Figure 4.
Modulation of glycine-activated currents by WIN 55,212-2. a, Left, Traces of IGly induced by 100 μm Gly obtained in control, in the presence of WIN 55,212-2 (1 μm), and after washout of drug (gray line) (Vh = -100 mV). Middle and right, The same traces are superimposed and normalized. b, Statistics of the effects of WIN 55,212-2 on the amplitude, τdes, and ton (n=11) of IGly. c-e, Concentration dependence of the WIN 55,212-2 effects on IGly kinetics. c, IGly traces recorded at various concentration of WIN 55,212-2 ranged from 100 nm to 2 μm measured in the same cell are normalized and superimposed. d, e, Summary plots showing the effect of increasing the WIN 55,212-2 concentrations on τdes (d) and ton (e) measured at Vh = -100 mV (n = 4). Data are plotted as a fraction of the maximal glycine current in control. f-h, Voltage dependence of the IGly modulation by the WIN 55,212-2. f, Left, IGly representative traces obtained in control condition and in the presence of 1 μm WIN 55,212-2 at holding potentials of -100 mV and +20 mV. Middle and right, The same traces are superimposed and normalized. Statistics for the WIN 55,212-2 effects on τdes (g) and ton (h) at holding voltages of -100 and +20 mV.