Figure 5.
wrd is required postsynaptically for synaptic function.
a
, Representative traces from wild type (elav Gal4/+), wrd104
hz (elav Gal4/+; wrd104
/wrd104
), and muscle rescue (G7 Gal4/UAS wrd; wrd104
/wrd104
). Calibration: 10 ms, 10 mV for evoked release; 250 ms, 2 mV for spontaneous release.
b–d
, The mean mEJP amplitude, EJP amplitude, and quantal content are plotted for wild type (elav Gal4/+), wrd104
hz. (elav Gal4/+; wrd104
/wrd104
), wrd104
/Df(3R)DG4 (elav Gal4/+; wrd104
/Df(3R)DG4), neuronal Wrd (elav Gal4/UAS wrd), Wrd neuronal rescue (elav Gal4/UAS wrd; wrd104
/wrd104
), muscle Wrd (G7 Gal4/UAS wrd), and Wrd muscle rescue (G7 Gal4/UAS wrd; wrd104
/wrd104
) (n = 8 for wild type and wrd104
/Df(3R)DG4; n = 9 for wrd104
hz and neuronal Wrd; n = 6 for Wrd neuronal rescue; n = 12 for muscle Wrd; and n = 10 for Wrd muscle rescue). mEJPs are not significantly different for any of the genotypes compared with wild type (wrd104/Df(3R)DG4, p > 0.3; all others p > 0.9). Mean EJP value and quantal content are decreased by 56 and 51%, respectively, in wrd104
hz mutants, with similar decreases observed in wrd104
/Df(3R)DG4 [wrd104
hz and wrd104
/Df(3R)DG4 are not significantly different; p > 0.2 (EJP) and p > 0.3 (quantal content)]. Muscle expression of Wrd in the wrd104
hz mutants rescues both mean EJP and quantal content to wild-type levels [wrd104
hz vs muscle rescue, p < 0.05 (EJP) and p < 0.001 (quantal content); muscle rescue vs WT, p > 0.8 (EJP) and p > 0.3 (quantal content)]. *p < 0.01 and **p < 0.001 for mean EJP relative to wild type in
c
. In
d
, *p < 0.05, **p < 0.01, and ***p < 0.001 for mean quantal content relative to wild type. Error bars represent SEM.
e
, Wrd staining of NMJ in Wrd muscle rescue (G7 Gal4/UAS wrd; wrd104
/wrd104
) demonstrates localization of Wrd at the NMJ when expressed in muscle in the absence of endogenous Wrd. Inset shows similar staining pattern with Dlg, a primarily postsynaptic marker.