Figure 3.
Induction of LTD at various longitudinal muscle fibers M4, M6, and M12 (A, B) and at different axons (C–E). A, Representative traces. Calibration: 20 nA, 2 min. B, Summary of normalized EJC amplitude. n = 4, 6, 4 for M4, M6, M12, respectively. C, A representative recording on M6 by retrograde stimulation of axon 2 on the nerve branch to M12. Calibration: 10 nA, 2 min. D, Summary of experiments on stimulating axon 2. n = 4. E, Comparison of the responses on M6 evoked by stimulating axon 2 and the total EJC evoked by stimulating the segmental nerve. Note that the total EJC amplitudes of the segmental nerve were larger than those of axon 2 after induction of LTD, suggesting that both axons were responsive after LTD induction. n = 4 for either group.