In the article “Chronic Enhancement of the Intrinsic Growth Capacity of Sensory Neurons Combined with the Degradation of Inhibitory Proteoglycans Allows Functional Regeneration of Sensory Axons through the Dorsal Root Entry Zone in the Mammalian Spinal Cord,” by Michael P. Steinmetz, Kevin P. Horn, Veronica J. Tom, Jared H. Miller, Sarah A. Busch, Dileep Nair, Daniel J. Silver, and Jerry Silver, which appeared on pages 8066–8076 of the August 31, 2005 issue, the third contributor to the unpublished observations mentioned in the sixth paragraph of the Discussion is A. I. Basbaum. In addition, Figures 1 and 2 should have printed in color rather than black and white. The correct color versions of both figures, as well as each corresponding legend, are printed here.
Combination of zymosan and ChABC enhances axon crossing of the inhibitory rim. A, Quantification of axon crossing of the inhibitory rim was quantified by mean pixel density. The most robust regeneration was seen with the combinatorial treatment. Statistical differences between each group and control were as follows: 7 d zymosan, p < 0.0001; ChABC, p < 0.001; zymosan plus ChABC, p < 0.001. B, Representative image of axons (green) unable to traverse the inhibitory rim (red representing CSPGs). C, Representative image of slight but significant crossing of the inhibitory rim (red) by axons (green, arrow) pretreated with zymosan. Similar results were observed with ChABC treatment. D, Zymosan pretreatment coupled with ChABC CSPG degradation (red representing digested CSPG) produced robust axon (green β-tubulin III) crossing of the inhibitory rim (arrow). E, Seven day zymosan pretreatment with ChABC application can result in obliteration of the spot rim (arrow) by β-tubulin III-positive adult DRG processes (green). Scale bar: B–E, 50 μm.
Combination treatment increases the number of satellite glia. A, Quantification of S-100 staining using pixel density. Significantly greater numbers of satellite glia were present after the combinatorial treatment (p < 0.001). B, Image demonstrating satellite glia (red) in the vicinity of the rim and the fact that, although there were more glia in general, DRG sensory axons (green) were capable of crossing the inhibitory rim in the absence of close contact with the satellite cells. Scale bar, 50 μm.
