Figure 5.
AAV-Homer2b reverses the genotypic differences in EtOH behavior. a-a″, Representative micrographs of immunostaining for AAV-transfected HA-tagged Homer2b in accumbens. Arrowheads indicate glial scar from injection (a) and fiber (black fill) or soma (white fill) labeling (a″). b, AAV-Homer2b infusion shifted EtOH (EtOH) preference to the left in both WT and KO mice (genotype × dose × AAV interaction, F(3,96) = 4.38; p = 0.006). c, AAV-Homer2b infusion reduced the preference for water in KO mice (genotype × AAV, F(1,35) = 9.95; p = 0.003). d, AAV-Homer2b infusion restored EtOH intake (on a gram per kilogram body weight basis) in KO mice to the level exhibited by WT animals (genotype × AAV, F(1,35) = 7.38; p = 0.011). e, Whereas planned comparisons revealed genotypic differences in the capacity of eight pairings of 3 g/kg EtOH to elicit place conditioning were observed in between AAV-GFP WT and KO mice (F(1,16) = 5.20; p = 0.04), this difference was not apparent between AAV-Homer2b WT and KO animals (F(1,16) = 1.75; p = 0.21). f, Planned comparisons also revealed that AAV-Homer2b reversed the genotypic difference in locomotor adaptation produced by eight injections of 3 g/kg EtOH (for GFP, F(1,16) = 10.41, p = 0.006; for Homer2b, F(1,16) = 0.57, p = 0.46). Data in b-f represent the mean ± SEM of the number of animals indicated. +p < 0.05 versus respective WT; #p < 0.05 versus respective AAV-GFP.
