Figure 2.
Kv4.2 clusters are expressed in pyramidal cell somata, dendrites, and spines. A, Confocal microscopic images of punctate Kv4.2 immunolabeling (red) in layers 4 and 5 of mouse primary visual cortex. The distribution of Kv4.2 clusters is non-uniform, showing a higher density over the neuropil than the pyramidal cell soma in the same focal plane (arrows; A, B). B, C, Kv4.2 clusters (red, yellow) are associated with YFP-labeled (green) pyramidal cell somata and with basal and apical dendrites. D–F, High-magnification images of the boxed apical dendrite of a YFP-labeled layer 5 pyramidal neuron (B, C), showing close association of Kv4.2-labeled clusters (red and yellow puncta marked with arrows) with dendritic shaft and spines. The numbers indicate layers. Scale bars: A–C, 10 μm; D–F, 2 μm.