Figure 2.
Figure 2.
Axon formation was reversibly blocked by JNK inhibition. a, b, Control (CTL) neurons at 24 and 48 h in culture. c, d, Neurons chronically treated with the JNK inhibitor SP600125. e, Neurons incubated with SP600125 for 48 h, followed by washout for 24 h. f, Quantification of axon-bearing neurons under control conditions (white bars), after chronic SP600125 treatment (black bars), or with SP600125 washout (gray bar). Axons from individual cells could not be accurately quantified in 72 h control cultures, because the cultures had formed too dense of a network. Error bars indicate SEM. ∗∗∗∗p < 0.0001. Scale bars, 25 μm. SP, SP600125.
