Article Figures & Data
Figures
- Figure 1.
D2 and D3 dopamine receptors regulate Akt phosphorylation under basal conditions. A–E, Western blots (A–D) and densitometric (E–G) analysis of phospho-Thr-308 Akt levels in extracts prepared from the striatum of different drug-naive DA receptor knock-out mice (HO) (A, D1; B, D2; C, D2L; D, D3) and WT littermates. E–G, Phospho-Thr 308 Akt (E), Phospho-Ser-9 GSK3β (F) or phospho-Ser-473 Akt (G) levels in extracts prepared from the striatum of drug-naive DA receptor knock-out mice and WT littermates. Results are presented in arbitrary units normalized to phospho-protein levels observed in WT littermates. Phospho-independent antibodies directed against respective kinases were used as loading controls. n = 5–10 mice per group; data are average ± SEM. *p ≤ 0.05, ***p ≤ 0.005.
- Figure 2.
D4 receptor blockade does not affect striatal Akt phosphorylation. Western blots (A) and densitometric analysis (B) of phospho-Akt (Thr-308) levels in striatal extracts from WT or DAT-KO mice 30 min after injection of 5 mg/kg of the D4 receptor blocker L745870. Results are presented in arbitrary units normalized to phospho-Akt levels observed in vehicle-treated mice of the same genotype. Phospho-independent antibodies directed against Akt were used as loading controls. n = 5 mice per group; data are average ± SEM.
- Figure 3.
Regulation of Akt by DA drugs in D1 and D2 receptor knock-out mice. Phospho-Thr-308 Akt levels in extracts prepared from the striatum of WT, D1, and D2 DA receptor knock-out mice injected with apomorphine (3 mg/kg) or amphetamine (3 mg/kg). Representative Western blots (A, C) show results obtained from two separate striatal extracts prepared from different mice. Analyses were conducted at 60 min after injection. Results of densitometric analysis (B, C) are presented in arbitrary units normalized to vehicle-treated mice of the same genotype. n = 5–10 mice per group; data are average ± SEM. *p ≤ 0.05, **p ≤ 0.01.
- Figure 4.
Regulation of Akt by DA drugs in D3 receptor knock-out mice. A, B, Relative phospho-Akt (Thr-308) levels in extracts prepared from the striatum of WT or D3 DA receptor knock-out mice 60 min after injection of amphetamine, 3 mg/kg (A, B) or 1 mg/kg (C, D), or of apomorphine (3 or 6 mg/kg) (E, F). Representative Western blots (A, C, E) show results obtained from two separate striatal extracts prepared from different mice. Results of densitometric analysis (B, D, F) are presented in arbitrary units normalized to vehicle-treated mice of the same genotype. n = 5–10 mice per group; data are average ± SEM. *p ≤ 0.05, **p ≤ 0.01.
