Article Figures & Data
Figures
- Figure 1.
The postphotoreceptoral electroretinographic no-b-wave phenotype of the Gβ5−/− mice. A, Representative scotopic ERG responses of dark-adapted adult control (left) and Gβ5−/− mice (right) to a series of white flashes with indicated intensities in log cd s/m2. B, DNA construct used for the generation of TGβ5L transgenic mice. C, Western blots showing Gβ5 and RGS9-1 levels in 10 μg extracts of control, Gβ5−/−, and TGβ5L−/− mouse retinas. D, Image of a TGβ5L−/− retinal section probed with a Flag antibody showing outer segment localization of the transgenic product. OS, Outer segment; IS, inner segment. Scale bar, 10 μm. E, Representative scotopic ERG responses of dark-adapted control (left) and TGβ5L−/− mice (right), treated or untreated with APB.
- Figure 2.
Localization of Gβ5, RGS7, and RGS11 in the retina and abnormal ON-bipolar cell morphology of the Gβ5−/− mice. A–D, Retinal sections derived from control (A–C) and Gβ5−/− (D) mice probed for Gβ5 (A, D), RGS7 (B), and RGS11 (C). Sources and dilutions of antibodies are shown in Table 1. Scale bar, 10 μm. E, F, Colocalization of Goα with Gβ5 (E) and RGS11 (F) in control retinas. Scale bar, 5 μm. G, H, Retinal sections from age-matched adult control (G) and Gβ5−/− animals (H) colabeled with mGluR6 and PKCα. Scale bar, 10 μm.
- Figure 3.
Abnormal OPL ultrastructure of the Gβ5−/− mice. A, Western blot analyses of 10 μg retinal extracts of control mice at indicated postnatal ages showing relative levels of Gβ5-S and Gβ5-L in reference to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) level. B, TEM images of the OPL of the control (top) and Gβ5−/− (bottom) mice. Scale bar, 5 μm. RS, Rod spherule; CP, cone pedicle. Asterisk denotes abnormal structures found in the Gβ5−/− retina. Representative OPL synapses are shown in insets. C, D, The averaged percentage of rod spherules with normal synaptic triads (C) and ribbons (D) at indicated postnatal days. Error bars are SEM (n = 3). E, The averaged number of synaptic triads and ribbons found per cone pedicle per section in adult control and Gβ5−/− mice, n = 3. F, The averaged percentage of rod spherules with triads and ribbons in adult TGβ5L−/− mice compared with age-matched Gβ5−/− and control animals, n = 3.
Tables
Antibody Source Dilution Gβ5 (CT215) Watson et al. (1994) 1:500 RGS7 (2923AP) Chen et al. (2003) 1:100 RGS11 (αS-11) Chen et al. (2003) 1:100 RGS6 Imgenex (San Diego, CA) 1:100 Goα Millipore (Billerica, MA) 1:100 mGluR6 Neuromics (Northfield, MN) 1:100 PKCα Sigma 1:100 FLAG Sigma 1:100
