Figure 5.
ION is lost in the Ptf1a-null mice. All samples are at E18.5. A–D, Whole-mount X-gal-stained brains of indicated genotypes. Dorsal (A, B) and ventral (C, D) views. The arrow in the inset in D indicates the optic nerve and optic tract stained with X-gal. E, F, High-magnification views of C and D around the ION, respectively. An arrow in E indicates the ION. G, H, X-gal-stained sections of caudal hindbrain of indicated genotypes. I, J, High magnifications of G and H, respectively. K, L, Immunostaining with Brn3a was performed on sections of the caudal hindbrain. M, N, High-magnification views of the ventralmost regions that were stained with Brn3a. Weak false-positive signals can be observed in small blood vessels, because the antibody used was mouse monoclonal. Scale bars: A–F, 1 mm; G–N, 100 μm.
