Requirement of Akt to Mediate Long-Term Synaptic Depression in Drosophila

The akt mutant (akt⁴²²⁶) exhibited similarly impaired LTD at lower Ca²⁺ concentration (0.2 mm Ca²⁺, at which LTD was the most pronounced in wild-type larvae) or by increased stimulation frequency (40 Hz). A, B, Reduction of LTD in the akt mutant at 0.2 mm Ca²⁺ was similar to LTD at 0.4 mm Ca²⁺ (refer to Fig. 1E). A, Representative recordings for wild type (WT) and akt⁴²²⁶. Calibration: 20 nA, 2 min. B, Summary of four recordings. C, D, LTD induced by 40 Hz tetanus was also primarily impaired in the akt mutant. C, Representative traces. Calibration: 20 nA, 2 min. D, Summary of four recordings.