Uniform Serial Sectioning for Transmission Electron Microscopy

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E. Perry's present address: Department of Cellular Biology and Anatomy, Electron Microscopy Core, Medical College of Georgia, Augusta, GA 30912-2630.
M. Feinberg's present address: Department of Health Sciences, Boston University, Boston, MA 02118.
L. Ostroff's present address: Center for Neural Sciences, New York University, New York, NY 10003.

This work was supported by National Institutes of Health Grants NS21184, NS33574, and EB002170 (K.M.H.).Serial sectioning was first learned from a fellow student, Barbara McGuire, when K.M.H. was a student in the Neurobiology Course at the Marine Biological Laboratory, Woods Hole (1980; Woods Hole, MA) and evolved during a postdoctoral fellowship with Dennis Landis and John Stevens. The methods were refined with the help of research assistants and students while K.M.H. was on the faculty of Harvard Medical School at Children's Hospital (Boston, MA), Boston University (Boston, MA), and the Medical College of Georgia (Augusta, GA). Input from numerous collaborators and colleagues has been helpful to delineate reliable methods; in particular we thank Josef Spacek, Joanne Buchanan, and Thomas Reese. We also thank Robert Smith for help preparing the figures and supplemental movies.
Editor's Note: Toolboxes are a new, occasional feature in the Journal designed to briefly highlight a new method or a resource of general use in neuroscience or to critically analyze existing approaches or methods. For more information, see http://www.jneurosci.org/itoa.shtml.
Correspondence should be addressed to Kristen M. Harris, Section of Neurobiology and Center for Learning and Memory, The University of Texas at Austin, 1 University Station Stop C7000, Austin, TX 78712-0805. kharris{at}mail.clm.utexas.edu

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