Figure 3.
Expression profile of interneuron markers in EGFP-positive and ChAT-IR neurons. A, B, EGFP-positive (A) and ChAT-IR (B) neurons coexpress calretinin (CR) (A', B'). C, D, EGFP-positive (C) and ChAT-IR (D) neurons are calbindin negative (CB) (C', D'). A“–D”, Overlay. E, The graph shows that most EGFP-positive (green bars) and ChAT-IR (red bars) are VIP-IR and calretinin-IR, but there is little or no coexpression with somatostatin, CCK, calbindin, and parvalbumin. Scale bars, 50 μm.