Abstract
The neurohypophysial nonapeptides vasopressin (VP) and oxytocin (OT) modulate a broad range of cognitive and social activities. Notably, in amphibians, vasotocin (VT), the ortholog of mammalian VP, plays a crucial role in the control of sexual behaviors. Because several neurosteroids also regulate reproduction-related behaviors, we investigated the possible effect of VT and the OT ortholog mesotocin (MT) in the control of neurosteroid production. Double immunohistochemical labeling of frog brain sections revealed the presence of VT/MT-positive fibers in close proximity of neurons expressing the steroidogenic enzymes 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (3β-HSD) and cytochrome P450 17α-hydroxylase/c17, 20-lyase (P450C17). High concentrations of VT and MT receptor mRNAs were observed in diencephalic nuclei containing the 3β-HSD and P450C17 neuronal populations. Exposure of frog hypothalamic explants to graded concentrations of VT or MT produced a dose-dependent increase in the formation of progesterone, 17-hydroxypregnenolone, 17-hydroxyprogesterone, and dehydroepiandrosterone. The stimulatory effect of VT and MT on neurosteroid biosynthesis was mimicked by VP and OT, as well as by a selective V1b receptor agonist, whereas V2 and OT receptor agonists had no effect. VT-induced neurosteroid production was completely suppressed by selective V1a receptor antagonists and was not affected by V2 and OT receptor antagonists. Concurrently, the effect of MT on neurosteroidogenesis was markedly attenuated by selective OT and V1a receptor antagonists but not by a V2 antagonist. The present study provides the first evidence for a regulatory effect of VT and MT on neurosteroid biosynthesis. These data suggest that neurosteroids may mediate some of the behavioral actions of VT and MT.
- neurosteroid biosynthesis
- vasotocin
- mesotocin
- 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase
- cytochrome P450 17α-hydroxylase/c17, 20-lyase
- hypothalamus
Introduction
Brain neurons and/or glial cells have the capability of synthesizing bioactive steroids from cholesterol in very much the same as peripheral steroidogenic tissues (Robel and Baulieu, 1994; Mellon and Vaudry, 2001). These brain-born steroids, termed neurosteroids, regulate a number of behavioral and metabolic activities, such as response to novelty, aggressiveness, anxiety, depression, stress, learning and memory, sexual behavior, locomotion, body temperature, and blood pressure (Patchev et al., 1996; Baulieu et al., 1999; Rupprecht and Holsboer, 1999; Compagnone and Mellon, 2000; Vallée et al., 2000, 2001; Bernardi et al., 2004). However, the neuronal mechanisms regulating the activity of neurosteroid-producing cells remain poorly understood.
The amphibian brain has proven to be a valuable model in which to investigate neurosteroid biosynthesis. First, several key enzymes have been localized in the frog CNS, including cytochrome P450 side-chain cleavage (P450scc) that catalyzes the conversion of cholesterol into pregnenolone (Δ5P) (Takase et al., 1999), 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (3β-HSD) that catalyzes the conversion of Δ5P into progesterone (P) (Mensah-Nyagan et al., 1994), 17β-hydroxysteroid dehydrogenase that catalyzes the conversion of androstenedione into testosterone (Mensah-Nyagan et al., 1996a,b), and hydroxysteroid sulfotransferase, which is responsible for the synthesis of biologically active sulfated steroids such as Δ5P sulfate (Δ5PS) and dehydroepiandrosterone sulfate (DHEAS) (Beaujean et al., 1999). Second, the rate of steroid biosynthesis in the frog brain is particularly high so that it is possible to study the mode of action of inhibitory neurotransmitters and neuropeptides on neurosteroid production. For instance, it has been shown that GABA, acting through GABAA receptors, inhibits the conversion of tritiated Δ5P ([3H]Δ5P) into radioactive metabolites including P, 17-hydroxypregnenolone (17OH-Δ5P), 17-hydroxyprogesterone (17OH-P), and dehydroepiandrosterone (DHEA) (Do Rego et al., 2000) and that neuropeptide Y, acting through Y1 receptors, inhibits the formation of Δ5PS and DHEAS (Beaujean et al., 2002).
In mammals, the neurohypophysial nonapeptides vasopressin (VP) and oxytocin (OT) exert a wide variety of behavioral effects, including modulation of learning and memory processes, social recognition, sexual behavior, anxiety, depression, stress responses, locomotion, and aggressiveness (Argiolas and Gessa, 1991; De Wied et al., 1993; De Wied, 1997; Everts and Koolhaas, 1997, 1999; Goodson and Bass, 2001; Hugin-Flores et al., 2003). In amphibians, vasotocin (VT), the ortholog of mammalian VP, plays a crucial role in the control of various reproductive behaviors, such as vocal communication (Boyd, 1997), appetitive responses to olfactory and visual stimuli (Iwata et al., 2000; Thompson and Moore, 2000), courtship behavior (Iwata et al., 2000), clasping behavior (Thompson and Moore, 2000, 2003), and egg-laying behavior (Moore et al., 2000). In amphibians as in mammals, the mechanism of action of neurohypophysial peptides in modulating these behaviors is still unclear. We have hypothesized that some of the central effects of VP/VT and OT/mesotocin (MT) may be mediated through modulation of neurosteroid biosynthesis.
To test this hypothesis, (1) we searched whether neurosteroid-producing neurons are innervated by VT-/MT-positive fibers, (2) we studied the effect of VT and MT on 3β-HSD and P450 17α-hydroxylase/c17, 20-lyase (P450C17) activity, and (3) we characterized the pharmacological profile of the receptors involved in the action of VT and MT on neurosteroid biosynthesis in the frog hypothalamus.
Materials and Methods
Animals.
Adult male frogs (Rana esculenta) of ∼30–40 g body weight were purchased from a commercial supplier (Couétard, Saint-Hilaire de Riez, France). The animals were housed in a temperature-controlled room (8 ± 0.5°C) under running water, on a 12 h light/dark schedule (lights on from 6:00 A.M. to 6:00 P.M.), for at least 1 week before use. To limit possible variations of neurosteroid biosynthesis attributable to circadian rhythms (Akwa et al., 1991), all animals were killed between 9:30 A.M. and 10:30 A.M. Animal manipulations were performed according to the recommendations of the French Ethical Committee and under the supervision of authorized investigators.
Chemicals and reagents.
Synthetic peptides including MT, OT, VP, VT, the V1 receptor (V1R) agonist ([Phe2, Orn8]OT), the V1b receptor (V1bR) agonist ([deamino-Cys1, β-(3-pyridyl)-d-Ala2, Arg8]VP), the V2 receptor (V2R) agonist ([deamino-Cys1, Val4, d-Arg8]VP), the V1a receptor (V1aR) antagonist ([d(CH2)51, Tyr(Me)2, Arg8]VP), the V2R antagonist ([d(CH2)51, d-Ile2, Ile4, Arg8]VP), and the OT receptor (OTR) antagonist ([d(CH2)51, Tyr(Me)2, Thr4, Orn8, des-Gly-NH29]VT) were purchased from Bachem (Bubendorf, Switzerland). The OTR agonist ([Thr4, Gly7]OT), and the V1aR antagonist ([Phe-acetyl1, O-Me-d-Tyr2, Arg6,8, Lys9]VP-NH2), synthetic androstenedione (Δ4), androsterone (An), cortisol (F), DHEA, P, testosterone (T), 11-deoxycortisol (S), 17OH-P, 17OH-Δ5P, dihydrotestosterone, 3-amino-benzoic acid ethyl ester (MS222), aminoglutethimide, ketoconazole, propylene glycol, and HEPES were purchased from Sigma (St. Louis, MO). [3H]Δ5P (specific activity, 21 Ci/mmol) was obtained from PerkinElmer (Paris, France). Dichloromethane and methanol were from Carlo Erba (Val-de-Reuil, France). Bovine serum albumin (BSA) was from Boehringer Mannheim (Paris, France). Trilostane (WIN 24.540) was a gift from Winthrop Laboratories (Clichy, France).
Antibodies and oligonucleotides.
Polyclonal antibodies raised in rabbit against human placental type I 3β-HSD (Luu-The et al., 1989) and against bovine testicular P450C17 (Tremblay et al., 1994) as well as a monoclonal antibody against VP (Robert et al., 1985) were used as primary antibodies for immunohistochemistry. Alexa 594-conjugated goat anti-rabbit γ-globulins (GARs/Alexa 594) and Alexa 488-conjugated goat anti-mouse γ-globulins (GAMs/Alexa 488), both from Invitrogen (Carlsbad, CA), were used as secondary antibodies.
Oligonucleotides were designed from partial VT receptor (VTR) and MT receptor (MTR) cDNA sequences cloned previously in the frog R. esculenta (Acharjee et al., 2004) using the following primers: VTR forward, 5′-GGC ATG TTT GCG TCT ACC TAC-3′; VTR reverse, 5′-CAG ATG TGG TGC GTT TGG GAT-3′; MTR forward, 5′-GGG ATG TTT GCT TCT ACC TAC-3′; MTR reverse, 5′-CAG ATG TGG TCA GTC TGG GAC-3′.
Immunofluorescence procedure.
Animals were anesthetized by immersion in 0.1% MS222 buffered with 0.1 m NaHCO3 to pH 7.2 and perfused transcardially with 50 ml of 0.1 m phosphate buffer (PB), pH 7.4. The perfusion was performed with 50 ml of Bouin’s fixative. The brains were dissected and postfixed in the same fixative for 24 h at 4°C. The tissues were rinsed overnight in 0.1 m PB containing 15% sucrose and then transferred into 0.1 m PB containing 30% sucrose for 24 h. The brains were placed in an embedding medium (O.C.T. Tissue Tek; Leica, Nussloch, Germany), frozen at −80°C, cut in the frontal or sagittal plane in a cryomicrotome (6-μm-thick sections), mounted on 0.5% gelatin/5% chrome alun-coated slides, and kept at 4°C until use. The tissue sections were incubated overnight at 4°C in a humid atmosphere with a mixture containing the rabbit 3β-HSD antiserum (1:100) or P450C17 antiserum (1:100) and the monoclonal VP antibody (1:1000) diluted in 0.1 m PB containing 0.3% Triton X-100 and 1% BSA. At the end of the incubation period, the sections were rinsed in three successive baths of PB and incubated for 90 min at room temperature with GAR/Alexa-594 and GAM/Alexa-488 (20 μg/ml) each. Finally, the sections were rinsed in PB and mounted with PB/glycerol (1:1).
The preparations were examined using a filter-free confocal laser scanning microscope (CLSM TCS SP2 AOBS; Leica, Heidelberg, Germany) equipped with a fluorescence DM RXA2UV optical system, and an argon UV (excitation wavelength, 405 nm), an argon (excitation wavelengths, 458, 476, 488, and 514 nm), and three helium–neon (excitation wavelengths, 543, 594, and 633 nm) ion lasers (Leica). Dual-channel confocal laser scanning microscopic (CLSM) analysis was performed using a wavelength window 600 ≤ λ ≤ 650 nm for detection of Alexa-594 and a wavelength window 500 ≤ λ ≤ 550 nm for detection of Alexa-488.
To study the specificity of the immunoreaction, the following controls were performed: (1) substitution of the 3β-HSD or P450C17 antiserum and the monoclonal VP antibody with PB; (2) incubation with nonimmune rabbit serum instead of the 3β-HSD or P450C17 antiserum; and (3) preincubation of the 3β-HSD antiserum (diluted 1:100) with purified human placental type I 3β-HSD (10−6 m) and preincubation of the monoclonal VP antibody (diluted 1:1000) with graded concentrations of synthetic VT or MT (10−10 to 10−5 m). Neuroanatomical nomenclature was based on the atlas of Neary and Northcutt (1983).
In situ hybridization histochemistry.
Adult male frogs were perfused transcardially as described above except that Bouin’s fixative was replaced by 4% paraformaldehyde. Brain slices (12-μm-thick) were cut in a cryomicrotome, mounted on 0.5% gelatin/0.05% chrome alun/0.01% poly-l-lysine-coated slides, and kept at −80°C until use. The partial VTR and MTR cDNA sequences were subcloned into the pGEM-T vector between SpeI and NcoI sites, and sense and antisense riboprobes were generated by in vitro transcription using T7 and Sp6 RNA polymerases in the presence of [35S]UTP (Combination Systems; Promega, Madison, WI). Sections were incubated for 10 min in 0.1 m triethanolamine/0.9% NaCl (pH 8.0)/0.25% acetic anhydride, rinsed in 2× SSC, and incubated for 60 min with prehybridization buffer, pH 7.5, containing 50% formamide, 0.6 m NaCl, 10 mm Tris-HCl, pH 7.5, 0.02% Ficoll, 0.02% polyvinylpyrrolidone, 0.02% BSA, 1 mm ethylene-diaminetetra-acetic acid, pH 8.0, 550 μg/ml denatured salmon sperm DNA, and 50 μg/ml yeast tRNA. Hybridization was performed overnight at 55°C in the same buffer (except for salmon sperm DNA, whose concentration was reduced to 60 μg/ml) supplemented with 10 mm dithiothreitol, 10% dextran sulfate, and 1.5 × 107 cpm/ml heat-denatured RNA riboprobes. Tissue sections were then washed in 2× SSC at 55°C and treated with ribonuclease A (50 μg/ml) for 60 min at 37°C. Five high-stringency washes were performed in 0.01× SSC containing 14 mm β-mercaptoethanol and 0.05% sodium pyrophosphate at 60°C. Tissue sections were dehydrated in ethanol and exposed onto Biomax MR Film (Eastman Kodak, Rochester, NY) for 15 d. To identify anatomical structures, slices were stained with hematoxylin and eosin.
Measurement of 3β-HSD and P450C17 activities.
Conversion of [3H]Δ5P into different metabolites, including P, 17OH-Δ5P, 17OH-P, and DHEA, was used as an index for the measurement of 3β-HSD and P450C17 activities, as described previously (Mensah-Nyagan et al., 1994). Briefly, for each experiment, the hypothalami of four frogs were rapidly dissected and each hypothalamus was cut into two slices. The tissue fragments were preincubated for 15 min in 1 ml of Ringer’s solution consisting of 15 mm HEPES buffer, 112 mm NaCl, 15 mm NaHCO3, 2 mm CaCl2, and 2 mm KCl, supplemented with 2 mg glucose/ml and 0.3 mg BSA/ml. The incubation medium was gassed with a 95% O2/5% CO2 mixture, and the pH was adjusted to 7.4. The hypothalamic fragments were incubated at 24°C for 2 h (0.5–4 h for time course experiments) in 500 μl of Ringer’s medium containing 10−6 m [3H]Δ5P and 4% propylene glycol, in the absence or presence of test substances. Aminoglutethimide and ketoconazole, which are poorly soluble in water, were dissolved in methanol (0.1%) and acetone (0.1%), respectively, and the same concentration of organic solvent was added in the control samples. At the end of the incubation period, the tissues were rinsed four times with ice-cold Ringer’s buffer, and the reaction was stopped by adding 1 ml of trichloroacetic acid. The tissues were homogenized using a glass Potter homogenizer, and the steroids were extracted three times by 1 ml of dichloromethane. The organic phase containing the steroids was evaporated under nitrogen, and the extract was redissolved in a solution consisting of 65% water/trifluoroacetic acid (TFA) (99.9:0.1; v/v; solution A) and 35% methanol/water/TFA (90:9.98:0.02; v/v/v; solution B) and prepurified on Sep-Pak C18 cartridges (Waters Associates, Milford, MA) equilibrated with a solution made of 65% solution A and 35% solution B. Steroids were eluted with 4 ml of a solution made of 10% solution A and 90% solution B. The solvent was evaporated in a Speed-Vac concentrator (Savant, Hicksville, NY), and the extracts were kept dry at 4°C until analysis.
HPLC analysis.
Sep-Pak-prepurified extracts were analyzed by reversed-phase HPLC. The HPLC equipment consisted of a Gilson (Villier-le-Bel, France) model 305 master pump acting as a system controller, a Gilson model 306 slave pump controlled by the previous pump, a Gilson model 115 variable wavelength UV detector set at 240 nm, and a Rheodyne (Rohnert Park, CA) model 7125 injector. A 0.39 × 30 cm Nova-Pak C18 column (Waters Associates) equilibrated with 60% solution A and 40% solution B was used for analysis. Each extract was injected at a flow rate of 1 ml/min and separated using a gradient of solution B (40–100% over 104 min), including four isocratic steps at 40% (0–10 min), 64% (39–59 min), 80% (69–79 min), and 100% (94–104 min) solution B. Tritiated compounds eluted from the HPLC column were detected by using a flow scintillation analyzer (Radiomatic Flo-one/Beta A-500; Packard, Meridien, CT) equipped with a Pentium III personal computer that automatically integrated the peak surface and calculated the percentage of total radioactivity contained in each peak.
Synthetic steroids used as reference standards were chromatographed under the same conditions as the tissue extracts and detected by UV absorption.
Quantification of steroid biosynthesis and statistical analysis.
The amounts of radioactive steroids formed by conversion of [3H]Δ5P were expressed as a percentage of the total radioactivity contained in all peaks resolved by HPLC, including [3H]Δ5P itself. Each value is the mean of four independent experiments. Statistical analysis was performed by ANOVA, followed by a Dunnett’s or a Bonferroni’s multiple comparison test.
Results
Immunocytochemistry
Labeling of frog brain slices with each primary antibody revealed that most of the nuclei that contain 3β-HSD- and/or P450C17-immunoreactive neurons are also innervated by VT- and/or MT-containing fibers, i.e., the anterior commissure, the lateral and medial amygdala, the anterior preoptic area, the dorsal and ventral hypothalamic nuclei, the suprachiasmatic nucleus, the posterior tuberculum, the nucleus of the periventricular organ, and the ventral part of the magnocellular preoptic nucleus (Table 1).
Comparative distribution and relative abundance of 3β -HSD- and P450C17-positive neurons, VT- and MT-immunoreactive fibers, and VTR and MTR mRNAs in the telencephalon and diencephalon of the frog R. esculenta
Double labeling of frog diencephalic slices with the rabbit 3β-HSD antiserum and the monoclonal VP antibody, combined with dual-channel CLSM analysis, showed that many 3β-HSD-positive cell bodies are surrounded by bundles of beaded fibers containing VP-like immunoreactivity in the anterior preoptic area (Fig. 1A–C) and the posterior tuberculum (Fig. 1D–F). At a higher magnification, VP-immunoreactive fibers appeared to contact 3β-HSD-containing neurons (Fig. 1, insets). Similarly, in the dorsal and ventral hypothalamic nuclei, the nucleus of the periventricular organ, and the suprachiasmatic nucleus, 3β-HSD-positive perikarya were surrounded by a network of VP-immunoreactive processes (data not shown). Double labeling of brain sections with the rabbit P450C17 antiserum and the monoclonal VP antibody revealed the existence of varicose VP-immunoreactive processes next to P450C17-positive cell bodies in the anterior preoptic area (Fig. 2A–C) and in the medial amygdala (Fig. 2D–F). At a higher magnification, VP-immunoreactive fibers were seen in the close vicinity of P450C17-positive cells (Fig. 2, insets). P450C17-positive neurons surrounded by VP-immunoreactive nerve fibers were also observed in the lateral amygdala, the ventral part of the magnocellular preoptic nucleus, the nucleus of the periventricular organ, the posterior tuberculum, and the dorsal and ventral hypothalamic nuclei (data not shown).
Dual-channel confocal laser scanning microscope photomicrographs comparing the distribution of 3β-HSD- and VP-like immunoreactivity in the anterior preoptic area (Poa; A–C) and in the posterior tuberculum (PT; D–F). Frontal brain sections were labeled with the rabbit antiserum against 3β-HSD revealed with GAR/Alexa-594 (A, D) and the monoclonal antibody against VP revealed with GAM/Alexa-488 (B, E). Combination of the two images acquired in A and B and in D and E revealed that the 3β-HSD-positive cells in the anterior preoptic area and the posterior tuberculum are contacted by VT-immunoreactive fibers (C, F). The insets show higher-magnification views of VT-immunoreactive nerve fibers in the close vicinity of 3β-HSD-positive neurons (C, F). Scale bars, 10 μm.
Dual-channel confocal laser scanning microscope photomicrographs comparing the distribution of P450C17- and VP-like immunoreactivity in the anterior preoptic area (Poa; A–C) and in the medial amygdala (MA; D–F). Frontal brain sections were labeled with the rabbit antiserum against P450C17 revealed with GAR/Alexa-594 (A, D) and the monoclonal antibody against VP revealed with GAM/Alexa-488 (B, E). Combination of the two images acquired in A and B and in D and E revealed that the P450C17-positive cells in the anterior preoptic area and the medial amygdala are contacted by VT-immunoreactive fibers (C, F). The insets show higher-magnification views of VT-immunoreactive nerve fibers in the close vicinity of P450C17-positive neurons (C, F). Scale bars, 10 μm.
Preincubation of the 3β-HSD antiserum with purified human type I 3β-HSD (10−6 m) totally abolished immunostaining (data not shown). Similarly, preincubation of the monoclonal VP antibody with graded concentrations of synthetic VT and MT (10−10 to 10−5 m) resulted in a dose-dependent decrease of the immunoreaction, with total extinction at concentrations of 10−7 m VT and 10−5 m MT (Table 2, Fig. 3). No fluorescence was observed when the primary antisera against 3β-HSD or P450C17 were substituted with nonimmune rabbit serum or when the VP antibody was substituted with PB (data not shown).
Specificity control of the vasotocin/mesotocin immunostaining
Labeling of consecutive frontal sections through the anterior preoptic area (Poa) showing the specificity control of the immunoreaction. Adjacent sections were incubated with the VP antibody (A, C) or with the antibody preincubated with 10−7 m synthetic VT (B) or 10−5 m MT (D). Scale bars, 100 μm.
Distribution of VT and MT receptors in the diencephalon
The localization of VTR and MTR mRNAs in the frog diencephalon was determined by in situ hybridization histochemistry (Fig. 4). A high concentration of VTR mRNA was observed in the anterior preoptic area and the lateral amygdala (Fig. 4A), as well as in the posterior tuberculum, the nucleus of the periventricular organ, the posterior thalamic nucleus, and the dorsal and ventral hypothalamic nuclei (Fig. 4B). A moderate density of VTR mRNA was detected in the pallium and the medial amygdala (Fig. 4A).
In situ hybridization analysis showing the distribution of VTR mRNA (A–C) and MTR mRNA (D–F) in the frog diencephalon. Frontal brain sections were hybridized with the antisense VTR riboprobe (A, B) or the antisense MTR riboprobe (D, E). Consecutive sections to those shown in A and E were hybridized with the sense VTR riboprobe (C) or the sense MTR riboprobe (F). The anatomical distributions of 3β-HSD-positive cell bodies (blue asterisks) and P450C17-positive cell bodies (red asterisks) as well as the designation of the anatomical structures are indicated on the right hemisections. For abbreviations, see Table 1. Scale bar, 500 μm.
A high concentration of MTR mRNA was found in all subdivisions of the pallium, the anterior commissure, the lateral and medial amygdala, the anterior preoptic area (Fig. 4D), the posterior thalamic nucleus, the posterior tuberculum, the nucleus of the periventricular organ, and the dorsal and ventral hypothalamic nuclei (Fig. 4E). A moderate density of MTR mRNA was seen in the anterior commissure, the dorsal pallium, the lateral septum, the nucleus accumbens (Fig. 4D), the central thalamic nucleus, and the ventromedial, dorsal, and ventral thalamic nuclei (Fig. 4E). Control sections incubated with the sense VTR (Fig. 4C) and MTR (Fig. 4F) probes exhibited only weak background staining.
A comparison of the distribution and relative density of VTR and MTR mRNAs to those of 3β-HSD-immunoreactive neurons described previously (Mensah-Nyagan et al., 1994) and P450C17-containing neurons (our unpublished data) in the frog telencephalon and diencephalon revealed that most nuclei that contain 3β-HSD- and P450C17-positive neurons are enriched with VTR and MTR mRNAs, notably the anterior preoptic area, the lateral and medial amygdala, the posterior tuberculum, the nucleus of the periventricular organ, and the dorsal and ventral hypothalamic nuclei (Table 1, Fig. 4). In contrast, 3β-HSD- and P450C17-immunoreactive cell bodies were not found in several telencephalic and diencephalic nuclei in which VTR and MTR mRNAs are located, such as the dorsal and ventral striatum, the lateral septum, the nucleus accumbens, and the central, posterior, and ventral thalamic nuclei (Table 1).
Effects of VT and MT on neurosteroid biosynthesis
After a 2 h incubation of frog hypothalamic explants with [3H]Δ5P, reversed-phase HPLC analysis of the tissue extracts made it possible to resolve several radioactive compounds, including 17OH-Δ5P, An, DHEA, Δ4, T, 17OH-P, and P (Fig. 5A). Addition of VT (10−7 m) to the incubation medium markedly stimulated the conversion of [3H]Δ5P into radioactive steroids (Fig. 5B). Exposure of frog hypothalamic explants to graded concentrations of VT or MT (10−10 to 10−5 m) induced a dose-dependent increase of P, 17OH-Δ5P, 17OH-P, and DHEA biosynthesis (Fig. 6). Maximum stimulation was observed at a concentration of 10−6 m. Administration of graded concentrations of the mammalian nonapeptides VP and OT also provoked a dose-dependent stimulation of P, 17OH-Δ5P, 17OH-P, and DHEA production (Fig. 6).
HPLC analysis of radioactive steroids extracted from frog hypothalamic explants after a 2 h incubation with [3H]Δ5P in control conditions (A) or in the presence of 10−7 m VT (B). The ordinate axis indicates the radioactivity measured in the HPLC eluent. The dashed lines represent the gradient of secondary solvent (percentage solution B). The arrows indicate the elution positions of standard steroids.
Effects of graded concentrations of VT (•), MT (▪), VP (▵), and OT (▾) on the conversion of tritiated pregnenolone into P, 17OH-Δ5P, 17OH-P, and DHEA by frog hypothalamic explants. The values were calculated from the areas under the peaks in chromatograms similar to those presented in Figure 5. Results are expressed as percentages of the amount of each steroid formed in the absence of peptides. Values are the mean ± SEM of four independent experiments.
Time course experiments revealed that VT and MT induced a significant stimulation of neurosteroid biosynthesis within 30 min and the maximum response was observed after 2 h; then, the stimulatory effect of VT and MT gradually declined during the next 2 h (Fig. 7).
Time course of the conversion of [3H]Δ5P into radioactive P, 17OH-Δ5P, 17OH-P, and DHEA by frog hypothalamic explants in the absence (○) or presence (•) of 10−7 m vasotocin or 10−7 m mesotocin (▪). The values were calculated from the areas under the peaks in chromatograms similar to those presented in Figure 5. Results are expressed as percentages of the amount of each steroid formed compared with the total amount of radiolabeled compounds resolved by HPLC analysis, including [3H]Δ5P. Values are the mean ± SEM of four independent experiments.**p < 0.01; ***p < 0.001 compared with respective control values (one-way ANOVA followed by a post hoc Dunnett’s test).
Exposure of frog hypothalamic explants to aminoglutethimide (10−4 m), a selective inhibitor of cytochrome P450scc, did not significantly affect the spontaneous conversion of Δ5P into P and 17OH-Δ5P (Fig. 8). Aminoglutethimide did not significantly modify the effect of VT and MT on 17OH-Δ5P production but significantly (p < 0.01) enhanced the stimulatory effect of VT and MT on P formation (Fig. 8). Incubation of frog hypothalamic slices with trilostane (10−4 m), a specific inhibitor of 3β-HSD, and ketoconazole (10−4 m), an inhibitor of P450C17, markedly inhibited the spontaneous formation of P and 17OH-Δ5P and totally suppressed the stimulatory effect of VT and MT on P and 17OH-Δ5P biosynthesis (Fig. 8).
Effects of VT (10−7 m) and MT (10−7 m) in the absence or presence of the cytochrome P450scc inhibitor aminoglutethimide (10−4 m), and the 3β-HSD inhibitor trilostane (10−4 m) and cytochrome P450C17 inhibitor ketoconazole (10−4 m) on the conversion of tritiated pregnenolone into P and 17OH-Δ5P by frog hypothalamic explants. The values were calculated from the areas under the peaks in chromatograms similar to those presented in Figure 5. Results are expressed as percentages of the amount of each steroid formed in the absence of drugs (control). Each value is the mean ± SEM of four independent experiments. ***p < 0.001 versus control; ##p < 0.01, ###p < 0.001 versus VT- or MT-stimulated levels; NS, not statistically different from control; ns, not statistically different from VT- or MT-stimulated levels (one-way ANOVA followed by a post hoc Bonferroni’s test).
Pharmacological characterization of the receptor mediating the actions of VT and MT
To determine the pharmacological profile of the receptors involved in the stimulatory actions of VT and MT, frog hypothalamic explants were incubated with various VP and OT receptor agonists and antagonists. Administration of the mutual V1aR/V1bR agonist (1) [Phe2, Orn8]OT (10−7 m) or the selective V1bR agonist (2) [deamino-Cys1, β-(3-pyridyl)-d-Ala2, Arg8]VP (10−7 m) both mimicked the stimulatory effect of VT on the conversion of [3H]Δ5P into 17OH-Δ5P and P (Fig. 9). Conversely, the selective V2R agonist (3) [deamino-Cys1, Val4, d-Arg8]VP (10−7 m) did not significantly modify the formation of 17OH-Δ5P and P by diencephalic explants (Fig. 9). The stimulatory effect of VT on the biosynthesis of 17OH-Δ5P and P was completely suppressed by the selective V1aR antagonists (4) [d(CH2)51, Tyr(Me)2, Arg8]VP (10−6 m) and (5) [Phe-acetyl1, O-Me-d-Tyr2, Arg6,8, Lys9]VP-NH2 (10−6 m) (Fig. 10). In contrast, the V2R antagonist (6) [d(CH2)51, d-Ile2, Ile4, Arg8]VP (10−6 m) and the OTR antagonist (7) [d(CH2)51, Tyr(Me)2, Thr4, Orn8, des-Gly-NH29]VT (10−6 m) did not significantly affect VT-induced stimulation of 17OH-Δ5P and P synthesis (Fig. 10). Concurrently, the stimulatory effect of MT on the production of 17OH-Δ5P and P was not mimicked by the OTR agonist (8) [Thr4, Gly7]OT (10−7 m) but was markedly reduced by both the selective V1aR antagonist (4) [d(CH2)51, Tyr(Me)2, Arg8]VP (10−6 m) and the specific OTR antagonist (7) [d(CH2)51, Tyr(Me)2, Thr4, Orn8, des-Gly-NH29]VT (10−6 m each) (Fig. 11). Finally, MT-induced production of 17OH-Δ5P and P was not affected by the specific V2R antagonist (6) [d(CH2)51, d-Ile2, Ile4, Arg8]VP (10−6 m) (Fig. 11).
Effects of VT and various agonists (10−7 m each) on the conversion of tritiated pregnenolone into P and 17OH-Δ5P by frog hypothalamic explants. 1, [Phe2, Orn8]OT, V1aR/V1bR agonist; 2, [deamino-Cys1, β-(3-pyridyl)-d-Ala2, Arg8]VP, selective V1bR agonist; 3, [deamino-Cys1, Val4, d-Arg8]VP, V2R agonist. The values were calculated from the areas under the peaks in chromatograms similar to those presented in Figure 5. Results are expressed as percentages of the amount of each steroid formed in the absence of peptide (control). Values are the mean ± SEM of four independent experiments. **p < 0.01; ***p < 0.001; NS, not statistically different from control (one-way ANOVA followed by a post hoc Dunnett’s test).
Effects of VT (10−7 m) in the absence or presence of various antagonists (10−6 m each) on the conversion of tritiated pregnenolone into P and 17OH-Δ5P by frog hypothalamic explants. 4, [d(CH2)51, Tyr(Me)2, Arg8]VP, V1aR antagonist; 5, [Phe-acetyl1, O-Me-d-Tyr2, Arg6,8, Lys9]VP-NH2, V1aR antagonist; 6, [d(CH2)51, d-Ile2, Ile4, Arg8]VP, V2R antagonist; 7, [d(CH2)51, Tyr(Me)2, Thr4, Orn8, des-Gly-NH29]VT, OTR antagonist. The values were calculated from the areas under the peaks in chromatograms similar to those presented in Figure 5. Results are expressed as percentages of the amount of each steroid formed in the absence of peptide (control). Values are the mean ± SEM of four independent experiments. *p < 0.05, ***p < 0.001 versus control; ##p < 0.01, ###p < 0.001 versus VT-stimulated levels; NS, not statistically different from control; ns, not statistically different from VT-stimulated levels (one-way ANOVA followed by a post hoc Bonferroni’s test).
Effects of MT (10−7 m) in the absence or presence of various antagonists (10−6 m each) and an OTR agonist (10−7 m) on the conversion of tritiated pregnenolone into P and 17OH-Δ5P by frog hypothalamic explants. 4, [d(CH2)51, Tyr(Me)2, Arg8]VP, V1aR antagonist; 6, [d(CH2)51, d-Ile2, Ile4, Arg8]VP, V2R antagonist; 7, [d(CH2)51, Tyr(Me)2, Thr4, Orn8, des-Gly-NH29]VT, OTR antagonist; 8, [Thr4, Gly7]OT, OTR agonist. The values were calculated from the areas under the peaks in chromatograms similar to those presented in Figure 5. Results are expressed as percentages of the amount of each steroid formed in the absence of peptide (control). Values are the mean ± SEM of four independent experiments. ***p < 0.001 versus control; #p < 0.05, ###p < 0.001 vs MT-stimulated levels; NS, not statistically different from control; ns, not statistically different from MT-stimulated levels (one-way ANOVA followed by a post hoc Bonferroni’s test).
Discussion
Neurosteroids exert a large array of neurophysiological and behavioral activities, suggesting that the biosynthesis of steroids in the brain must be tightly controlled. However, to date, little is known regarding the neuronal mechanisms regulating the activity of neurosteroid-producing cells. The present study has shown that, in the frog diencephalon, neurons expressing the steroidogenic enzymes 3β-HSD and P450C17 are contacted by VT/MT-positive fibers and that VT and MT stimulate the production of Δ4-3-keto- and Δ5-3β-hydroxy-neurosteroids.
Anatomical relationship between neurosteroid-producing neurons, VTergic/MTergic fibers, and VT/MT receptors
The neuronal populations containing 3β-HSD and VT/MT immunoreactivities have been mapped previously in the brain of the European green frog R. esculenta/ridibunda (González and Smeets, 1992; Mensah-Nyagan et al., 1994; Smeets and González, 2001). A comparison of these two neuronal systems has revealed that the diencephalic nuclei containing 3β-HSD-expressing neurons also exhibit a high density of VT/MT-positive fibers and terminals (Table 1). Likewise, the magnocellular preoptic nucleus, which contains a population of P450C17-expressing neurons, is innervated by VTergic/MTergic fibers (Table 1). Using a monoclonal antibody against VP (Robert et al., 1985) that has high affinity for VT and cross-reacts to a certain extent with MT, we now show that VT- and possibly MT-immunoreactive fibers are found in the close proximity of neurons expressing 3β-HSD and P450C17. Interestingly, in the quail hypothalamus, aromatase-containing cell bodies, which are responsible for the production of neuroestrogens, are also innervated by VTergic fibers (Viglietti-Panzica et al., 1994; Balthazart et al., 1997).
We recently cloned a V1a-type VTR and the MTR in R. esculenta (Acharjee et al., 2004), so that we are now able to compare the distribution of the mRNAs encoding these two receptors with the localization of steroidogenic neurons in the frog diencephalon (Table 1). Intense expression of VTR and/or MTR mRNAs was seen in most hypothalamic nuclei that contain 3β-HSD- and/or P450C17-positive neurons. Together, these neuroanatomical data prompted us to investigate the possible effects of VT and MT on neurosteroid biosynthesis in frog.
Effect of VT and MT on neurosteroid biosynthesis
Using a pulse-chase approach, we show that VT and MT, as well as their mammalian counterparts VP and OT, induce a concentration-dependent stimulation of the formation of Δ4-3-keto-steroids, including P and 17OH-P, and Δ5-3β-hydroxysteroids, including 17OH-Δ5P and DHEA. The stimulatory effect of VT and MT on P and 17OH-Δ5P production was suppressed by the 3β-HSD inhibitor trilostane and the P450C17 inhibitor ketoconazole. These data provide the first evidence that peptides of the VP family activate neural cells that express 3β-HSD and P450C17. Worthy of note, it has been shown previously that VP-related peptides can regulate the activity of peripheral steroidogenic organs; in particular, the secretion of corticosteroids is stimulated by VP in rat and human (Hinson et al., 1987; Perraudin et al., 1993; Guillon et al., 1995) and by VT in frog (Larcher et al., 1989).
Time course experiments revealed that a 30 min incubation of hypothalamic explants with VT or MT was sufficient to induce a robust increase in neurosteroid production and that the maximum effect was observed after a 2 h exposure to VT or MT. This rapid response strongly suggests that VT and MT activate steroidogenic enzymes at a posttranslational level, possibly through serine (Ser) and/or threonine (Thr) phosphorylation. Consistent with this notion, it is now established that phosphorylation of Ser106 and Thr112 residues in human P450C17 stimulates the activity of the enzyme (Lin et al., 1993; Zhang et al., 1995; Miller et al., 1997; Pandey and Miller, 2005). Rapid changes in the activity of steroidogenic enzymes in nervous tissue has been reported previously. In particular, kainate, AMPA, and dopaminergic agonists have been shown to induce modifications of aromatase activity within minutes in quail hypothalamic explants (Balthazart et al., 2002, 2006). Similarly, a 30 min exposure of isolated rat retina to NMDA stimulates the production of Δ5P and Δ5PS (Guarneri et al., 1998). In vivo treatment of rat with the dopamine D4 antagonist clozapine induces a rapid increase in the concentration of P, tetrahydroprogesterone, and tetrahydrodeoxycorticosterone in the cerebral cortex and striatum (Barbaccia et al., 2001). These observations, together with the present findings, are consistent with a posttranslational regulation of brain steroidogenic enzymes by neurotransmitters and neuropeptides. We cannot exclude, however, that VT and MT could also activate the expression of 3β-HSD and/or P450C17 genes because a stimulatory effect was still observed 4 h after the onset of incubation with each of these peptides.
To investigate the possible interference of endogenous Δ5P in the conversion of [3H]Δ5P into tritiated neurosteroids, pulse-chase experiments were also performed in the presence of aminoglutethimide, a specific inhibitor of the cholesterol side-chain cleavage enzyme P450scc. The fact that aminoglutethimide did not affect the basal production of P and 17OH-Δ5P indicates that the stimulatory effect of VT and MT cannot be accounted for by blockage of P biosynthesis. However, we found that aminoglutethimide slightly enhanced the stimulatory effect of VT and MT on P production, suggesting that endogenous Δ5P may compete with the tritiated precursor, leading to an attenuation of the steroidogenic response to VT and MT.
Pharmacological characterization of VT and MT receptors
To determine the pharmacological profile of the receptors mediating the action of VT and MT on steroidogenic neurons, we used various peptidergic agonists and antagonists that have been developed for the study of mammalian VP/OT receptors (Manning and Sawyer, 1993). The stimulatory effect of VT was mimicked by the V1R agonist [Phe2, Orn8]OT (Manning and Sawyer, 1984) and completely suppressed by the selective V1aR antagonists [d(CH2)51, Tyr(Me)2, Arg8]VP (Kruszynski et al., 1980) and [Phe-acetyl1, O-Me-d-Tyr2, Arg6,8, Lys9]VP-NH2 (Howl et al., 1993). In contrast, the V2R agonist [deamino-Cys1, Val4, d-Arg8]VP (Manning and Sawyer, 1984), and the V2R antagonist [d(CH2)51, d-Ile2, Ile4, Arg8]VP (Manning et al., 1984) and the OTR antagonist [d(CH2)51, Tyr (Me)2, Thr4, Orn8, des-Gly-NH29]VT (Manning and Sawyer, 1989) did not affect, respectively, spontaneous and VT-induced neurosteroid production. These observations, together with the expression of V1aR mRNA in the hypothalamic nuclei containing 3β-HSD/P450C17-immunoreactive neurons strongly suggest that the effect of VT on neurosteroid biosynthesis is mediated through a V1a-like receptor. Conversely, the stimulatory effect of MT was blocked by the OTR antagonist [d(CH2)51, Tyr (Me)2, Thr4, Orn8, des-Gly-NH29]VT, but the V1aR antagonist [d(CH2)51, Tyr(Me)2, Arg8]VP also blocked the response, and the OTR agonist [Thr4, Gly7]OT (Richard et al., 1991) was inactive. However, the fact that MT is ∼30 times more potent on MTR-transfected cells than on VT1R- or V1aR-transfected cells (Acharjee et al., 2004) supports the notion that the effect of MT on neurosteroidogenesis is actually mediated through MTR. Furthermore, the V1b agonist [deamino-Cys1, β-(3-pyridyl)-d-Ala2, Arg8]VP (Schwartz et al., 1991) provoked a significant stimulation of neurosteroid formation. Whether this effect should be ascribed to nonselective activation of the frog V1aR or to selective interaction with a V1bR remains to be determined. These data illustrate the limitation of using selective ligands designed for mammalian receptors to characterize the homologous receptors in nonmammalian species.
Functional significance
A number of studies have shown that central or peripheral injections of VT have profound effects on reproductive behaviors in amphibians (for review, see Moore et al., 1994; Iwata et al., 2000; Woolley et al., 2004). In particular, VT stimulates vocalization in green treefrogs (Penna et al., 1992), bullfrogs (Boyd, 1994), cricket frogs (Marler et al., 1995; Chu et al., 1998), great plain toads (Propper and Dixon, 1997), and gray treefrogs (Semsar et al., 1998; Klomberg and Marler, 2000). Intracerebroventricular injection of VT also stimulates male courtship behavior, male amplectic behavior, and female sexual receptivity (Moore and Miller, 1983; Boyd, 1997; Klomberg and Marler, 2000; Thompson and Moore, 2000).
Concurrently, several reports indicate that neuroactive steroids regulate reproductive behaviors in amphibians (for review, see Moore et al., 2005). In particular, corticosterone inhibits mating and courtship behaviors (Moore and Miller, 1984), and testosterone facilitates clasping behavior (Moore and Rose, 2002) in male rough-skinned newts, and adrogens modulate vocalizations and advertisement callings in South African clawed frogs (Wetzel and Kelley, 1983; Kelley and Tobias, 1999). These observations together with the present findings strongly suggest that some of the behavioral effects of VP/VT and OT/MT could be mediated through modulation of neurosteroid-producing cell activity. In support of this hypothesis, several nuclei containing steroidogenic neurons in the frog telencephalon and diencephalon, including the lateral and medial amygdala, the anterior preoptic area, and the ventral hypothalamic nucleus are clearly involved in the regulation of male calling behavior (Boyd, 1997) and reproductive behavior (Zoeller and Moore, 1988; Moore et al., 2000) in amphibians.
In conclusion, the present study has provided the first evidence for neuroanatomical relationships between VTergic neurons and neurosteroid-secreting cells in the CNS of vertebrates. Our data demonstrate that VT and/or MT stimulate the biosynthesis of Δ4-3-keto-neurosteroids and Δ5-3β-hydroxy-neurosteroids, suggesting that some of the neurophysiological actions of VT and MT could be mediated through modulation of the activity of 3β-HSD- and P450C17-expressing neurons.
Footnotes
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This work was supported by grants from the Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 413, a France–Québec exchange program (INSERM–Le Fonds de la Recherche en Santé de Québec; to G.P., H.V.), a France–Korean exchange program (INSERM–Korea Science and Engineering Foundation and Science and Technology Amicable Research; to J.Y.S., H.B.K., H.V.), the Plate-Forme Régionale de Recherche en Imagerie Cellulaire, and the Conseil Régional de Haute-Normandie. We thank Colette Piard for skillful technical assistance.
- Correspondence should be addressed to Dr. Hubert Vaudry, European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, Institut National de la Santé et de la Recherche Médicale Unité 413, UA Centre National de la Recherche Scientifique, University of Rouen, 76821 Mont-Saint-Aignan, France. Email: hubert.vaudry{at}univ-rouen.fr
