Figure 1.
Axonal navigation and glial migration in the fly pupal wing.
A, B
, Schematic representation of wings before L1-glia migration (16 h APF) (
A
) and after migration completion (29 h APF) (
B
); glia in green, neurons and nerves in red. Arrow in
A
indicates direction of migration from anterior margin to the radius. Box in
B
shows the region of interest that has been analyzed by time-lapse throughout this study. L1 and L3 indicate L1 and L3 nerves respectively, GSR indicates the giant sensillum on the radius, TSM the twin sensilla on the margin, r and c correspond to radial and costal nerves, respectively.
C
shows raw data on ablated (white symbols) and not ablated (black symbols) wings, the number of samples is indicated (n). x-axis shows the different ablation conditions: 1c, 2c, 3c, 4c. y-axis indicates h APF. Each circle represents one sample; horizontal bars, MI values. Note that no MI was calculated for four-cell ablations, because migration is only accomplished in three of six wings.
D
, Summary of chain tip cell ablation data. The first column indicates the experimental conditions (control and ablated wings), the second the percentage of wings showing successful migration, the third the MI, the fourth the earliest time at which L1-glia accomplish migration, the fifth the latest time of migration accomplishment. Single asterisk, Migration is considered successful when L1-glia reach their final destination by 28 h APF. Double asterisk, MI for the pooled data (one to four cells) is underestimated, because it does not take into account the three samples of four-cell ablation in which migration is not achieved. Same for MI of four-cell ablations.