Article Figures & Data
Figures
- Figure 1.
Acute stress facilitates mGluR-LTD in the rat hippocampal CA1 region. A, fEPSP responses to increasing stimulus intensities do not differ between slices from control and stressed rats. B, A 10 min application of 50 μm DHPG elicits LTD in slices from stressed but not from control rats. Whether analyzed during the last 10 or 20 min of recordings, stress, but not the stress × time interaction, proves significant (F(1,162) = 7.64, p = 0.013 and F(1,342) = 9.05, p = 0.008, respectively). Inset, Representative fEPSPs taken at the time indicated on the graph below. Calibration: 0.5 mV, 10 ms. C, Cumulative probability distribution of the normalized fEPSPs represented as averages in B. In this and subsequent figures, each point is the average of two successive responses, and dashed lines represent 100% of baseline. Data are represented as mean ± SEM.
- Figure 2.
The facilitatory effect of acute stress on DHPG-LTD is not associated with a change in the PPR. A, Individual PPR absolute values before (baseline), during (DHPG 50 μm), and after (wash) a 10 min application of DHPG to slices from control and stressed rats (n = 8 per group). Inset, Representative fEPSPs taken at the time indicated on the graph below. Calibration: 0.5 mV, 10 ms. B, Percentage of PPR changes from baseline during (DHPG 50 μm) and after (wash) a 10 min application of DHPG to slices from control and stressed rats. The PPR increases during (*p < 0.05) but not after DHPG application in both slice groups. Data are represented as mean ± SEM.
- Figure 3.
The facilitatory effect of acute stress on DHPG-LTD is mediated by GRs. A, A 10 min application of 50 μm DHPG elicits LTD in slices from stressed but not from control rats pretreated with vehicle (Veh). Whether analyzed during the last 10 or 20 min of recordings, stress, but not the stress × time interaction, proves significant (F(1,126) = 11.72, p = 0.004 and F(1,266) = 10.09, p = 0.007, respectively). B, Cumulative probability distribution of the normalized fEPSPs represented as averages in A. C, A 10 min application of 50 μm DHPG fails to induce LTD in slices from stressed rats pretreated (30 min before stress) with RU486. D, Cumulative probability distribution of the normalized fEPSPs represented as averages in C. Data are represented as mean ± SEM.
- Figure 4.
DHPG-LTD in slices from stressed rats is mediated by mGluR1. A, The short-term inhibitory effect of a 10 min application of 50 μm DHPG in control slices is weakened either by 50 μm of the mGluR1 antagonist CPCCOEt or by 10 μm of the mGluR5 antagonist MPEP. B, Cumulative probability distribution of the normalized fEPSPs represented as averages in A. C, Treatment with 50 μm of the mGluR1 antagonist CPCCOEt weakens the short-term inhibitory effect of a 10 min application of 50 μm DHPG and blocks DHPG-LTD in slices from stressed rats. Whether analyzed during the last 10 or 20 min of recordings, stress, but not the stress × time interaction, proves significant in vehicle-pretreated (F(1,162) = 6.18, p = 0.022 and F(1,342) = 4.84, p = 0.04, respectively) and MPEP-pretreated (F(1,99) = 10.02, p = 0.01 and F(1,209) = 8.96, p = 0.013, respectively) slices but not in CPCCOEt-pretreated slices. D, Cumulative probability distribution of the normalized fEPSPs represented as averages in C. For clarity, MPEP is not presented in B and D. Veh, Vehicle. Data are represented as mean ± SEM.
Additional Files
Supplemental Data
Files in this Data Supplement:
- supplemental material - Figure legends
- supplemental material - Figure 1
- supplemental material - Figure 2
- supplemental material - Figure 3
