Abstract
Peripheral axons of dorsal root ganglion (DRG) neurons, but not their central axons in the dorsal columns, regenerate after injury. However, if the neurons are conditioned by a peripheral nerve injury into an actively growing state, the rate of peripheral axonal growth is accelerated and the injured central axons begin to regenerate. The growth-promoting effects of conditioning injuries have two components, increased axonal growth and a reduced response to inhibitory myelin cues. We have examined which transcription factors activated by peripheral axonal injury may mediate the conditioning effect by regulating expression of effectors that increase the intrinsic growth state of the neurons. Activating transcription factor 3 (ATF3) is a prime candidate because it is induced in all injured DRG neurons after peripheral, but not central, axonal damage. To investigate if ATF3 promotes regeneration, we generated transgenic mice that constitutively express this transcription factor in non-injured adult DRG neurons. The rate of peripheral nerve regeneration was enhanced in the transgenic mice to an extent comparable to that produced by a preconditioning nerve injury. The expression of some growth-associated genes, such as SPRR1A, but not others like GAP-43, was increased in the non-injured neurons. ATF3 increased DRG neurite elongation when cultured on permissive substrates but did not overcome the inhibitory effects of myelin or promote central axonal regeneration in the spinal cord in vivo. We conclude that ATF3 contributes to nerve regeneration by increasing the intrinsic growth state of injured neurons.
Introduction
Peripheral nerve regeneration involves cytoskeletal protein assembly, formation of new membrane and expression of multiple adhesion molecules and receptors at growth cones (Goodman, 1996; Heidemann, 1996; Gordon-Weeks, 2004). This is initiated by retrograde signals from the injury site (Snider et al., 2002) that, by altering transcription of multiple genes in the neuronal cell body (Costigan et al., 2002; Xiao et al., 2002), increase the intrinsic growth state of the neurons (Plunet et al., 2002; Makwana and Raivich, 2005). That transcription is required for axonal growth is shown by the prevention of neurite elongation by RNA polymerase II inhibitors (Smith and Skene, 1997; Cai et al., 2002). Several putative growth-associated genes, including GAP-43, CAP-23, SPRR1A, and Hsp27, are induced in conditions when neurons actively grow, and can, when overexpressed in neurons, increase axonal growth (Aigner et al., 1995; Strittmatter et al., 1995; Caroni et al., 1997; Bomze et al., 2001; Bonilla et al., 2002; Williams et al., 2006). The increased intrinsic growth of DRG neurons triggered by peripheral axonal injury, “conditions” the neurons to grow more extensively in culture (Hu-Tsai et al., 1994; Smith and Skene, 1997) and accelerates peripheral nerve regeneration in vivo (McQuarrie et al., 1977; Bisby and Pollock, 1983; Sjoberg and Kanje, 1990). Peripheral nerve conditioning injuries also permit DRG neuronal growth on normally nonpermissive central myelin substrates (Neumann et al., 2002; Qiu et al., 2002), and in vivo, enable the central axons of DRG neurons to grow into and beyond lesions of the dorsal columns of the spinal cord (Neumann and Woolf, 1999; Neumann et al., 2005). This indicates that peripheral axonal injuries can produce a diminished response to inhibitory myelin cues in addition to increasing the growth state of the neurons. Both of these changes are likely necessary for regeneration in the CNS. However, it is unclear whether a single transcriptional regulator or parallel independent regulatory pathways control the relevant effector genes.
Several transcription factors including c-Jun, STAT3, JunD, Sox11, CREB, and ATF3 are elevated and in some cases also activated in the DRG after peripheral injury, whereas others such as ATF2 are downregulated (Jenkins and Hunt, 1991; Leah et al., 1991; Herdegen et al., 1992; Martin-Villalba et al., 1998; Schwaiger et al., 2000; Tsujino et al., 2000; Tanabe et al., 2003; Lee et al., 2004; Jankowski et al., 2006). The failure of a central axonal injury to condition DRG neurons into an actively growing state and induce growth-associated genes (Chong et al., 1994; Smith and Skene, 1997; Mason et al., 2002; Wallquist et al., 2004) suggests that transcription factors induced only after a peripheral axonal injury are the trigger that conditions neurons to regenerate. ATF3 is highly induced in all injured DRG neurons after peripheral but not central axonal injury and is downregulated on reinnerevation of the peripheral target (Tsujino et al., 2000; Bloechlinger et al., 2004). Delivery of ATF3 to cultured adult DRG neurons, neonatal SCG neurons and PC12 cells enhances neurite outgrowth (Nakagomi et al., 2003; Pearson et al., 2003; Seijffers et al., 2006).
We now have generated transgenic mice that constitutively express ATF3 in non-injured adult DRG neurons to evaluate the specific role of this transcription factor on intrinsic growth.
Materials and Methods
Generation of ATF3 transgenic mice.
ATF3 mouse coding sequence (cds) upstream to an IRES element and the human placental alkaline phosphatase (hPLAP) gene were cloned as a 2.8 kb fragment into the XhoI site of the Thy-1.2 expression cassette (Caroni, 1997). The transgene encompassing the Thy-1.2-regulating elements, ATF3 cds, IRES element, and the hPLAP reporter gene was microinjected into fertilized B6C3F1 mouse oocytes, for the generation of transgenic mice. Three sets of primers verified that the whole transgene had integrated into the mouse genome. Primer 1, 5′-GAG ATG TCA GTC ACC AAG TCT G-3′, and primer 2, 5′-GGA TAA AGA GGT TCC TCT CGT C-3′, amplify a fragment in the ATF3 cds. Another set is comprised of one primer situated on the ATF3 cds and the other on the Thy-1.2 promoter: primer 3, 5′-AGG ACC TTA GGC AGT GTC ACT C-3′, and primer 4, 5′-CTT CCT TGA CAA AGG GTG TCA G-3′. A third is specific for the hPLAP gene: primer 5, 5′-GAC AAA CAT GTG CCA GAC AGT G-3′, and primer 6, 5′-AAC ATG TAC TTT CGG CCT CCA C-3′ (see Fig. 1). Mice with the transgene in their genome were bred on to a C57Bl/6 background.
Surgery.
Procedures were performed in accordance with the Massachusetts General Hospital Animal research regulations. Experiments were performed under isoflurane anesthesia (Baxter, Deerfield, IL) in adult mice 8 weeks and older. Sciatic nerve transection (SNT) was performed at mid-thigh level; the exposed nerve was ligated, and the nerve was transected distally to the ligation. For crush injuries the sciatic nerve was crushed with smooth forceps for 15 s at the proximal thigh level, and the injury site was marked with a 10-0 epineural suture. Dorsal column lesion (DC) was performed by a hemi-laminectomy at T10, transecting the dorsal column on both sides with ophthalmic microscissors.
Primary adult DRG neuron culture.
DRGs were digested in collagenase (5 mg/ml)/dispase (1 mg/ml) (Roche, Indianapolis, IN), dissociated in 0.25% trypsin (Cellgro, Herndon, VA) and mechanically triturated through a polished Pasteur pipette to a single-cell suspension. Cells were purified on 10% BSA in PBS solution (Sigma, St. Louis, MO) to enrich the cultures for neurons (>90%). Then the cells were plated in a tissue culture eight-well chamber slide dish (Nalge Nunc, Naperville, IL) coated with poly-d-lysine and laminin (Sigma) or poly-d-lysine and 0.75 mg of myelin per well and cultured in Neurobasal medium (Invitrogen, Carlsbad, CA) supplemented with B27 supplement, penicillin, streptomycin, 1 mm l-glutamine, 50 ng/ml NGF, 2 ng/ml GDNF, and 10 mm AraC at 37°C.
Alkaline phosphatase activity assay.
Whole-mount alkaline phosphatase (hPLAP) activity was assayed in freshly dissected mouse DRGs, fixed in 0.5% glutaraldehyde, washed in PBS, incubated in prewarmed PBS at 65°C for 30 min, washed in 100 mm Tris-HCl, pH 9.5, 100 mm NaCl, 50 mm MgCl2, 0.1% Tween 20, and 0.48 g/L levamisole, and incubated overnight with BM purple AP substrate (Roche) in the dark.
Immunostaining.
Dissociated DRG neurons or 7-μm-thick DRG cryosections were fixed in 4% paraformaldehyde, washed in PBS, blocked in 2% goat serum/0.3% Triton X-100 in PBS, and processed with 1:500 anti-ATF3 (rabbit polyclonal) antibody (Santa Cruz Biotechnology, Santa Cruz, CA), 1:50 anti-c-Jun (rabbit monoclonal) antibody (Cell Signaling Technology), 1:800 anti-β-tubulin III (mouse monoclonal) antibody (Sigma), 1:1000 anti-GAP-43 (rabbit polyclonal) (Chemicon, Temecula, CA), 1:1000 anti-SPRR1A (rabbit polyclonal) (Abcam, Cambridge, MA), followed by 1:200 Cy3-anti-rabbit (goat polyclonal) antibody (Jackson ImmunoResearch, West Grove, PA).
Western blot analysis.
Protein was extracted from adult L5 and L6 DRG by using RIPA buffer and concentration determined by using the BCA kit (Pierce, Rockford, IL). Then 20 μg of protein was resolved on a 4–15% SDS-PAGE gradient gel (Bio-Rad, Hercules, CA). The gel was transferred to 0.45 μm PVDF membrane (Millipore, Bedford, MA), blocked in 5% skim milk, 0.05% Tween 20 in PBS, and incubated with 1:500 anti-ATF3 (rabbit polyclonal) antibody (Santa Cruz Biotechnology). The membrane was stripped and reblotted with 1:1000 anti-c-Jun (rabbit monoclonal), 1:400 anti-β-tubulin III (mouse monoclonal) (Sigma), 1:1000 anti-GAP-43 (rabbit polyclonal) (Chemicon), followed by 1:5000 horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (Amersham, Arlington Heights, IL) and visualized by using enhanced chemiluminescence detection (PerkinElmer Life Sciences, Boston, MA).
Neurite outgrowth assay.
Neurite outgrowth was assayed in DRG neurons cultured on laminin or myelin for either 17 or 45 h. The cells were fixed with 4% paraformaldehyde and immunostained with anti-β-tubulin III. Neurons with any process were defined as bearing neurites. The percentage of neurons bearing neurites was calculated and neurite length measured with the NIH ImageJ software. The longest process for 100 individual neurons in each replicate was measured and averaged. A neurite length frequency histogram was used to display the neurite length distribution.
Measurement of nerve regeneration.
The nerve pinch test was used to determine the rate of in vivo peripheral nerve regeneration (Sjoberg and Kanje, 1990; Mills et al., 2005). This technique is used to evaluate enhanced regeneration, and is performed between 2 and 4 d after injury. At 2 d after sciatic crush, anesthesia was induced with 2.5% isoflurane, and the left sciatic nerve was exposed. Animals were brought to a light plane of anesthesia (1% isoflurane). Starting distally, a series of pinches using fine smooth forceps were delivered to the sciatic nerve, moving proximally toward the injury site. We determined the rate of regeneration by measuring the distance from the injury site, which was marked with an epineural suture under a dissecting microscope, to the most distal point on the nerve that produced a reflex withdrawal response when pinched. GAP-43 immunostaining was used to characterize regeneration histologically, because GAP-43 expression is highly induced in regenerating fibers in the injured sciatic nerve 2 d after injury. Sciatic nerves were sectioned serially at 10 μm thick, and the mean number of GAP-43-immunoreactive fibers per section was determined in three sections (30 μm apart) per animal, 1.5 mm beyond the injury site. The mean number of fibers per section per animal was determined for three animals in each treatment group.
Central regeneration was assayed 4 weeks after DC injury by injection of 2 ml of 1% B-HRP (List Biologic, Campbell, CA) into the sciatic nerve. At 4 d later horizontal sections (10 mm thick) through the upper lumbar and thoracic spinal cord were processed for peroxidase activity (Neumann and Woolf, 1999). Labeled fibers were traced by using a camera lucida and the length of fibers regenerating from the center of the lesion site and rostral measured. All measurements were blinded to the mouse genotype/treatment.
Real-time quantitative PCR.
The L5 and L6 DRGs of four to five mice for each group were pooled, RNA was extracted via Trizol (Invitrogen) and purified with the RNeasy kit (Qiagen Sciences, Germantown, MD), and genomic DNA eliminated with DNaseI digestion (Qiagen, Hilden, Germany). cDNA was generated, using the first strand synthesis kit (Invitrogen). Mouse-specific primers for ATF3, c-Jun, GAP-43, CAP-23, SPRR1A, α7 integrin, Hsp27, STAT3, and GAPDH (SuperArray, Fredrick, MD) were amplified by polymerase chain reaction in the presence of SYBR green (SuperArray). The levels of the target gene were compared with GAPDH, and fold difference was calculated compared with a littermate control.
Results
Creation of ATF3 transgenic mice
Mouse ATF3 cDNA and the hPLAP reporter were cloned as a bicistronic expression cassette into the thy1.2 expression vector. The thy1.2 regulatory elements enable postnatal neuronal-specific expression with a sustained expression through adulthood that is not affected by nerve injury (Caroni, 1997). The transgenes and the thy1.2 regulatory elements bordered by NotI and PvuI were used for microinjection (Fig. 1). The resulting progeny were genotyped, using primer sets spanning ATF3, hPLAP, and the thy1.2 promoter (Fig. 1 A). Seven ATF3 mouse lines were produced and tested for ATF3 transgene protein expression in DRG neurons. As a first screen a whole-mount alkaline phosphatase assay was performed on DRGs from the ATF3 mouse lines to identify hPLAP reporter activity. The hPLAP reporter expression was identified clearly in the DRG from ATF3 transgenic mice, with no expression in control wt mice (Fig. 1 B,C). Next the amount of ATF3 protein its distribution in the DRG of non-injured ATF3 transgenic mice lines was determined by immunostaining and Western blot analysis (Fig. 2). ATF3 protein levels were compared with control wt non-injured and injured DRG neurons (3 d after a sciatic nerve transection) and with littermate non-injured controls. Almost no ATF3 expression was detected in non-injured wt DRG and littermate controls, and high expression was detected in wt DRG 3 d after peripheral axotomy (Fig. 2 A–C,K, lanes 1, 2, 10). The amount of ATF3 protein expressed differed between the different ATF3 transgenic lines tg1–tg7 (Fig. 2 D–J,K, lanes 3–9). ATF3 in the transgenic mouse lines was detected in both the nucleus and cytoplasm of DRG neurons, with no expression in non-neuronal cells. All ATF3 transgenic mouse lines were viable and fertile and showed no obvious behavioral abnormalities. Two of the ATF3 mouse lines that expressed levels of ATF3 most comparable to the ATF3 levels induced in peripheral nerve-injured wt DRG neurons were used for additional analysis.
Generation of ATF3 transgenic mice. A , ATF3 and a hPLAP reporter were cloned bicistronicly into the XhoI site of the thy1.2 expression cassette. The transgenes and regulatory elements were excised with NotI/PvuI and used to generate ATF3 transgenic mice. Primer sets 1 and 2 (blue bands), 3 and 4 (red bands), and 5 and 6 (pink bands) were used for genotyping. B , C , The wt ( B ) and ATF3 ( C ) transgenic DRG whole mounts that were stained for alkaline phosphatase activity show expression of the reporter in the transgenic mouse DRG.
Constitutive ATF3 expression in L5 DRGs of non-injured ATF3 transgenic mice. A–J , Immunostaining for ATF3 in L5 DRGs of wt ( A ), wt 3 d post-SNT ( B ), a littermate control ( C ), and seven different non-injured ATF3 transgenic mouse lines, tg1–tg7 ( D–J ). Scale bars, 100 μm. K , Western blot analysis for ATF3 in L5 and L6 DRGs. Lane 1, wt; lane 2, 3 d post-SNT; lanes 3–9, non-injured tg1–tg7; lane 10, littermate control.
Peripheral nerve regeneration is enhanced in ATF3 transgenic mice
The rate of peripheral nerve regeneration 2 d after a sciatic nerve crush was measured via the nerve pinch test. An early time point of 2 d after injury was used to evaluate whether ATF3 constitutively expressed before the injury can prime the neurons for growth. The pinch test determines the extent of axonal regrowth after a crush injury by measuring the distance between the point of injury and the most distal point on the nerve that produces a reflex withdrawal response when pinched with forceps (Sjoberg and Kanje, 1990; Mills et al., 2005). The test only measures growth of sensory axons because pinch of motor axons does not elicit withdrawal reflexes.
The rate of regeneration was enhanced in two separate ATF3 transgenic mouse lines compared with littermate controls (Fig. 3 A). Enhanced regeneration was also found in control wt mice preconditioned by a crush injury 3 d before a second crush injury. The increased early regeneration rate in the two ATF3 transgenic mice lines was similar to that produced by the preconditioning lesion (Fig. 3 A). The extent of sciatic nerve regeneration was additionally analyzed, using GAP-43 as a marker for regenerating axons. The number of GAP-43-positive fibers 1.5 mm distal to the crush site, measured 2 d after the injury, doubled in the ATF3 transgenic mice compared with littermate controls (Fig. 3 B–D). The acceleration in nerve regeneration in the ATF3 transgenic mice as revealed both by the pinch test and GAP-43 reactivity is illustrated in Figure 3 E. GAP-43 expression is not enhanced in the transgenic mice (see below) enabling its use as an axonal marker. Although denervated Schwann cells do express GAP-43 (Curtis et al., 1992), the level is much lower than that in regenerating axons, and under the staining conditions used in these experiments, was not detected. Enhanced peripheral nerve regeneration was found in two independent ATF3 transgenic mouse lines indicating that the growth effect was not a result of the site of DNA integration, but of the ATF3 expression. ATF3 therefore increases peripheral axonal growth in vivo to an extent equivalent to the increase produced by a conditioning lesion implying that uninjured DRG neurons in the ATF3 transgenic mice are primed for growth before axonal injury.
ATF3 enhances peripheral nerve regeneration. A , Sciatic nerve regrowth assayed by the nerve pinch test 48 h after crush of the sciatic nerve increases in two separate ATF3 transgenic lines (tg1 and tg4; black and blue bars) compared with their littermate controls (striped bars). Preconditioned wt (sciatic conditioning crush 3 d before the second test crush) were used as positive controls for enhanced growth (gray bars); n = 6–9 per group. *p < 0.01; **p < 0.001; Student's t test. B , GAP-43 expression in regenerating fibers 48 h after nerve crush in two ATF3 transgenic mice lines, tg1 and tg4, compared with littermate controls. *p < 0.01; **p < 0.001; Student's t test. C , D , Representative nerve sections stained for GAP-43 in the littermate control and ATF3 transgenic line, respectively. Scale bars, 200 μm. E , Graphic representation of the extent of regrowth detected by nerve pinch test and GAP-43-immunoreactive axons in ATF3 transgenic (bottom panel) and littermate control (top panel). 0 mm, Crush point; black thin lines, GAP-43-positive regenerating fibers; dashed arrow, direction of fiber growth from the point of crush toward the peripheral target; filled arrow, average distance point a flinch response appears when the nerve is pinched 2 d after injury. The dotted line between 1.5 and 2 mm represents the area of GAP-43 fiber quantification.
Failure of regeneration after dorsal column injury
To test if ATF3 enhances regeneration in the CNS, we performed a dorsal column injury, injuring the axons of medium and large DRG neurons that ascend up the dorsal columns in the spinal cord to the gracile nucleus. B-HRP was injected into the sciatic nerve to trace dorsal column axons emanating from the nerve 4 weeks after the spinal cord injury. In wt mice no regeneration occurred in the dorsal column and similarly no regeneration developed in ATF3 transgenic mice (Fig. 4 A,B,D). In wt mice in which a conditioning sciatic nerve transection was performed 4 d before the dorsal column injury, growth occurred into the lesion site in the spinal cord with sprouting caudal to the injury, many axons extending into the lesions site and a few axons extending rostrally (Fig. 4 C,D). None of these three manifestations of enhanced growth were observed in the non-preinjured wt or ATF3 transgenic mice. Although constitutive ATF3 expression promotes peripheral regeneration it does not fully mimic the conditioning effect that enables some regeneration in the CNS.
Constitutive ATF3 expression does not promote regeneration after dorsal column injury. A–C , Representative spinal cord sections stained for B-HRP show the extent of growth 4 weeks after dorsal column injury in wt mice ( A ), ATF3 transgenic mice ( B ), and preconditioned (with a sciatic nerve transection) wt mice ( C ). D , Preconditioning, but not constitutive, ATF3 expression increased growth into the lesion site.
ATF3 enhances neurite outgrowth of adult DRG neurons on a permissive substrate but fails to overcome myelin inhibition
To determine if some aspects of the spinal cord environment may inhibit axonal growth in the ATF3 transgenic mice after a dorsal column injury as it does in intact wt but not preconditioned wt mice, we cultured adult DRG neurons on a permissive laminin or on an inhibitory central myelin substrate. Adult DRG cultures were prepared from two ATF3 transgenic mouse lines and their control littermates. The neurons were plated and 17 h later, stained for β-tubulin III. When grown on the laminin substrate the increase in the proportion of neurons bearing neurites in ATF3 transgenic mice lines compared with littermate controls was small (5–7%) (Fig. 5 A). However, neurite length increased substantially (Fig. 5 B–D). The enhancement in neurite outgrowth was comparable to that found by delivering ATF3 with HSV based amplicon vectors to cultured adult DRG neurons (Seijffers et al., 2006). In contrast, no significant difference between ATF3 transgenics and littermate control DRG neurons was identified when cultured on myelin for 17 h. The percentage of DRG neurons bearing neurites (6.7 ± 0.9 tg vs 6.6 ± 0.5% control littermate) and average neurite length (94.5 ± 4.3 μm tg vs 90.5 ± 7.4 μm control littermates) were not different, nor was the neurite length frequency distribution (Fig. 5 E). In addition, no significant difference between ATF3 transgenic mice and littermate controls was observed after culturing the DRG neurons for 45 h on myelin in either the percentage of neurons bearing neurites (39.8 ± 2.7 and 34.8 ± 1.8%, respectively) or average neurite length (211 ± 16.7 μm for tg compared with 167.02 ± 8.9 μm in the littermates). The neurite length frequency distribution also did not differ significantly, although at 45 h the growth was greater than at 17 h (F). Constitutive ATF3 expression increases neuronal growth on a permissive environment but, unlike peripheral nerve-conditioning lesions (Neumann et al., 2002; Qiu et al., 2002), does not overcome the inhibition produced by CNS myelin in culture. This may at least in part explain the failure of central regeneration after dorsal column injury in the transgenic mice.
ATF3 enhances neurite outgrowth in a permissive environment but does not overcome myelin inhibition. A , B , The percentage of neurons extending neurites ( A ) and the average neurite length measured after 17 h in culture ( B ) increased in two independent ATF3 transgenic mouse lines, tg1 (black bars) and tg4 (blue bars), compared with their littermate controls (striped bars) when cultured on laminin. Mean ± SEM; n = 6. # p < 0.05; *p < 0.01; **p < 0.001; ***p < 0.0001; Student's t test. C , D , Representative images immunostained for β-tubulin III in ATF3 transgenic and littermate control, respectively. Scale bars, 100 μm. E , F , Neurite length frequency histograms of DRG neurons cultured on myelin for 17 h ( E ) and 45 h ( F ) show no significant difference between transgenic mice and littermate controls. Mean ± SEM; n = 6; p > 0.05; Student's t test.
Change in growth-associated gene expression in ATF3 transgenic mice
Quantitative real-time PCR was performed to test for ATF3-dependent changes in the transcription of putative growth associated genes. Transcript levels for ATF3, Hsp27, SPRR1A, c-Jun, CAP-23, GAP-43, α7 integrin and STAT3 were measured and normalized to GAPDH (Fig. 6) in L5 and L6 DRGs of non-injured ATF3 transgenic, littermate control and wt mice 3 d after a sciatic nerve transection. ATF3 mRNA levels in the ATF3 transgenic mice were similar to that found after axotomy in wt mice (Fig. 6 A). Hsp27 is the only ATF3 regulated gene so far described in neurons (Nakagomi et al., 2003) and its levels increased in the ATF3 transgenic mice (Fig. 6 B). SPRR1A (8.48-fold) and c-Jun (1.93-fold) levels also elevated in the DRGs of non-injured ATF3 transgenic mice, but CAP-23, GAP-43, α7 integrin, and STAT3 levels did not change despite their all being induced by peripheral nerve injury (Fig. 6 C–H). Immunostaining and Western blot analysis confirmed that c-Jun and SPRR1A protein were increased in intact ATF3 transgenic mice DRGs, with no change in GAP-43 (Fig. 7). ATF3 expression in non-injured DRG neurons therefore is adequate to trigger an upregulation of some, but not all, peripheral injury-induced putative growth-associated genes.
ATF3-dependent transcriptional changes of growth-associated genes. Fold change in the mRNA levels of ATF3, Hsp27, SPRR1A, c-Jun, CAP-23, GAP-43, α7 integrin, and STAT3 ( A–H ) in L5 and L6 DRGs of non-injured ATF3 transgenic mice, wt 3 d after sciatic nerve transection (Ax), and non-injured littermate control was measured by quantitative real-time PCR. Mean ± SD; n = 6. # p < 0.05; *p < 0.01; **p < 0.001; ***p < 0.0001; Student's t test.
Changes in levels of growth-associated proteins in non-injured ATF3 transgenic and sciatic nerve-injured wt mice. A , Western blots for ATF3, c-Jun, and GAP-43 in L5 and L6 DRGs of naive (lane 1), 3 d post-SNT (lane 2), littermate (lane 3), and three ATF3 transgenic mice lines, tg4, tg1, and tg2 (lanes 4–6). B–M , L5 DRGs of naive, 3 d post-SNT (Ax), and non-injured ATF3 transgenic were immunostained for ATF3 ( B–D ), c-Jun ( E–G ), GAP-43 ( H–J ), and SPRR1A ( K–M ). The inset in M shows nerve fibers that are positive for SPRR1A in the ATF3 transgenic mice. Scale bars, 20 μm.
Discussion
Peripheral nerve injury, unlike axonal damage within the central nervous system, triggers injured neurons into an actively growing state, an essential prerequisite for successful regeneration. Once primary sensory neurons are primed by peripheral axonal injury, they grow more rapidly in response to a subsequent lesion, in what has become known as the conditioning effect (Richardson and Issa, 1984; Sjoberg and Kanje, 1990; Oudega et al., 1994). In addition to increasing growth on permissive environments such as laminin or Schwann cells, the peripheral conditioning lesion also overcomes the effects of myelin growth inhibitory cues. Injury to the peripheral axon of a primary sensory neuron enables, for example, its central axon in the dorsal columns to regenerate into and beyond a lesion site (Neumann and Woolf, 1999; Neumann et al., 2005). The inhibition of growth evoked by myelin of oligodendrocyte origin is attributable to several membrane-associated proteins that include Nogo, myelin-associated glycoprotein, and OMgp (Mukhopadhyay et al., 1994; Chen et al., 2000; GrandPre et al., 2000; Domeniconi et al., 2002; Liu et al., 2002; Wang et al., 2002). Other inhibitory cues include extracellular matrix molecules such as chondroitin sulfates (Silver and Miller, 2004). The reduction in the response of neurons to these inhibitory cues after peripheral nerve injury could be attributable to a modification or downregulation in the receptors that comprise the Nogo receptor complex. Expression of p75 in DRG neurons is downregulated after peripheral injury (Krekoski et al., 1996; Zhou et al., 1996) and it is possible that levels of NgR1, Troy or lingo are also decreased (GrandPre et al., 2002; Mi et al., 2005; Park et al., 2005; Shao et al., 2005). Changes in the expression or modification of downstream signal transduction paths or other effectors that lead to growth cone collapse may also contribute (Lehmann et al., 1999; Dergham et al., 2002; Sivasankaran et al., 2004; Spencer and Filbin, 2004; Domeniconi et al., 2005; Koprivica et al., 2005). The conditioning effect contributes therefore, both to an enhancement of axonal growth and a diminished response to myelin inhibitory signals.
Peripheral injury is accompanied by altered transcription of >1000 genes in the DRG (Costigan et al., 2002; Xiao et al., 2002). These include induction or increased expression of several candidate growth-associated genes with established or postulated roles in the formation or elongation of neurites and that may constitute the intrinsic, transcriptionally regulated molecular machinery necessary for axonal growth (Snider et al., 2002; Makwana and Raivich, 2005). We hypothesized that ATF3 contributes to nerve regeneration by coordinating expression of growth-associated genes, both because it is the only known transcription factor induced in all injured DRG neurons after a peripheral but not central axonal injury (Tsujino et al., 2000; Bloechlinger et al., 2004), and because it enhances neurite outgrowth in culture (Nakagomi et al., 2003; Pearson et al., 2003; Seijffers et al., 2006). We now show that ATF3 does indeed enhance the rate of regeneration in the PNS to an extent similar to wt mice preconditioned by a nerve injury. We conclude that ATF3 may be a major driver of the peripheral nerve regeneration-promoting effects of conditioning lesions. However, constitutive ATF3 expression in non-injured adult DRG neurons does not, unlike a peripheral nerve injury, overcome the inhibitory effects of central myelin in vivo or in vitro. This indicates that constitutive ATF3 expression is not sufficient on its own to fully mimic the conditioning effects to trigger central axonal regeneration. These observations do not however exclude the possibility that ATF3 still contributes to regeneration in the CNS. ATF3 may, for example, contribute to the reduced response to inhibitory myelin cues after peripheral nerve injury but only when acting together with other transcription factors. ATF3 can form functional heterodimers with leucine zipper transcription factors such as c-Jun and CREB and both are upregulated in DRG neurons by nerve injury. ATF3 may also interact with nonleucine zipper transcription factors upregulated after peripheral injury, such as, STAT3. This could be via coactivators or by synergizing with transcription factors that bind to adjacent DNA binding sites. Furthermore, peripheral nerve injury may produce post-translational modifications in ATF3 that are required for its full function, and these may not be present in non-injured neurons in the transgenic mice. Studies that force ATF3 expression in DRG neurons in concert with other transcription factors, as well as experiments that involve both preconditioning injuries and ATF3 knockdown are required to evaluate the extent to which this transcription factor contributes to all of the different aspects of the conditioning paradigm; induction of growth associated genes, increased axonal growth, and decreased response to inhibitory cues.
Constitutive ATF3 expression in non-injured adult DRG neurons did not produce all of the changes in expression of a panel of growth-associated genes that are induced by peripheral nerve injury. In the transgenic DRGs we found an increase in c-Jun, Hsp27, and SPRR1A but not of GAP-43, CAP-23, α7 integrin, or STAT3. Nevertheless, the ATF3-regulated expression of these and perhaps other genes in non-injured neurons are sufficient to prime the neurons for enhanced peripheral axonal regeneration. However, as mentioned above, additional transcription factors and post-translational changes induced after nerve injury may interact and/or act in concert with ATF3 to produce the complete set of ATF3 dependent transcriptional changes. Constitutive ATF3 expression in the transgenic mice although sufficient to enhance axonal growth may not fully reflect the entire role of this transcription factor in nerve regeneration after injury.
ATF3 functions both as an activator and a repressor to control expression of a variety of genes (Hai et al., 1999; Hai and Hartman, 2001). In neurons, the only identified ATF3 target so far is the heat shock protein Hsp27, a peripheral nerve injury-induced intrinsic neuronal survival factor (Benn et al., 2002). ATF3 binds directly to the Hsp27 promoter and activates its expression in PC12 cells (Nakagomi et al., 2003). ATF3 transgenic mice show enhanced Hsp27 expression, confirming in vivo that Hsp27 is an ATF3 target gene. In addition to its role as a survival factor, Hsp27 enhances neurite outgrowth in cultured adult DRG neurons (Williams et al., 2006), possibly as a result of interactions with the cytoskeleton, and may therefore contribute to the enhanced peripheral nerve regeneration in the transgenic mice. The growth-associated gene SPRR1A, which is massively induced in all injured DRG neurons, is also elevated in non-injured neurons in ATF3 transgenic mice, although to a more limited extent. SPRR1A promotes axonal outgrowth by interacting with actin structures (Bonilla et al., 2002). ATF3 may regulate expression of SPRR1A either by binding directly to the promoter or affecting other transcription factors. The SPRR1A promoter contains functional AP-1 binding sites (Sark et al., 1998; Pradervand et al., 2004). AP-1 sites are recognized by homodimers of Jun or hetreodimers of Jun/Fos and ATF/CREB bZIP transcription factors (Hai and Curran, 1991). Because the levels of SPRR1A in injured DRGs are higher than in non-injured DRG in the ATF3 transgenic mice, other downstream signals in addition to ATF3 are likely to govern SPRR1A expression. C/EBP β, for example, is induced after peripheral nerve injury and a functional C/EBP β binding site is located on the SPRR1A promoter (Pradervand et al., 2004; Nadeau et al., 2005).
The upregulation of c-Jun in the DRG of ATF3 transgenic mice implies that ATF3 may control c-Jun expression. A neuronal specific knockout of c-Jun decreases the rate of regeneration of injured facial motor neuron axons (Raivich et al., 2004), and JNK inhibitors reduce neurite outgrowth (Lindwall et al., 2004). Both ATF3 and c-Jun promoters contain functional AP-1 sites (Morooka et al., 1995; Cai et al., 2000) and c-Jun and ATF3 may therefore regulate each other's expression. ATF3 synergizes with c-Jun to enhance neurite outgrowth (Pearson et al., 2003; Lindwall et al., 2004). The growth-associated gene α7 integrin (Werner et al., 2000) is downregulated in the facial nucleus of c-Jun conditional knockout mice (Raivich et al., 2004), but its expression is unchanged in ATF3 transgenic mice. This implies that, although c-Jun is upregulated by ATF3 transgenic expression, this is not enough by itself to alter expression of α7 integrin, suggesting that its expression depends on additional signals in DRG neurons.
When cAMP is elevated in the DRG, neurites overcome myelin inhibition and axons grow in the spinal cord after a dorsal column lesion (Neumann et al., 2002; Qiu et al., 2002). cAMP signals through PKA to initiate CREB activation that then upregulates Arginase I with subsequent synthesis of polyamines. Polyamines probably act by affecting cytoskeleton assembly (Cai et al., 2002; Gao et al., 2004). PKA also inhibits RhoA activation, therefore preventing growth cone collapse. In addition, cAMP elevates IL-6, which signals via the JAK/STAT pathway to overcome myelin inhibition (Cao et al., 2006). IL-6 signals through Janus kinases to activate STAT3, and both IL-6 knock-out and inhibition of STAT3 diminish the axonal growth evoked by a preconditioning injury (Cafferty et al., 2004; Qiu et al., 2005). An increase in STAT3 mRNA levels is detected in injured motor neurons (Schwaiger et al., 2000), but more striking is the change in its phosphorylation state after injury (Schwaiger et al., 2000; Qiu et al., 2005). Although we found a modest increase in STAT3 mRNA level in the DRG after nerve injury, no change was found in the ATF3 transgenics. Similarly, no enhancement in STAT3 phosphorylation was identified in non-injured ATF3 transgenic mice (data not shown). In addition, GAP-43 mRNA levels were unchanged in the non-injured transgenic mouse DRG, implying that this prototypic growth-associated gene is not induced by ATF3 expression alone. Constitutive ATF3 expression appears to be functionally distinct from the effects of cAMP because it does not modify growth on myelin. Nevertheless, it is possible that transcription factors downstream of cAMP signaling could interact or act concurrently with ATF3 to enhance axonal growth and overcome inhibitory cues in the CNS.
Constitutive ATF3 expression drives altered expression of a set of genes in sensory neurons that are sufficient to enhance neurite elongation in vitro and the rate of peripheral nerve regeneration in vivo. Although ATF3 forced expression increases axonal growth in the PNS, it does not overcome myelin inhibition in culture or drive regeneration in the CNS. It is therefore likely that additional transcription factors operating either alone or in concert with ATF3 act to reduce the response to inhibitory cues. However, ATF3 even when expressed in non-injured neurons without additional signals, seems to be a major determinant of the intrinsic growth state of the neurons.
Footnotes
-
This work was supported by the Muscular Dystrophy Association, National Institutes of Health (NS039518), and National Institute of Neurological Disorders and Stroke (NS45459 to C.D.M.). We are grateful to Dr. Pico Caroni for providing the thy1.2 expression cassette and to Dr. Constance Cepko for providing the hPLAP gene. We thank Dr. Lin Wu, Bagayev A. Igor, and Jaquelyne L. Bitler for technical assistance.
- Correspondence should be addressed to Clifford J. Woolf at the above address. woolf.clifford{at}mgh.harvard.edu
