Figure 6.
Cannabinoids prevent βA-induced microglial activation in vitro. a, Immunostaining of cultured microglia with anti-OX42 (top), CB1 (middle), and CB2 (bottom) antibodies. b, Fibrillar βA1-40 (fib), but not soluble βA1-40 (sol), induced a rod-like morphology, which was prevented by HU-210 (HU). c, Cannabinoids [HU-210 (HU), WIN55,212-2 (WIN), and JWH-133 (JWH), at 100 nm for 4 h] prevented TNF-α release and mitochondrial activity, as induced by fibrillar βA1-40 (500 nm). TNF-α release in controls was 26.1 ± 4.5 pg/ml. Results are mean ± SEM of n = 4-6; *p < 0.05 and **p < 0.01 compared with soluble βA1-40-treated control cultures; #p < 0.05, ##p < 0.01 compared with fibrillar βA1-40-treated cultures (ANOVA with Bonferroni's post hoc test).