Figure 6.
A, Estradiol-induced increase in synaptic cluster density is inhibited in neuron cultures pretreated with ICI 182,780 (1 μm), an ER antagonist, before estradiol exposure. Scale bar, 10 μm. B, Quantification of NR1 (green) and vGlut1 (red) colocalization in cultures treated with ICI before estradiol were no different than control levels. Cultures exposed to ICI alone, without any estradiol, were also no different than control levels. *, Significant difference from control; #, significant difference from estradiol and ICI treated; @, significant difference from ICI-only treated (p < 0.01, ANOVA with Newman–Keuls post hoc; n = 18–29). All values are mean ± SEM. E, Estradiol; ICI, ICI 182,780.