Figure 1.
Diagram of primary tissue dissected for in vitro assay of stem-cell activity. A2-mm-thick coronal slice was cut between -1.22 and -2.70 mm, relative to bregma, as determined from an adult mouse brain atlas (Paxinos and Franklin, 2001). The slice was placed caudal side up and the tissue dissected using two pairs of curved forceps. First, the midbrain was removed, and then the hippocampus, as a whole, was separated gently from the corpus callosum. Finally, the pLV, including the corpus callosum, was pinched away from the cortex. Hippo, Hippocampus; LV, lateral ventricle. This image is from Paxinos and Franklin (2001) with permission.