Toll-Like Receptor 4 on Nonhematopoietic Cells Sustains CNS Inflammation during Endotoxemia, Independent of Systemic Cytokines

Effect of LPS repurification on gene transcription in TLR4 mutant mice. Film autoradiographs show induction of IκBα (a-f) in brainstem sections of mice 2 h after LPS administration. C3H/HeOuJ (a-c) or C3H/HeJ mice (d-f) were injected intraperitoneally with saline (a, d); LPS serotype 055:B5 (b, e) or repurified LPS (c, f). bv, blood vessel.

Hematopoietic reconstitution replaces >95% of resident CD45⁺ cells. We assessed the efficiency of hematopoietic chimerism in our experimental protocol by making MHC class I mismatched chimeras. Bone marrow from B6 mice (MHC haplotype b) or HeOuJ mice (MHC haplotype k) were transferred into irradiated HeJ mice (MHC haplotype k). Two months after reconstitution, spleens were isolated from chimeric mice. Single-cell suspensions from spleen were labeled with antibodies to CD45 as well as antibodies specific to the MHC Class I molecules (H-2K) of the k (H-2K^k) or b (H-2K^b) haplotype. Staining was analyzed on a BD FACScalibur. Plots show MHC staining observed on cells that are CD45⁺ (gated). In the case of mice receiving HeOuJ bone marrow (a), ∼96% of the CD45⁺ cells were of the HeOuJ origin. In the case of mice receiving the B6 bone marrow (b), ∼91% of the cells stained for the B6 Class I molecule. Although ∼2% of the cells fell within the bottom right quadrant, suggesting a staining by the k haplotype, there was no distinct population corresponding to the MHC-expressing cells (compare bottom right quadrants in a). This allows us to conclude that our protocols were successful in generating at least 90-95% donor-derived hematopoietic chimerism with no significant contribution from the recipient toward this compartment. Although this analysis was done using MHC-mismatched chimeras involving a protocol for depletion of resident NK cells before reconstitution, those WT and HeJ mice that were used to construct the experimental chimeras did not involve a mismatch at the MHC locus.

Induction of peripheral cytokines in TLR4 chimeric mice. Mice were challenged with repurified LPS (1 mg/kg, i.p.), and cytokines in serum samples collected 2 or 6 h later were detected using ELISA (a-d). TNFα (a), IL-1β (b), IL-12 p70 (c), and IFNγ (d) levels (in picograms per milliliter) were determined for each chimeric class (details in the key). Error bars represent means ± SEMs from three or four mice per group. Appropriate post hoc tests were used to compare group means against the control WT → WT chimeric mice after one-way ANOVA. ^*p < 0.05; ^**p < 0.001. Autoradiographic images show TNFα mRNA in the spleen of chimeric mice: HeJ → HeJ (e), WT → WT (f), WT → HeJ (g), and HeJ → WT (h) 2 h after LPS administration.

Early inflammatory response to LPS in CNS of chimeric mice. Film autoradiographs (a-h) show mRNA induction in brains of mice killed 2 h after intraperitoneal LPS. TNFα (a-d) and IκBα (e-h) mRNA in brainstem sections of the chimeric mice: HeJ → HeJ (a, e), WT → WT (b, f), WT → HeJ (c, g), and HeJ → WT (d, h). Induction of TNFα (i) and IκBα (j) mRNA in chimeric groups (details in key) were quantified by densitometry of film images. Data representing mean integrated density ± SEM for each mRNA were obtained from three or four mice per group. One-way ANOVA was followed by appropriate post hoc analysis to compare chimeric group means against the control WT → WT group. ^**p < 0.001.

Late inflammatory response to LPS in CNS of chimeric mice. Representative film autoradiographs (a-p) show proinflammatory mRNA induction in brains of mice killed 6 h after intraperitoneal LPS. IκBα, IL-1β, TLR2 (i-l), and CD14 (m-p) mRNAs were detected by radioactive in situ hybridization on brainstem sections from chimeric mice: HeJ → HeJ (a, e, i, m), WT → WT (b, f, j, n), WT → HeJ (c, g, k, o), and HeJ → WT (d, h, l, p). Quantified expression levels are shown (q-t). IκBα (q), IL-1β (r), TLR2 (s), and CD14 (t) mRNA levels are plotted as mean integrated density ± SEM from three or four mice per chimeric class. Individual group means were compared against the control WT → WT group, and statistical significance was established by one-way ANOVA and appropriate post hoc tests. ^*p < 0.05; ^**p < 0.001.