Functional Characterization of Intrinsic Cholinergic Interneurons in the Cortex

Differential coexpression of GAD67 in bipolar and multipolar EGFP-positive neurons. A–D, EGFP immunohistochemistry (A, B) combined with in situ hybridization for GAD67 (C, D). Bipolar EGFP-positive cells are GAD67 negative, and multipolar EGFP-positive cells are GAD67 positive. Scale bars, 20 μm. E, Quantitative evaluation.