Figure 8.
Yif1B RNA interference effect is specific for the 5-HT1A receptor.
A
, No effect of Yif1B RNA interference on sst2A, P2X2, and 5-HT3A receptor distribution in the dendritic tree. Primary cultures of rat hippocampal neurons (7 DIV) were transfected with plasmids encoding the following receptors: 5-HT1A-eGFP (
a
), sst2A-eGFP (
b
), P2X2-eGFP (
c
), and YFP-5-HT3A (
d
), and either transfected alone (left), cotransfected with siRNA(Yif1B-1) (middle), or cotransfected with siRNA(Contr-1) (middle right). Immunofluorescence was performed with anti-GFP antibodies to enhance the GFP signal. Cumulated fluorescence profiles along the longest dendrite for each group (15 neurons analyzed) are shown on the right. Note the drastic reduction of fluorescence induced by siRNA(Yif1B-1) in the distal part of dendrites in 5-HT1A-eGFP-transfected neurons but not in sst2A-eGFP-, P2X2-eGFP-, or YFP-5-HT3A-transfected neurons. The higher variability in fluorescence intensities compared with Figure 7 is attributable to the lower number of neurons analyzed. Scale bar, 50 μm.
B
, Inhibition of 5-HT1A-eGFP receptor distribution in the dendritic tree by another siRNA specific to Yif1B (nucleotides 937–961). Neurons were transfected with a plasmid encoding 5-HT1A-eGFP (left), cotransfected with the siRNA(Yif1B-2) (middle), or cotransfected with the control siRNA, siRNA(Contr-2) (middle right). As above, cumulated fluorescence profiles along the longest dendrite for each group (15 neurons analyzed) are shown on the right. Scale bar, 50 μm.