Figure 7.
Electrophysiological analysis of dlg mutants. A, B, Voltage recordings of muscle 6 in wild-type and dlg mutants, showing representative traces of mEJPs (A), and evoked responses (EJP) (B). Calibration: A, 6.5 mV, 1.7 s; B, 10 mV, 125 ms. C, D, F, G, Histograms showing the average ± SEM of mEJP amplitude (C), mEJP frequency (D), EJP amplitude (F), and quantal content (G). E, mEJP amplitude cumulative distribution. Average ± SEM of the resting membrane potential (in millivolts) and input resistance (in kiloohms · square centimeter) was 62.14 ± 0.96 and 6.35 ± 0.61 for CS, 63.38 ± 0.6 and 6.2 ± 0.53 for dlgXI-2, 63.45 ± 0.66 and 6.11 ± 0.58 for dlgA40.2, and 63.33 ± 0.41 and 6.39 ± 0.61 for dlgS975, respectively; no significant differences between genotypes were detected. Statistical analysis for the frequency, EJP amplitudes, and quantal contents was conducted using Student's t test (**p < 0.01; ***p < 0.001). Statistical analysis for the mEJP amplitude cumulative curves was conducted using a Kolmogorov–Smirnov test.