Activated c-Jun N-Terminal Kinase Is Required for Axon Formation

Molecular inhibition of JNK interferes with axonogenesis. a, Representative neurons at 2 d in culture from a coculture of neurons separately transfected to express YFP–JBD (a dominant-negative inhibitor of JNK) or soluble CFP (controls). b, Quantification of average neurite lengths for neurons either expressing YFP–JBD (filled bars; n = 91 neurons, 520 processes over 3 independent experiments) or soluble CFP (open bars; n = 92 neurons, 407 processes) at 2 d in culture. Scale bar, 50 μm. ∗∗∗p < 0.001. n.s., No statistical significance. Error bars indicate SEM.