Figure 5.
Representative IHC labeling of IL-1R1 and ISSH labeling of c-fos in the PVN. A–C, No IL-1R1 labeling was found in IL-1R1 knock-out mice (A); IL-1R1 was detected in nontransgenic mice 2 h after intracerebroventricular injections of saline (B) or IL-1 (20 ng/mouse) (C). D, E, IL-1R1 was also found in eIL-1R1-water mice after intracerebroventricular injections of saline (D) and IL-1 (E). F, IL-1R1 was absent in eIL-1R1-Dox mice after intracerebroventricular injections of saline (data not shown) and IL-1. The boundaries of PVN were indicated by the dashed line. The inset in E shows a high-magnification photograph of IL-1R1 (green)- and PECAM (red)-labeled cells. a, ISSH labeling of c-fos was absent in the PVN of IL-1R1 knock-out mice after intracerebroventricular IL-1 injection. b, d, After intracerebroventricular saline injections, c-fos was absent in nontransgenic (b) and eIL-1R1-water mice (d). c, e, Two hours after intracerebroventricular IL-1 injection, c-fos expression was induced in nontransgenic (c) and eIL-1R1-water mice (e). f, In eIL-1R1-Dox mice, the induction of c-fos was absent after intracerebroventricular IL-1 injection. Scale bar, 100μm.