Article Figures & Data
Figures
- Figure 1.
a, Photomicrograph of AAV-mediated ERα overexpression. b, Typical low levels of ERα immunoreactivity in the medial amygdala of male prairie voles. c, GFP expression. d, Graphic representation of the site of infusion (gray shaded area) (adapted from Paxinos and Franklin, 2001). Endogenous vole ERα (a) is labeled using the primary polyclonal antibody c1355 (Millipore; 1:10,000 dilution) and developed with nickel-DAB (purple staining) (Cushing et al., 2004), whereas transfected human ERα is labeled using the primary antibody RM9101-s (1:1000 dilution) and visualized using DAB (brown).
- Figure 2.
Results of treatment on the expression of spontaneous alloparental behavior. Enhancing EPα in the MeA (MeA-ERα) resulted in a significant decrease in alloparental behavior compared with control males. p < 0.001.
- Figure 3.
a, Mean time (±SE) that males spent in the cage of the familiar or novel female by treatment. b, Mean time (±SE) in side-by-side contact with the familiar and novel female by treatment. MeA-ERα males spent significantly less time in the familiar female cage and significantly more time in the novel female cage than control males (p ≤ 0.05). Within-treatment analysis revealed that MeA-ERα males displayed a preference for the novel female spending significantly more time in the novel female cage than the familiar female cage, and more time in side-by-side contact with the novel female. *p < 0.05, significant between treatment difference; line above error bars shows significant difference within treatment, p < 0.05. Error bars indicate SEM.
