Figure 6.
CLEC-38 and RPM-1-dependent PLM axon outgrowth. Loss of clec-38 function can affect PLM axon outgrowth. A, Schematic diagram of the PLM longitudinal axon. B, C, In wild-type animals, the PLM extend to a position near the vulva. D, E, In rpm-1 mutants, the PLM axon overextends, passing the ALM cell body. F, G, In clec-38;rpm-1 mutants, the PLM axon extends beyond the AVM cell body position. The neurons were visualized with zdIs5 [mec-4::gfp] in C, E, and G. H, Loss of rpm-1 function causes overextension of PLM axon. Loss of both clec-38 and rpm-1 function cause longer extension of PLM, which crosses the AVM. This phenotype is dependent on unc-40 because the triple mutant, clec-38;rpm-1;unc-40, phenocopies the rpm-1 single mutant. Error bars indicate SE of proportions, and asterisks indicate statistically significant difference (***p < 0.0005). Anterior is to the left, and dorsal is up. Scale bars, 20 μm.