Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Journal of Neuroscience
  • Log in
  • My Cart
Journal of Neuroscience

Advanced Search

Submit a Manuscript
  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE
PreviousNext
Toolbox

Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs

Shiaoching Gong, Martin Doughty, Carroll R. Harbaugh, Alexander Cummins, Mary E. Hatten, Nathaniel Heintz and Charles R. Gerfen
Journal of Neuroscience 12 September 2007, 27 (37) 9817-9823; DOI: https://doi.org/10.1523/JNEUROSCI.2707-07.2007
Shiaoching Gong
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Martin Doughty
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carroll R. Harbaugh
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alexander Cummins
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mary E. Hatten
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nathaniel Heintz
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Charles R. Gerfen
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Additional Files
  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    Cre expression directed to dopaminergic (A), serotonergic (B), and cholinergic (C) neurons in BAC-Cre transgenic mouse lines. A, Dopaminergic neurons are labeled in a Th BAC-Cre transgenic line with Cre expression localized in neurons in the midbrain substantia nigra pars compacta (Th BAC-CRE; green), which colocalize with Th-IR. There is a nearly complete overlap of TH Cre-driven EGFP labeling and Th-IR (insets). B, In a serotonin transporter (Slca4) BAC-Cre transgenic line, Cre is directed to serotonergic neurons in the dorsal raphe nucleus in the dorsal midbrain, which coexpress serotonin-synthesizing enzyme, tryptophan hydroxylase-IR. Insets, There is a nearly complete overlap of Slca4 Cre-driven EGFP labeling and tryptophan hydroxylase-IR in neurons in the dorsal raphe. C, In a Chat BAC-Cre transgenic line, labeled neurons in the basal forebrain coexpress Chat immunoreactivity (ChAT-IR).

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    Cre expression in forebrain circuits, with labeling of specific neuronal projection systems in the cerebral cortex (A, Etv1; B, Ntsr1) and striatum (C, Drd2; D, Drd1a). Expression is produced in layer 5 corticostriatal neurons in an Etv1 BAC-Cre line (A, Etv1). Cre is expressed in layer 6 corticothalamic neurons in an ntsr1 BAC-Cre line (B, Ntsr1). Clearly labeled are the projection axons of these neurons, which terminate in the dorsal thalamic nuclei. In the striatum, the majority of neurons are medium spiny projection neurons, which are evenly divided into striatopallidal (indirect pathway) and striatonigral (direct pathway) neurons, which selectively express the drd2 and drd1a dopamine receptors, respectively. Cre expression produced in Drd2 BAC-Cre lines (C, Drd2) is directed to striatopallidal neurons. In this line, labeled neurons in the striatum extend axons that terminate in the globus pallidus external segment (GPe). In contrast, expression produced in Drd1a BAC-Cre lines (D, Drd1a) is directed to striatonigral neurons, which have axons that extend through the globus pallidus to terminate in the internal segment of the globus pallidus (GPi) and substantia nigra (not shown).

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    A, A Pcp2 BAC-Cre transgenic line produces Cre expression that is restricted to the cerebellum localized to Purkinje cell neurons as seen in a sagittal brain section. B, A Cmtm5 BAC-Cre transgenic line produces Cre expression in oligodendrocyte cells throughout the brain. Bi, Labeled cells in a forebrain area including the cerebral cortex, underlying white matter, and striatum are seen to have the morphology of oligodendrocytes. C, Inducible Cre expression occurs in a serotonin transporter (Slca4) BAC-ER-Cre transgenic line. Mice treated with saline show no Cre expression, whereas a single treatment with tamoxifen (6 mg/kg, i.p.) produces Cre expression in serotonin neurons in the dorsal raphe.

Tables

  • Figures
  • Additional Files
    • View popup
    Table 1.

    List of BAC-Cre lines described in this study

    Gene [BAC-Cre transgenic line(s)]BAC cloneCre expression pattern (adult/developmental/partial)
    Choline acetyl transferase (Chat)RP23-246B12
        GM24/GM60Adult
        GM53Partial: motor nuclei only
    Tyrosine hydroxylase (Th)RP23-350E13
        FI12/FI172Adult plus developmental pattern
    Serotonin transporter (Slca4)RP24-335M24
        ET33/ET35Adult
        ET124Adult plus developmental pattern
    Noradrenalin transporter (Slc6a2)RP23-109O23
        FV317/FV319Adult
    ETS domain transcription factor (Etv1)RP23-250K4
        GM225Neocortex layer 5
    Neurotensin receptor 1 (Ntsr1)RP23-314D14
        GN220Neocortex layer 6
    D2 dopamine receptor (Drd2)RP23-161H15
        ER44Adult
        ER43Partial: striatopallidal neurons
    D1 dopamine receptor (Drd1a)RP23-47M2
        EY262/FK161/FK150Adult
    Purkinje cell protein (Pcp2)RP24-186D18
        GN135Adult
    Oligodendrocyte protein (Cmtm5)RP24-317F19
        GM160Adult

Additional Files

  • Figures
  • Tables
  • Supplemental Data

    Files in this Data Supplement:

    • supplemental material - Supplemental Material
    • supplemental material - Supplemental Figure 1
    • supplemental material - Supplemental Figure 2
    • supplemental material - Supplemental Figure 3
    • supplemental material - Supplemental Figure 4
    • supplemental material - Supplemental Figure 5
    • supplemental material - Supplemental Figure 6
    • supplemental material - Supplemental Figure 7
Back to top

In this issue

The Journal of Neuroscience: 27 (37)
Journal of Neuroscience
Vol. 27, Issue 37
12 Sep 2007
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
Email

Thank you for sharing this Journal of Neuroscience article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs
(Your Name) has forwarded a page to you from Journal of Neuroscience
(Your Name) thought you would be interested in this article in Journal of Neuroscience.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs
Shiaoching Gong, Martin Doughty, Carroll R. Harbaugh, Alexander Cummins, Mary E. Hatten, Nathaniel Heintz, Charles R. Gerfen
Journal of Neuroscience 12 September 2007, 27 (37) 9817-9823; DOI: 10.1523/JNEUROSCI.2707-07.2007

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Request Permissions
Share
Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs
Shiaoching Gong, Martin Doughty, Carroll R. Harbaugh, Alexander Cummins, Mary E. Hatten, Nathaniel Heintz, Charles R. Gerfen
Journal of Neuroscience 12 September 2007, 27 (37) 9817-9823; DOI: 10.1523/JNEUROSCI.2707-07.2007
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Discussion
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

  • Monosynaptic Circuit Tracing with Glycoprotein-Deleted Rabies Viruses
  • Granger Causality Analysis in Neuroscience and Neuroimaging
  • Investigating Axonal Guidance with Microdevice-Based Approaches
Show more Toolbox
  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Issue Archive
  • Collections

Information

  • For Authors
  • For Advertisers
  • For the Media
  • For Subscribers

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
(JNeurosci logo)
(SfN logo)

Copyright © 2023 by the Society for Neuroscience.
JNeurosci Online ISSN: 1529-2401

The ideas and opinions expressed in JNeurosci do not necessarily reflect those of SfN or the JNeurosci Editorial Board. Publication of an advertisement or other product mention in JNeurosci should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in JNeurosci.