Figure 2.
WT-like FTC axons are present in mice lacking ephrin-B3 or EphB receptors. A–D, FTC projections visualized through focal applications of DiI in open-book spinal cord preparations derived from E12.5–E13.5 WT, ephrin-B3−/−, EphB1−/−B2+/−B3−/−, and EphB1−/−B2−/−B3−/− mouse embryos. The labeled cohorts contain representative examples of appropriately shaped FTC projections in both WT and mutant mice. Although these examples contain multiple FTC axons, an embryo was classified as containing an FTC projection if it displayed at least one transversely oriented axon. E, FTC projections were more frequently observed in mice lacking ephrin-B3 or multiple EphB receptors. *p < 0.05, **p < 0.001 (Fisher’s exact test). Thin white lines demarcate the lateral edges of the FP. Scale bar, 100 μm.