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Microtargeted Gene Silencing and Ectopic Expression in Live Embryos Using Biolistic Delivery with a Pneumatic Capillary Gun

Orit Shefi, Claire Simonnet, Michael W. Baker, James R. Glass, Eduardo R. Macagno and Alex Groisman
Journal of Neuroscience 7 June 2006, 26 (23) 6119-6123; DOI: https://doi.org/10.1523/JNEUROSCI.1237-06.2006
Orit Shefi
1Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0376, 2Department of Physics, University of California, San Diego, La Jolla, California 92093-0374, and 3Seashell Technology, La Jolla, California 92037
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Claire Simonnet
1Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0376, 2Department of Physics, University of California, San Diego, La Jolla, California 92093-0374, and 3Seashell Technology, La Jolla, California 92037
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Michael W. Baker
1Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0376, 2Department of Physics, University of California, San Diego, La Jolla, California 92093-0374, and 3Seashell Technology, La Jolla, California 92037
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James R. Glass
1Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0376, 2Department of Physics, University of California, San Diego, La Jolla, California 92093-0374, and 3Seashell Technology, La Jolla, California 92037
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Eduardo R. Macagno
1Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0376, 2Department of Physics, University of California, San Diego, La Jolla, California 92093-0374, and 3Seashell Technology, La Jolla, California 92037
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Alex Groisman
1Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0376, 2Department of Physics, University of California, San Diego, La Jolla, California 92093-0374, and 3Seashell Technology, La Jolla, California 92037
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.1237-06.2006
PubMed 
16763019
Published By 
Society for Neuroscience
History 
  • Received March 22, 2006
  • Revision received April 21, 2006
  • Accepted April 21, 2006
  • First published June 7, 2006.
  • Version of record published June 7, 2006.
Copyright & Usage 
Copyright © 2006 Society for Neuroscience 0270-6474/06/266119-05$15.00/0

Author Information

Author contributions

Disclosures

    • Received March 22, 2006.
    • Revision received April 21, 2006.
    • Accepted April 21, 2006.
  • This work was supported by National Institutes of Health Grants 1 R41 MH076332-01 (C.S., J.R.G., and A.G.) and 1R01 NS043546 (O.S., M.W.B., and E.R.M.). We thank Sandra Peterson for technical assistance.

  • Correspondence should be addressed to either Orit Shefi or Alex Groisman, Division of Biological Sciences, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0376. Email: oshefi{at}ucsd.edu or agroisman{at}ucsd.edu

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The Journal of Neuroscience: 26 (23)
Journal of Neuroscience
Vol. 26, Issue 23
7 Jun 2006
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Microtargeted Gene Silencing and Ectopic Expression in Live Embryos Using Biolistic Delivery with a Pneumatic Capillary Gun
Orit Shefi, Claire Simonnet, Michael W. Baker, James R. Glass, Eduardo R. Macagno, Alex Groisman
Journal of Neuroscience 7 June 2006, 26 (23) 6119-6123; DOI: 10.1523/JNEUROSCI.1237-06.2006

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Microtargeted Gene Silencing and Ectopic Expression in Live Embryos Using Biolistic Delivery with a Pneumatic Capillary Gun
Orit Shefi, Claire Simonnet, Michael W. Baker, James R. Glass, Eduardo R. Macagno, Alex Groisman
Journal of Neuroscience 7 June 2006, 26 (23) 6119-6123; DOI: 10.1523/JNEUROSCI.1237-06.2006
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Keywords

  • RNAi
  • ectopic expression
  • biolistic delivery
  • axon growth
  • Hirudo medicinalis
  • netrin
  • gene gun

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