Characterization of a Soluble Ligand Binding Domain of the NMDA Receptor Regulatory Subunit NR3A

This work was supported by the intramural research program of the National Institute of Child Health and Human Development, National Institutes of Health (NIH), Department of Health and Human Services. We thank Drs. Stuart Lipton and Dongxian Zhang for the gift of plasmids encoding the cDNAs for NR3A and NR3B, and Dr. H. Furukawa for sharing the purification protocol for NR1S2S2. Mass spectral analysis and Edman sequencing was performed by Howard Jaffe [Protein/Peptide Sequencing Facility, National Institute of Neurological Disorders and Stroke (NINDS), NIH]. Nucleic acid sequencing was performed by the NINDS DNA sequencing facility.