Figure 1.
Depolarization of SCs and BCs suppresses parallel fiber synapses by activating cannabinoid receptors. A, D, G, Fluorescent images of a PC (A), an SC (D), and a BC (G), obtained with a two-photon laser scanning microscope, are shown. Images have been inverted. PFs were stimulated with an extracellular electrode at 0.5 Hz, and EPSCs were recorded in voltage clamp at a holding potential of −70 mV. At time t = 0, cells were depolarized to 0 mV for 2 s. B, E, H, Average responses of representative experiments are shown before and 3 s after the beginning of the postsynaptic depolarization for control conditions (left) and in the presence of the CB1R antagonist AM251 (2 μm) (right) for PCs (B), SCs (E), and BCs (H). C, F, I, Summaries of the DSE time course are shown for control conditions (black symbols) and in the presence of AM251 (open symbols) for PCs (C), SCs (F), and BCs (I). Pre, Pre-pulse; norm., normalized.
