The Nonkinase Phorbol Ester Receptor α1-Chimerin Binds the NMDA Receptor NR2A Subunit and Regulates Dendritic Spine Density

We thank Drs. Louis Lim and Christine Hall (Institute of Neurology, London, UK) for providing α2-chimerin in pXJ40-HA and pXJ40-GFP and anti-α2-chimerin antibody. We also thank Dr. Marcelo Kazanietz (The Center for Experimental Therapeutics and Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA) for providing α1-chimerin in pLexA vector. Plasmids expressing wild-type and mutant Rac1 isoforms were a generous gift from Dr. Ann Marie Pendergast (Duke University), and a plasmid expressing NR2A was a generous gift from Dr. Robert Wenthold (Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD). We thank Dr. Fernando Ribeiro-Neto (University of Pittsburgh, Pittsburgh, PA) for critical discussions.

Correspondence should be addressed to Antonius VanDongen, Department of Pharmacology and Cancer Biology, Duke University Medical Center, P.O. Box 3813, Durham, NC 27710. E-mail: vando005{at}mc.duke.edu.

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