Figure 1.
Enhanced vulnerability and apoptosis-like features of sublethally stretched neurons in response to NMDA. A, Neuronal death 20 h after the indicated insults as assessed by propidium iodide uptake. Where used, NMDA was applied for 1 h after the stretch. Asterisk, Difference from 30 μm NMDA (*p < 0.05; n = 4). B, Staurosporine and stretch plus 30 μm NMDA, but not stretch alone, induces internucleosomal DNA laddering (arrowheads) after insult. Data are representative of three separate experiments. C, Staurosporine and stretch plus 30 μm NMDA, but not HEPES-treated controls, enhances TUNEL (green) labeling of the cultures. Cultures were costained with Hoescht 33258 to illustrate cell nuclei (red). Images are representative of three separate experiments. Scale bar, 200 μm.
