.1211-05.2005
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